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Breeding method for enhancing disease resistance of tomatoes

A technology for disease resistance and tomato, which is applied in the field of breeding to enhance tomato disease resistance, and can solve the problem that disease-resistant tomatoes take a long time

Pending Publication Date: 2022-06-28
ANHUI JINPEIYIN TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the change of global climate and the growth of population, higher requirements have been put forward for the yield, disease resistance and stress resistance of tomato. However, the cultivation of disease-resistant tomato takes a long time, so it is urgent to cultivate Tomato method for faster determination of disease resistance

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A breeding method for enhancing disease resistance of tomato, comprising the following steps:

[0023] Step 1. First, thoroughly inspect and clean up the miscellaneous plants of the parent Honda. If the plant type, leaf type, leaf color, flower and fruit are different, all should be pulled out to ensure the purity of the seeds;

[0024] Step 2, after the impurity removal in step 1 is completed, inoculate the original bacteria, and inoculate 20 strains of each type of bacteria respectively, and keep 20 uninoculated strains as a control group;

[0025] Step 3: After inoculating the original bacteria in step 2, carry out the PCR method for viral nucleic acid detection every 3 days, and obtain the seeds of the preselected disease-resistant and non-disease-resistant tomato strains and the disease-resistant strains as the female parent and the non-disease-resistant strain as the male parent. The first-generation seeds of this hybrid, the second-generation seeds formed by self...

Embodiment 2

[0032] A breeding method for enhancing disease resistance of tomato, comprising the following steps:

[0033] Step 1. First, thoroughly inspect and clean up the miscellaneous plants of the parent Honda. If the plant type, leaf type, leaf color, flower and fruit are different, all should be pulled out to ensure the purity of the seeds;

[0034] Step 2, after the impurity removal in step 1 is completed, inoculate the original bacteria, and inoculate 30 strains of each type of bacteria respectively, and retain 30 uninoculated strains as a control group;

[0035] Step 3, after inoculating the original bacteria in step 2, carry out the PCR method for viral nucleic acid detection every 6 days, and obtain the seeds of the preselected disease-resistant and non-disease-resistant tomato strains and the disease-resistant strain as the female parent and the non-disease-resistant strain as the parent. The first-generation seeds of this hybrid, the second-generation seeds formed by self-cro...

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PUM

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Abstract

The invention discloses a breeding method for enhancing disease resistance of tomatoes, and relates to the technical field of tomato breeding, the breeding method comprises the following steps: obtaining seeds of a preselected disease-resistant and non-disease-resistant tomato strain and first-generation seeds hybridized by taking a disease-resistant strain as a female parent and a non-disease-resistant strain as a male parent through inoculation of virus protobacteria, and carrying out selfing to form second-generation seeds; the three-leaf seedlings cultivated by two parents and second-generation seeds are used for establishing a gene pool, so that the tomato disease-resistant breeding time can be greatly shortened, and whether disease-resistant genes exist or not can be determined within several months.

Description

technical field [0001] The invention relates to the technical field of tomato breeding, in particular to a breeding method for enhancing the disease resistance of tomato. Background technique [0002] China is a big country in vegetable production and consumption, of which tomatoes account for about 7% of vegetable production. The total tomato production in 2020 is 6.515x1010kg. Tomatoes have high nutritional value, such as rich in vitamins, carotene and lycopene, and these substances have anti-cancer and anti-aging effects. With the change of global climate and the growth of population, higher requirements are put forward for tomato yield, disease resistance, stress resistance and other traits, but the cultivation of disease-resistant tomato currently takes a long time, so there is an urgent need to cultivate plants that can A quicker way to determine if a tomato is disease resistant. SUMMARY OF THE INVENTION [0003] The purpose of the present invention is to solve the...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04C12Q1/18C12Q1/6858C12Q1/6895
CPCA01H1/02A01H1/04A01H1/126C12Q1/18C12Q1/6858C12Q1/6895C12Q2600/156C12Q2600/13C12Q2521/301C12Q2531/113
Inventor 范思静王亚男樊友鑫
Owner ANHUI JINPEIYIN TECH