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Cx43 expression related biomolecule, sepsis treatment target and prognostic marker

A biomolecular and sepsis technology, applied in the field of molecular biology, can solve the problem of unclear pathogenesis of sepsis

Pending Publication Date: 2022-06-28
SHENZHEN PEOPLES HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The embodiment of the present invention provides a biomolecule related to the expression of Cx43, a sepsis treatment target and a prognostic marker, aiming to solve the technical problem in the prior art that the pathogenesis of sepsis is not yet clear

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  • Cx43 expression related biomolecule, sepsis treatment target and prognostic marker
  • Cx43 expression related biomolecule, sepsis treatment target and prognostic marker
  • Cx43 expression related biomolecule, sepsis treatment target and prognostic marker

Examples

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Embodiment 1

[0057] Figure 1a It is a schematic diagram of the detection result of the fluorescence quantitative PCR provided in the embodiment of the present invention. Figure 1b Schematic diagram of exosome identification results. Figure 1c Schematic diagram of the verification experimental results of the dual-luciferase reporter gene.

[0058] In this example, an appropriate number of healthy people (17 people) and sepsis patients (51 people) were divided into the control group and the experimental group, respectively. Then the exosomes in the peripheral blood of the experimental group and the control group were extracted. Finally, the quantitative detection of miR-1-3p expression in the experimental group and the control group was carried out by using the method of real-time quantitative PCR. like Figure 1a As shown, compared with the healthy people in the control group, the expression of miR-1-3p in the peripheral blood of the experimental group (ie, sepsis patients) was signifi...

Embodiment 2

[0063] To further verify the association between Cx43 and miR-1-3p and sepsis, the embodiment of the present invention also provides a validation experiment based on a sepsis mouse model. in, Figure 2a are the fluorescence quantitative PCR results of miR-1-3p provided in the embodiment of the present invention, Figure 2b are the fluorescence quantitative PCR results of Cx43 provided in the embodiment of the present invention, Figure 2c is the fluorescence quantitative PCR result of TGFβR provided in the embodiment of the present invention, Figure 2d is a schematic diagram of the concentration of TGFβ provided in the embodiment of the present invention, Figure 2e is a schematic diagram of the results of the WB detection provided by the embodiment of the present invention, Figure 2f It is a schematic diagram of the results of HE staining and immunohistochemistry of the sepsis mouse model provided in the embodiment of the present invention.

[0064] In the present examp...

Embodiment 3

[0073] To further verify the relationship between Cx43 and miR-1-3p and sepsis, the embodiment of the present invention also provides a validation experiment of a sepsis model constructed based on HCT116 cells. in, Figure 3a The fluorescence quantitative PCR results of HCT116 cells provided in the embodiment of the present invention, Figure 3b and 3c A schematic diagram of the WB detection results of HCT116 cells provided in the embodiment of the present invention, Figure 3d and 3e The schematic diagram of the ELISA detection result of TGFβ expression in the cell culture supernatant provided by the embodiment of the present invention, Figure 3f The schematic diagram of the test results of CCK-8 detection of cell proliferation and cell migration method (transwell) detection of cell invasion ability provided in the embodiment of the present invention.

[0074] In this example, a sepsis cell model was simulated by adding LPS (Lipopolysaccharide, lipopolysaccharide) to HCT...

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Abstract

The embodiment of the invention provides a biomolecule related to Cx43 expression, a sepsis treatment target and a sepsis prognosis marker. Wherein the biomolecule comprises a sequence as shown in SEQ ID No. 1; or at least one basic group in the sequence as shown in SEQ ID No.1 is varied, and the sequence as shown in SEQ ID No.1 has a variation sequence acting on the same target molecule; 1, or an RNA fragment containing a sequence as shown in SEQ ID No. 1 or a variant sequence of the sequence as shown in SEQ ID No. 1. The biomolecule is related to expression of Cx43, participates in a molecular regulation mechanism of sepsis, and can be used as a sepsis treatment target or a prognostic marker of sepsis.

Description

【Technical field】 [0001] The invention relates to the technical field of molecular biology, in particular to a biomolecule related to Cx43 expression, a sepsis treatment target and a prognosis marker. 【Background technique】 [0002] Sepsis is a systemic inflammatory response syndrome (SIRS) caused by infection. It may be caused by infection in any part, and clinically it is common in pneumonia, peritonitis, cholangitis, urinary tract infection, cellulitis, meningitis, abscess, etc. [0003] Sepsis is a dangerous disease with a high mortality rate. Some surveys have shown that the fatality rate of sepsis has surpassed that of myocardial infarction, becoming the main cause of death in non-cardiac patients in intensive care units. [0004] In recent years, despite great progress in anti-infective therapy and organ function support technology, the fatality rate of sepsis is still as high as 30% to 70%. The high cost of sepsis treatment and the large consumption of medical res...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12Q1/6883A61K45/00A61P31/00
CPCC12N15/113C12Q1/6883A61K45/00A61P31/00C12N2310/141C12Q2600/118C12Q2600/158C12Q2600/178
Inventor 陈怀生王薇李富荣刘雪燕刘婓渊洪澄英
Owner SHENZHEN PEOPLES HOSPITAL