Treatment method for improving regeneration efficiency of quercus robur tissue culture

A technology of tissue culture and regeneration efficiency, applied in the directions of plant regeneration, horticultural methods, botanical equipment and methods, etc., can solve the problems of slow reproduction, difficulty in introduction, and small quantity of Quercus chinensis, so as to improve the success rate of experiments and improve the experimental results. Efficiency, the effect of improving regeneration efficiency

Pending Publication Date: 2022-06-03
中国林业科学研究院经济林研究所
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Problems solved by technology

[0004] Aiming at the current technical problems of slow reproductive speed, small quantity and difficult introduction of Quercus cal

Method used

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  • Treatment method for improving regeneration efficiency of quercus robur tissue culture
  • Treatment method for improving regeneration efficiency of quercus robur tissue culture
  • Treatment method for improving regeneration efficiency of quercus robur tissue culture

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[0034] The application proposes a treatment method for improving regeneration efficiency by tissue culture of Quercus chinensis, comprising the following steps:

[0035] S1. First, pretreat the seeds of Quercus chinensis to obtain zygotic embryos with cotyledons as a culture body;

[0036] S2. After the culture body is rinsed with running water, rinsed with alcohol, rinsed with sterile water for the first time, soaked in mercuric chloride, and rinsed with sterile water for the second time, the water on the culture body is sucked dry by sterile absorbent paper, and finally the The culture body is inoculated into a sterile seedling medium for culturing and inducing sterile seedlings;

[0037] S3, the sterile seedling sprouts induced by culturing in the sterile seedling medium are divided into single sterile seedlings, and the single sterile seedlings are respectively inoculated into the proliferation medium for proliferation culture;

[0038] S4, when the sterile seedlings are ...

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Abstract

The invention provides a treatment method for improving regeneration efficiency through tissue culture of Quercus robur. The treatment method comprises the following steps: firstly, pretreating Quercus robur seeds, then inducing aseptic seedlings, then cutting the induced aseptic seedling buds into single aseptic seedlings, respectively inoculating the single aseptic seedlings into a proliferation culture medium for proliferation culture, and then carrying out rooting culture; inoculating the cotyledon embryo without the cotyledon into a callus induction culture medium for callus induction to obtain a compact callus, and performing somatic embryo induction on the compact callus. The germfree seedlings are obtained through disinfection treatment and cultivation of the quercus robur seeds, damage to tender stems of materials needed in subsequent tests due to direct disinfection of the quercus robur seedlings can be effectively avoided, and the success rate of the tests is increased; meanwhile, sterile seedlings are cultured in zygotic embryos with leaves, the sterile seedlings are cut off, induction culture is carried out, the cotyledons of the germs are cut off for continuous culture, dark culture is carried out in the whole process, and the regeneration efficiency of Quercus robur tissue culture is further improved.

Description

technical field [0001] The invention relates to the technical field of plant culture, in particular to a treatment method for improving regeneration efficiency by tissue culture of Quercus chinensis. Background technique [0002] Quercus robur L., also known as "Summer Oak" and "English Oak", is a large deciduous tree of the Fagaceae (Fagaceae) genus Quercus L., which is salt-tolerant and widely adaptable. Under the condition of thick soil layer and good water supply conditions, Quercus japonica grows vigorously and has a strong root system, and its life span can be as long as 100 years. Moreover, Quercus japonica is a precious landscaping tree species, which not only has few pests and diseases, but also can be used as medicine or feed, and has great breeding value. Due to the fast growth of the main root and the slow development of the lateral roots of the 1-3-year-old summer oak seedlings, the survival of transplanted seedlings is low, the time for slowing the seedlings i...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008Y02P60/40
Inventor 朱景乐周宗顺樊巍翟立海李慧陈梦娇任跃牛新江
Owner 中国林业科学研究院经济林研究所
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