Analysis method for simultaneously quantifying 15 ginsenoside components in Danqi Tongmai tablet in rat plasma
A technology of ginsenosides and analysis method is applied in the field of simultaneous quantitative analysis of 15 kinds of ginsenosides in Danqitongmai tablets in rat plasma, which can solve the problems of low response, unknown specific concentration, low specificity and sensitivity, etc. Achieve good reproducibility, improve signal-to-noise ratio and detection sensitivity, and reduce the number of transitions
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Embodiment 1
[0048] Simultaneously quantify 15 ginsenosides in Danqitongmai Tablets in rat plasma by the following method, and the method includes the following steps:
[0049] (1) Preparation of single reference stock solution: Precisely weigh ginsenosides Rk3, 20(S)-Rh1, 20(R)-Rh1, NR2, Rg2, Rg3, Rg1, NR1, Rd, NK, Re, 20 -O-Glc-Rf, Rb3, Rb1, NR4 each 5mg, placed in a 5mL volumetric flask, add methanol to the mark, and prepare a single reference stock solution with a concentration of 1mg / mL;
[0050] (2) Preparation of standard curve working solution: respectively draw an appropriate volume of the above single reference substance stock solution into a 5mL volumetric flask, add methanol to volume to the mark, and prepare a mixed reference substance stock solution with each reference substance concentration of 40 μg / mL ; Draw an appropriate volume of the above mixed standard stock solution and add methanol to dilute step by step to obtain a standard curve working solution with concentration...
Embodiment 2
[0057] Example 2: Methodological investigation
[0058] (1) Standard curve and linear range: According to the establishment method of the standard curve, take the concentration of each analyte reference substance in plasma as the abscissa, and the ratio of the peak area of the analyte to the peak area of the internal standard as the ordinate, using 1 / x 2 The weighted least squares method was used for linear regression, and the standard curve equation and linear range of each analyte were shown in Table 2.
[0059] Table 2 Standard curves and linear ranges of 15 ginsenosides
[0060]
[0061] (2) Accuracy and precision: LQC, MQC and HQC were used to evaluate the intra-day and intra-day accuracy and precision. The relative standard deviation of the accuracy and precision was less than 15%, indicating that the established method was accurate and reliable. The intra-day and inter-day precision and accuracy are shown in Tables 3 and 4, respectively.
[0062] Table 3 Intr...
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