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PCR nucleic acid detector

A detector and nucleic acid technology, applied in the field of PCR instruments, can solve the problems of complex structure and low heating and cooling rate, and achieve the effects of high heating and cooling rate, easy installation, and improved detection efficiency.

Pending Publication Date: 2022-07-01
上海驷格生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In view of the above-mentioned shortcomings of the prior art, the purpose of the present invention is to provide a PCR nucleic acid detector, which is used to solve the problems of low heating and cooling rate and complex structure in the process of heating and exciting the sample in the prior art

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Embodiment Construction

[0054] The embodiments of the present invention are described below by specific embodiments, and those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification.

[0055] see Figure 1 to Figure 7 . It should be noted that the structures, proportions, sizes, etc. shown in the accompanying drawings in this specification are only used to cooperate with the contents disclosed in the specification for the understanding and reading of those who are familiar with the technology, and are not intended to limit the implementation of the present invention. Restricted conditions, it does not have technical substantive significance, any structural modification, proportional relationship change or size adjustment, without affecting the effect that the present invention can produce and the purpose that can be achieved, should still fall within the present invention. The scope of the disclosed technical c...

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Abstract

The invention provides a PCR (Polymerase Chain Reaction) nucleic acid detector, which comprises a shell, a heating and cooling module arranged in the shell, an imaging detection assembly and a TEC (Thermoelectric Cooler) heating chip in the heating and cooling module form an objective table, and the objective table is used for placing a PCR chip to be detected; the imaging detection assembly is arranged above the objective table, the imaging detection assembly comprises a yellow light assembly, a camera shooting assembly and a blue light assembly, the yellow light assembly and the blue light assembly are arranged on the two sides of the camera shooting assembly, and the camera shooting focus of the camera shooting assembly is located on the objective table; and emergent light spots of the yellow light assembly and the blue light assembly are positioned on the objective table. The device is compact in structure and high in detection efficiency.

Description

technical field [0001] The invention relates to a PCR instrument, in particular to a PCR nucleic acid detector. Background technique [0002] Polymerase Chain Reaction (PCR for short) is a molecular biology technique used to amplify specific DNA fragments. It can be regarded as a special DNA replication in vitro. The biggest feature of PCR is that it can Trace amounts of DNA increased substantially. [0003] Real-time fluorescence quantitative PCR technology is a method of adding fluorescent groups to the PCR reaction system, using the accumulation of fluorescent signals to monitor the entire PCR process in real time, and finally quantitatively analyzing the unknown template through the standard curve. Real-time fluorescence quantitative PCR technology effectively solves the limitation of traditional quantification that can only be detected at the end point, and realizes that the intensity of the fluorescent signal is detected once in each cycle and recorded in the software...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12M1/38C12M1/34
CPCB01L3/5027B01L7/52B01L2300/1805B01L2300/0654
Inventor 庞琪彭铁刚
Owner 上海驷格生物科技有限公司
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