Citrus huanglongbing detection method based on electronic nose
A technology for citrus Huanglongbing and a detection method is applied in the field of plant disease detection, and can solve the problems of inability to achieve good detection effect, difficult application, high price and the like
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Embodiment 1
[0031] The leaf samples of all examples of the present invention were obtained in November 2021 at the Citrus Research Institute of Ganzhou City, Jiangxi Province. The sample type is Gannan navel orange. The sample types include healthy samples, Huanglongbing samples, zinc-deficient samples and zinc-deficient types. Huanglongbing disease samples, the number and types of leaf samples in this example are: 40 healthy, 40 Huanglongbing, 40 zinc-deficient and 16 zinc-deficient Huanglongbing, all samples have been identified by professionals.
[0032] In this embodiment, the PEN3 electronic nose of German AIRSENSE company is used as the detection instrument. The electronic nose system 4 includes 10 metal oxide sensors, and the models and corresponding characteristics are shown in Table 1:
[0033] serial number Sensor model Sensor characteristics MOS 1 W1C Sensitive to aromatic ingredients MOS 2 W5S sensitive to nitrogen oxides MOS 3 W3C Sensi...
experiment example 1
[0053] In order to determine the best combination of feature extraction method and pattern recognition algorithm, the following experimental case 1 is implemented.
[0054] The number and types of leaf samples in this experimental case 1 are: 25 healthy and 25 Huanglongbing, all collected from the Citrus Research Institute of Ganzhou City, Jiangxi Province in November 2021.
[0055] The experimental case 1 is as follows:
[0056] Step S1: same as Step 1 of Embodiment 1;
[0057] Step S2: same as Step 2 of Embodiment 1;
[0058] Step S3: Selecting the stable value method, the extreme value method, the average value method, and the quadratic term piecewise fitting method for the response signal obtained in step S2, respectively, to perform feature extraction on the response curves of each sensor of the electronic nose;
[0059] The stable value method to extract features is to select the last second stable value of the response value of each sensor in the electronic nose as th...
experiment example 2
[0076] In order to determine the optimal gas-gathering process parameters, including gas-gathering temperature, gas-gathering time, sample volume and gas-gathering space, the following experimental case 2 was implemented.
[0077] The number and types of leaf samples in Case 2 of this experiment are: 72 healthy and 72 Huanglongbing, all collected from the Citrus Research Institute of Ganzhou City, Jiangxi Province in November 2021;
[0078] The experimental case 2 is as follows:
[0079] Step A1: Collect the headspace volatiles of citrus leaves with different disease species through the gas collecting device, specifically:
[0080] (1) Optimization of gas gathering temperature parameters
[0081] Place the 200ml beaker 1 in an incubator, adjust the temperature of the incubator, and divide it into 3 groups according to the gas gathering temperature: 20°C, 40°C, and 60°C, each group experiment with 6 samples, take healthy and Huanglongbing leaf samples, each beaker Put 0.2g of...
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