Nucleic acid pre-denaturation system and method for performing pre-denaturation treatment on nucleic acid

Abstract

The invention discloses a nucleic acid pre-denaturation system and a method for performing pre-denaturation treatment on nucleic acid. The nucleic acid pre-denaturation system comprises: a feeder for providing a liquid raw material with nucleic acid; the first heat exchanger is used for heating the liquid from the feeder; the incubator is separated from the first heat exchanger and is used for incubating the liquid from the first heat exchanger; and a second heat exchanger separate from the incubator for cooling the liquid from the incubator. According to the nucleic acid pre-denaturation system provided by the invention, the efficiency of nucleic acid pre-denaturation can be improved.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a nucleic acid pre-denaturation system and a method for pre-denaturing nucleic acid. Background technique [0002] In small-scale research in the laboratory, when performing PCR or reverse transcription reactions on nucleic acids, such as DNA or RNA, for example, use a 1.5ml centrifuge tube, use a metal bath or water bath to heat up quickly (for example, within 5 minutes), and cool down by Place the centrifuge tube in ice or a low temperature environment to achieve rapid cooling (eg, within 5 minutes). However, as the volume of the pre-denaturation reaction increases, it is a huge challenge to process it in a 1.5ml centrifuge tube. The temperature-changing reactors currently on the market usually use the TCU-jacket / heating blanket to control the temperature. It usually takes about 30 minutes to heat up and down, and the volume is usually fixed, such as Sartorius’ glass reactor Univessel®...

AI Technical Summary

Problems solved by technology

However, as the volume of the pre-denaturation reaction increases, it is a huge challenge to process it in a 1.5ml centrifuge tube

The temperature-changing reactors currently on the market usually use the TCU-jacket / heating blanket to control the temperature. It usually takes about 30 minutes to heat up and down, and the volume is usually fixed, such as Sartorius’ glass reactor Univessel® Glass , a glass jacket is added outside the reactor, and water and ethylene glycol are added to the space between the jacket and the reactor. The temperature of water and ethylene glycol is raised or lowered by other temperature control units, and the temperature of the solution in the reactor is therefore It is controlled, but usually the speed of heating and cooling gradually decreases with the increase in volume. If the cooling time is too long, DNA or RNA may re-form secondary structures, affecting the reaction efficiency, and the stability of DNA or RNA may also be reduced. If the volume is too small, it cannot meet the production needs of a single batch

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