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Construction method of mouse talaromyces marneffei latent reactivation model

A Marneffei and construction method technology, applied in the field of bioengineering, can solve problems such as inappropriate methods for establishing latent infection models of pathogens, and no experimental mouse models for reactivating infection have been retrieved, so as to broaden research ideas and speed up experimental research. The effect of short progress and modeling cycle

Pending Publication Date: 2022-06-24
GUANGXI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, there is no relevant report on how to construct an experimental mouse model of TM latent reactivation infection
It is worth noting that the pathogenesis of viruses, bacteria and fungi are different, and TM, as a systemic invasive fungus, is different from the pathogenesis of viruses and bacteria, so it is not suitable for the establishment of the above-mentioned pathogen latent infection model

Method used

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  • Construction method of mouse talaromyces marneffei latent reactivation model
  • Construction method of mouse talaromyces marneffei latent reactivation model
  • Construction method of mouse talaromyces marneffei latent reactivation model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] This example mainly introduces a method for constructing a latent reactivation model of T. marneffei in mice.

[0048] Please refer to figure 1 shown.

[0049] S1. Construction of TM-infected mice:

[0050] ① Animal feeding: After obtaining the approval of animal ethics, the mice are kept in the animal biosafety laboratory. The humidity of the animal room is constant and the temperature is 18-25 °C. During the feeding process, artificial day and night are adopted to feed the mice and sterilize them. Distilled water.

[0051] ②TM infected mice: The purified standard strain of TM 18224 was obtained from the American Type Culture Collection (ATCC). Take out the TM standard strain bacterial suspension refrigerated at 4 °C, and the concentration of the bacterial suspension can be determined by direct counting with a hemocytometer or turbidimetric method, and the concentration is 10 8 CFU / mL, mouse TM infection was achieved by tail vein injection of 0.2 mL of the suspensi...

Embodiment 2

[0063] On the basis of Embodiment 1, this embodiment further introduces the construction method of the model.

[0064] Please refer to figure 2 shown.

[0065] First, Balb / c mice were selected, and the mice were 6-8 week old female mice. Then, on day 0, the mice were acclimated to the environment, and ear tag forceps were used to make holes and mark them for subsequent experiments.

[0066] On the first day, the standard strain of TM was used to administer the mice, and the physiological indexes of the mice were regularly monitored, and the physiological indexes included: the weight of the mice, the amount of bacteria in the organs, the coefficient of the organs and the morphological identification of the TM. When the indicators indicated that the mice had been infected with TM, it indicated that the TM-infected mice were successfully constructed, and then the TM-infected mice were continuously raised.

[0067] The continuous feeding time is 7n days, and n is a natural num...

Embodiment 3

[0078] This example introduces the process of constructing TM-infected mice in step S1.

[0079] Please refer to image 3 , Figure 4 shown.

[0080] Step S1, construct TM-infected mice.

[0081] After obtaining the approval of animal ethics, the mice were raised in the animal biosafety laboratory. The humidity of the animal room was constant and the temperature was 18-25 °C. During the raising process, artificial day and night were adopted, and the mice were fed with feed and sterilized distilled water. The mice used in this experiment were Balb / c mice, and female mice were 6-8 weeks old.

[0082] On day 0, mice were acclimated and punched and marked using ear tag forceps. Then, from day 0, mice were weighed and data recorded daily for subsequent experiments.

[0083] On the first day, take out the bacterial suspension of the TM standard strain refrigerated at 4 °C, and the concentration of the bacterial suspension can be determined by direct counting with a hemocytomete...

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Abstract

The invention relates to a construction method of a mouse talaromyces marneffei latent reactivation model, which comprises the following steps: constructing a TM infected mouse, performing immunosuppression intervention on the TM infected mouse, and finally detecting the TM latent reactivation state of the mouse to realize the construction of the model. The talaromyces marneffei latent reactivation model of the mouse is successfully established for the first time, and the TM latent reactivation model is established in the mouse body, so that the dynamic process of fungi in the human body is simulated, and a model basis is provided for researching the latent infection state of TM in the body. And the TM latent activation model is used as a tool model for researching the TM, so that the molecular mechanism of TM latent infection can be further clarified conveniently, a sufficient basis and an early-stage basis are provided for analyzing the pathogenesis of the TM, a tool basis is also provided for deeply excavating the TM latent infection mechanism, and the research and development of related drugs in the field are promoted.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a method for constructing a latent reactivation model of T. marneffei in mice. Background technique [0002] Talaromyces marneffei (TM) is a species of Talaromyces and the only known pathogenic bacteria in the genus Talaromyces so far. Talaromycosis is caused by TM. A systemic invasive fungal disease caused by infection. TM infection is a regional high-incidence opportunistic infection, mainly prevalent in Southeast Asia and southern China. Cellular immunodeficiency is a high risk factor for TM infection. In HIV-infected population, coinfection with TM will accelerate the deprivation of life and health in AIDS patients. According to reports, 56.6% of the reported cases of TM disease in my country occurred in Guangxi, and the death risk of HIV / TM infection in Guangxi ranked first among all opportunistic infections (AHR=4.51). In recent years, the number of non-HIV-infect...

Claims

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Application Information

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IPC IPC(8): A01K67/02A61K49/00
CPCA01K67/02A61K49/0008Y02A50/30
Inventor 叶力王刚袁宗祥蒋俊俊韦吴迪宁传艺周波张洪何锦豪陈丽香刘宇轩
Owner GUANGXI MEDICAL UNIVERSITY
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