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Quick-acting hemostatic sponge and preparation method thereof

A hemostatic sponge and quick-acting technology, which is applied in the field of quick-acting hemostatic sponge and its preparation, can solve the problems of uneven polysaccharide conjugates and unsatisfactory hemostatic effect, and achieve the effects of improving hemostatic effect, accelerating wound healing, and uniform group distribution

Inactive Publication Date: 2022-05-27
SHANGHAI CHANGZHENG HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the technical problems of uneven polysaccharide conjugates added to the medical sponge and unsatisfactory hemostatic effect, the purpose of the present invention is to provide a quick-acting hemostatic sponge

Method used

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  • Quick-acting hemostatic sponge and preparation method thereof
  • Quick-acting hemostatic sponge and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0034] In this embodiment, the quick-acting hemostatic sponge comprises gelatin, a polysaccharide conjugate and a cross-linking agent, and the polysaccharide conjugate is a hydrophilic and hydrophobic co-grafted polysaccharide conjugate.

[0035] Wherein, gelatin:polysaccharide conjugate:crosslinking agent=300:100:1.

[0036] The steps of the polysaccharide conjugate in the above-mentioned quick-acting hemostatic sponge are:

[0037] 2 g of chitosan was dissolved in 100 mL of 0.2M acetic acid, and 100 mL of ethanol was added, followed by 364 uL of octanoic anhydride, and 2.38 g of EDC and 1.43 g of NHS. The reaction was overnight, and 3 mL of 5M sodium hydroxide was added to adjust the pH to 10, filtered, washed with water and ethanol for 5 times, and dried in a vacuum oven to obtain hydrophobically modified chitosan;

[0038] 2g of hydrophobically modified chitosan was dissolved in 100mL of 0.2M acetic acid, nitrogen was purged for 10 minutes, the pH was adjusted to 5.0, and...

Embodiment 2

[0045] This example is the same as Example 1 except for the co-grafted chitosan conjugate with hydrophobicity and hydrophobicity. The steps for preparing the polysaccharide conjugate are as follows:

[0046] 2 g of chitosan was dissolved in 100 mL of 0.2M acetic acid, and 100 mL of ethanol was added, heated to 45°C, followed by 0.2 g of dodecanoic anhydride, and 2.38 g of EDC and 1.43 g of NHS. The reaction was overnight, and 3 mL of 5M sodium hydroxide was added to adjust the pH to 10, filtered, washed with water and ethanol for 5 times, and dried in a vacuum oven to obtain hydrophobically modified chitosan;

[0047] 2g of hydrophobically modified chitosan was dissolved in 100mL of 0.2M acetic acid, nitrogen was purged for 10 minutes, the pH was adjusted to 5.0, and then 1.19g of 3,4-dihydroxyphenylpropionic acid, 2.38g of EDC and 1.43 g of NHS were reacted overnight, dialyzed against acidified deionized water for 3 days, and lyophilized to obtain a hydrophilic and hydrophobic ...

Embodiment 3

[0049] In this example, gelatin: polysaccharide conjugate: cross-linking agent = 500:100:1; in addition, the hydrophilic and hydrophobic co-grafted chitosan conjugate is different from Example 1, and the steps for preparing the polysaccharide conjugate are:

[0050] 2 g of chitosan was dissolved in 100 mL of 0.2M acetic acid, and 100 mL of ethanol was added, heated to 65°C, followed by 0.154 g of dodecanoic anhydride, and 2.38 g of EDC and 1.43 g of NHS. The reaction was overnight, and 3 mL of 5M sodium hydroxide was added to adjust the pH to 10, filtered, washed with water and ethanol for 5 times, and dried in a vacuum oven to obtain hydrophobically modified chitosan;

[0051] 2g of hydrophobically modified chitosan was dissolved in 100mL of 0.2M acetic acid, nitrogen was purged for 10 minutes, the pH was adjusted to 5.0, and then 1.19g of 3,4-dihydroxyphenylpropionic acid, 2.38g of EDC and 1.43 g of NHS were reacted overnight, dialyzed against acidified deionized water for 3...

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Abstract

The invention discloses a quick-acting hemostatic sponge and a preparation method thereof, the polysaccharide-based hemostatic sponge comprises gelatin, a polysaccharide conjugate and a cross-linking agent, and the polysaccharide conjugate is prepared by a hydrophilic and hydrophobic co-grafting process. Compared with a mixing scheme of a hydrophilic modified polysaccharide conjugate and a hydrophobic modified polysaccharide conjugate, the polysaccharide conjugate is more uniform in group distribution and more stable in property; the sponge can generate physical and chemical effects with all components of blood through a hydrophilic and hydrophobic synergistic effect, so that the all components of the blood are quickly gelatinized within 10-30 seconds, and the hemostatic effect of the sponge is greatly enhanced; according to the process, a polysaccharide conjugate and gelatin mixed freeze-drying mode is adopted, and the polysaccharide conjugate and the gelatin are uniformly mixed, so that the technical problem that chitosan in existing chitosan gelatin foam is easy to infiltrate so as to cause non-uniform release is solved; tannic acid is adopted as a cross-linking agent, the anti-inflammatory effect can be achieved on the premise that the biological safety of the sponge is guaranteed, the tannic acid and chitosan can achieve a synergistic effect in the hemostasis function, and the effect is better compared with that of common chitosan gelatin sponge.

Description

technical field [0001] The invention relates to a medical consumable, in particular to a quick-acting hemostatic sponge and a preparation method thereof. Background technique [0002] Uncontrollable blood loss is an important cause of death. Excessive blood loss of the injured can cause serious consequences, such as hypothermia, abnormal blood coagulation, inflammatory infection, organ failure, etc., causing permanent damage to the body or even life-threatening. Means and hemostatic materials are particularly important. [0003] In 1964, Davie and Ratnoff and others proposed the coagulation waterfall theory, which divided the coagulation process into three stages: the formation of prothrombin activator, the formation of thrombin and the formation of fibrin. Generate stimulation to activate the coagulation system. With the participation of platelets, coagulation proteins and thrombin are activated and play their roles in succession, fibrinogen is converted into fibrin, and c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L24/08A61L24/10A61L24/00
CPCA61L24/0036A61L24/104A61L24/08A61L2400/04A61L2300/21A61L2300/41A61L2300/412A61L2300/236
Inventor 职康康谭梓仪曲乐丰
Owner SHANGHAI CHANGZHENG HOSPITAL
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