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MiRNA marker for detecting and evaluating high-intensity endurance training effect or combination thereof and application thereof

A technology of endurance training and markers, which is applied in the direction of biochemical equipment and methods, microbiological measurement/testing, etc., can solve the problems of inaccurate response regulation mechanism, etc., and achieve high sensitivity and good specificity

Pending Publication Date: 2022-06-28
NANJING YOUZHIYUAN PHARMACEUTICAL TECHNOLOGY CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional indicators reflect the physiological response of the body after endurance exercise at the physiological level, and cannot accurately reflect what kind of regulatory mechanism occurs in the body, thus bringing about a series of biological adaptations.

Method used

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  • MiRNA marker for detecting and evaluating high-intensity endurance training effect or combination thereof and application thereof
  • MiRNA marker for detecting and evaluating high-intensity endurance training effect or combination thereof and application thereof
  • MiRNA marker for detecting and evaluating high-intensity endurance training effect or combination thereof and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: Reflecting the state of the body by detecting the changes of high-intensity endurance exercise load and heart rate variability index.

[0036](1) The research subjects were 18 adult men with regular training background. All subjects had no neuromuscular, metabolic, hormonal and cardiovascular-related diseases. There was no medication record during the experiment. One week before the experiment, they stopped training for one week, and had a unified work and rest diet. , two days before the start of the experiment, the maximal oxygen uptake of the body was measured by the incremental load program (Bruce program), and the heart rate and heart rate variability indicators in the resting state (10min lying down) were measured (the subject information is shown in Table 1). The acute high-intensity endurance training adopts the 8km full-scale running program. The subjects conduct the experiment after 4 pm. During the exercise, the heart rate, load, and heart rate vari...

Embodiment 2

[0046] Example 2 The content of blood lactate and CK, CK-MB, cTnI and Mb in serum was detected to determine the metabolic characteristics of high-intensity endurance exercise and whether it caused muscle damage and whether its adaptation was a physiological adaptation induced by exercise.

[0047] After collecting the serum of the subjects, the contents of the above-mentioned several markers were determined in time on the same day ( figure 1 ). The increase of blood lactate immediately after exercise indicates that there is a small part of anaerobic glycolysis for energy supply during 8km high-intensity endurance exercise (A); among the results of the four injury markers, Mb, CK-MB and cTnI were significantly increased during exercise. Although it has increased at different time points, its expression level is still within the normal physiological range (ADE), indicating that after 8 km of high-intensity endurance exercise, the adaptation is mainly induced by physiological cha...

Embodiment 3

[0048] Example 3 Detection of time-series changes of four muscle-specific miRNAs in plasma.

[0049] After collecting the subject's plasma, reverse transcription was performed using a miRNA-specific reverse primer to obtain a cDNA containing a common stem-loop structure but belonging to a specific miRNA (product of ABI Company). The cDNA reverse reaction system of miRNA is: 2 μl RNA, 2 μl 5×AMV buffer, 1 μl 10 mmol various dNTP mixtures (Takara Company), 0.5 μl AMV (Takara Company) and 1 μl miRNA mature reverse transcription primer (provided by American ABI Company, dedicated For miRNA reverse transcription), make up the volume to 10 μl with 3.5 μl DEPC water. Afterwards, Real-time PCR reaction was performed, and the results showed that the expressions of miR-1, miR-133a, miR-133b and miR-206 in plasma before and after exercise (Pre), immediately after exercise (0h) and 24h after exercise quantity. Immediately after acute high-intensity endurance exercise, the levels of miR-...

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Abstract

The invention discloses a miRNA marker for detecting and evaluating a high-intensity endurance training effect or a combination thereof and an application of the miRNA marker. The high-intensity endurance exercise marker provided by the invention contains four types of muscle-specific micro ribonucleic acids which stably exist in plasma and can be detected. The single or combined method provided by the invention can be used for assisting in precise monitoring of human body endurance exercise load and monitoring of a recovery period, has the advantages of high sensitivity, low detection cost, convenience in material taking, easiness in sample storage and the like compared with a traditional muscle injury marker, and can be widely applied to endurance exercise monitoring, overtraining prevention, high accuracy and high reliability. The defects that traditional indexes are insufficient in specificity and accuracy are overcome, and a reliable means is provided for achieving individualized and optimal training.

Description

technical field [0001] The invention belongs to the field of biotechnology, and designs a miRNA marker or a combination thereof for detecting and evaluating the effect of high-intensity endurance training and its application. Background technique [0002] Endurance exercise promotes health and improves endurance exercise ability. Endurance exercise has a significant effect in the prevention and treatment of chronic diseases such as diabetes and cardiovascular disease. Endurance exercise induces a series of physiological adaptations, which play an important role in promoting cardiac function and the body's aerobic capacity [1] . Endurance exercise produces a series of stimuli to the body, causing a series of biological events. In order to achieve the purpose of optimizing training, some non-invasive monitoring methods are used to monitor continuous training load changes, timely reflect the physiological changes of the body, improve exercise ability and avoid overtraining [...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888
CPCC12Q1/6888C12Q2600/178C12Q2600/158
Inventor 殷鑫徐盛嘉徐琳郑益丽
Owner NANJING YOUZHIYUAN PHARMACEUTICAL TECHNOLOGY CO LTD
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