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Citrus huanglongbing detection method based on electronic nose

A technology for citrus Huanglongbing and a detection method is applied in the field of plant disease detection, and can solve the problems of inability to achieve good detection effect, difficult application, high price and the like

Pending Publication Date: 2022-06-03
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the most reliable detection method for Huanglongbing is PCR detection, which can quantitatively detect Candida species related to citrus Huanglongbing, and is the most effective for detection of Huanglongbing. The operation is cumbersome and difficult to apply in actual production
Because citrus huanglongbing has an incubation period ranging from several months to several years, and the leaves do not have any symptoms during the incubation period, other detection methods that rely on typical symptoms of leaves, such as visual observation and detection methods based on map technology, cannot achieve relatively high good detection effect

Method used

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  • Citrus huanglongbing detection method based on electronic nose
  • Citrus huanglongbing detection method based on electronic nose
  • Citrus huanglongbing detection method based on electronic nose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The leaf samples of all examples of the present invention were obtained in November 2021 at the Citrus Research Institute of Ganzhou City, Jiangxi Province. The sample type is Gannan navel orange. The sample types include healthy samples, Huanglongbing samples, zinc-deficient samples and zinc-deficient types. Huanglongbing disease samples, the number and types of leaf samples in this example are: 40 healthy, 40 Huanglongbing, 40 zinc-deficient and 16 zinc-deficient Huanglongbing, all samples have been identified by professionals.

[0032] In this embodiment, the PEN3 electronic nose of German AIRSENSE company is used as the detection instrument. The electronic nose system 4 includes 10 metal oxide sensors, and the models and corresponding characteristics are shown in Table 1:

[0033] serial number Sensor model Sensor characteristics MOS 1 W1C Sensitive to aromatic ingredients MOS 2 W5S sensitive to nitrogen oxides MOS 3 W3C Sensi...

experiment example 1

[0053] In order to determine the best combination of feature extraction method and pattern recognition algorithm, the following experimental case 1 is implemented.

[0054] The number and types of leaf samples in this experimental case 1 are: 25 healthy and 25 Huanglongbing, all collected from the Citrus Research Institute of Ganzhou City, Jiangxi Province in November 2021.

[0055] The experimental case 1 is as follows:

[0056] Step S1: same as Step 1 of Embodiment 1;

[0057] Step S2: same as Step 2 of Embodiment 1;

[0058] Step S3: Selecting the stable value method, the extreme value method, the average value method, and the quadratic term piecewise fitting method for the response signal obtained in step S2, respectively, to perform feature extraction on the response curves of each sensor of the electronic nose;

[0059] The stable value method to extract features is to select the last second stable value of the response value of each sensor in the electronic nose as th...

experiment example 2

[0076] In order to determine the optimal gas-gathering process parameters, including gas-gathering temperature, gas-gathering time, sample volume and gas-gathering space, the following experimental case 2 was implemented.

[0077] The number and types of leaf samples in Case 2 of this experiment are: 72 healthy and 72 Huanglongbing, all collected from the Citrus Research Institute of Ganzhou City, Jiangxi Province in November 2021;

[0078] The experimental case 2 is as follows:

[0079] Step A1: Collect the headspace volatiles of citrus leaves with different disease species through the gas collecting device, specifically:

[0080] (1) Optimization of gas gathering temperature parameters

[0081] Place the 200ml beaker 1 in an incubator, adjust the temperature of the incubator, and divide it into 3 groups according to the gas gathering temperature: 20°C, 40°C, and 60°C, each group experiment with 6 samples, take healthy and Huanglongbing leaf samples, each beaker Put 0.2g of...

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Abstract

The invention provides a Citrus Huanglongbing detection method based on an electronic nose. The method comprises the following steps: collecting headspace volatile matters of different disease types of citrus leaves through a gas collection device; cleaning a sealed cavity of the electronic nose; collecting headspace volatile matters in the gas collection device, and injecting the headspace volatile matters into the electronic nose; performing feature extraction on the response curve of each sensor in the electronic nose by using an extreme value method; taking the extracted features as original data, and selecting a linear discriminant analysis algorithm to establish a mode recognition model; and 6, repeating the previous four steps to extract the feature data of the to-be-detected sample, inputting the features of the to-be-detected sample into the mode recognition model established in the step 5, and predicting whether the citrus leaves are infected with the Huanglongbing. According to the method, the problem that zinc deficiency and zinc deficiency type huanglongbing cannot be accurately detected in the current huanglongbing detection field is solved, different stress (huanglongbing, zinc deficiency and zinc deficiency type huanglongbing) of citrus can be accurately distinguished by using the electronic nose, and the method has practical application value.

Description

technical field [0001] The invention relates to the field of detection of plant diseases, in particular to an electronic nose-based detection method for citrus Huanglongbing. Background technique [0002] Citrus huanglong is highly contagious and destructive, which has a devastating impact on citrus production. After citrus plants are infected with Huanglongbing, they will produce specific volatile organic compounds, which attract psyllids to suck, and then spread to the whole orchard through psyllids, which is extremely contagious. The disease causes citrus plants to show mottled leaves, weak tree vigor, red nose fruit or green fruit that does not change color, etc. Once the plant is infected with Huanglongbing, the quality of the fruit will be seriously affected and the edible value will be lost, and the citrus plant will die in severe cases. In my country, Huanglongbing disease spreads almost all over the citrus producing areas, which seriously restricts the development ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/00G06K9/62
CPCG01N27/00G06F18/285Y02A90/10
Inventor 蔡健荣许骞孙力白竣文李子其谭彬
Owner JIANGSU UNIV
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