Liquid transfer device and multi-path parallel liquid transfer device

Abstract

The embodiment of the invention discloses a liquid transfer device and a multi-path parallel liquid transfer device, the liquid transfer device comprises a liquid path valve plate, the bottom of the liquid path valve plate is provided with a valve membrane covering area, and the valve membrane covering area is provided with at least one liquid transfer channel and a plurality of valve holes; a first preset distance is formed between the valve hole and the pipetting channel; the clamping box body is fixed at the top of the liquid path valve plate, the clamping box body comprises a plurality of reagent tubes, and each reagent tube is communicated with one valve hole; the elastic valve membrane covers the valve membrane covering area; the elastic valve membrane is provided with a plurality of first preset areas, and a plurality of membrane valves are formed between the first preset areas and the liquid path valve plate; and the driving assembly is used for opening or closing the membrane valve. According to the scheme, the sample transfer process is carried out in a totally-closed state, liquid reagents can be transferred and detected in a non-negative-pressure biological experiment environment, and the risk of cross infection caused by aerosol is avoided.

Description

technical field [0001] The present disclosure relates to the technical field of biomedical instruments, in particular to a liquid transfer device and a multi-channel parallel liquid transfer device. Background technique [0002] In the production, test or detection process in the fields of biology, chemistry, food, medicine, epidemic prevention or environmental monitoring, the transfer of gas or liquid reagents is usually involved, and some liquid chemical reagents, such as ether, phenol, etc. are volatile and toxic Or, some microbial pathogens that are easy to cause infections, allergies, tumors and other diseases, such as viruses, bacteria, rickettsia, mycoplasma, chlamydia, spirochetes, fungi, actinomycetes, etc., in the transfer process, most of them use It is an open suction, transfer device, or dumping between reagent bottles. In this way, a closed operation cannot be achieved during the transfer process of the reagent, so it is easy to cause the volatile toxic reagent...

AI Technical Summary

Problems solved by technology

This method needs to be completed under negative pressure conditions, relying on the manual operation of professional testing personnel, and rigid reagent tubes such as glass are usually used in the process of sample storage, extraction or transfer, which is inconvenient for sample extraction or transfer, and the operation process is complex and automated The degree is not high, and samples are prone to cross-contamination during testing or transfer, leading to a series of problems such as false positive results; and testing personnel in an open environment are likely to increase the probability of virus infection

[0004] The second is automated detection equipment. Most of the nucleic acid automatic detection equipment currently on the market is carried out in an independent manner in the steps of nucleic acid extraction, amplification, and detection, that is, each step requires independent equipment to complete, and a nucleic acid detection The process requires multiple devices to operate

On the one hand, multiple devices take up a lot of space. On the other hand, the samples after the previous steps are completed need to be transferred to subsequent devices, which is cumbersome and time-consuming, and most of the existing nucleic acid detection devices use solenoid valves In the form of a solenoid valve to control liquid transfer, the valve core is in contact with the reagent, and the valve core needs to move, which will inevitably produce a certain gap, so the reagent may leak, and it is also vulnerable to external influence during the sample transfer process. pollution of the environment or contamination detection of the environment

[0005] In addition, the current commercialized nucleic acid detection equipment has successively appeared fully automatic nucleic acid detection equipment integrating extraction, amplification and detection, but most of them use single-channel or single-liquid transfer and detection methods, that is, detection The equipment can only extract one sample at a time for detection of a single pathogen, such as Cepheid's GeneXpert, BioMérieux's FilmArray, etc., the detection throughput and efficiency of the above products are low

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