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Paeonia lactiflora and liquorice decoction fingerprint spectrum, construction method thereof and detection method of peony and liquorice decoction product

A peony gancao decoction and fingerprinting technology, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of complex components of traditional Chinese medicine, not meeting the requirements of quality control of traditional Chinese medicine, and the effective part is not a single component, etc., to achieve good peak shape , high precision, no interference effect of peak shape

Pending Publication Date: 2022-07-01
TONG REN TANG TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The composition of traditional Chinese medicine is complex, and the effective part is often not a single component. Using a single component as a quality control indicator has become less and less suitable for the quality control requirements of traditional Chinese medicine.

Method used

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  • Paeonia lactiflora and liquorice decoction fingerprint spectrum, construction method thereof and detection method of peony and liquorice decoction product
  • Paeonia lactiflora and liquorice decoction fingerprint spectrum, construction method thereof and detection method of peony and liquorice decoction product
  • Paeonia lactiflora and liquorice decoction fingerprint spectrum, construction method thereof and detection method of peony and liquorice decoction product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0115] The API composition of Shaoyao Gancao Decoction Granules is: 12 g of white peony root and 12 g of licorice;

[0116] The preparation method is as follows: respectively taking selected weights of white peony root and licorice root, adding 600 ml of water, decocting for 75 minutes, filtering, concentrating the filtrate under reduced pressure to a relative density of about 1.3 at 60° C., drying, adding conventional auxiliary materials, and following conventional methods. process to make granules.

Embodiment 2

[0118] The present embodiment provides a method for constructing the fingerprint of Shaoyao Gancao Decoction medicinal preparation, comprising the following steps:

[0119] Take 0.1g of the drug preparation to be tested, accurately weigh it, put it in a 50mL conical flask with stopper, add 50ml of methanol precisely, weigh it, ultrasonically treat it for 30 minutes, let it cool, weigh it again, and use methanol to make up for the lost weight. , shake well, filter, and take the filtrate to get the test solution;

[0120] Take liquiritin and add 70% ethanol aqueous solution to make a solution containing 30 μg of liquiritin per 1 mL, to obtain the reference solution;

[0121] Using octadecylsilane-bonded silica gel as filler, acetonitrile as mobile phase A, and 0.1% phosphoric acid solution as mobile phase B, the gradient elution was carried out according to the following procedure: 0-1min, A:B ratio of 9%:91 %; 1-28min, A:B is 9%:91%→52%:48%; 28-33min, A:B is 52%:48%; flow rate...

Embodiment 3

[0124] The present embodiment provides a method for constructing the fingerprint of Shaoyao Gancao Decoction medicinal preparation, comprising the following steps:

[0125] Take 0.2 g of the drug preparation to be tested, accurately weigh it, put it in a 50 mL conical flask with a stopper, accurately add 50 mL of 70% methanol aqueous solution, weigh it, ultrasonically treat it for 40 minutes, let it cool, weigh it again, and make up for it with methanol. The lost weight is shaken, filtered, and the filtrate is taken to obtain the test solution;

[0126] Take an appropriate amount of liquiritin, add 70% ethanol aqueous solution to make a solution containing 40 μg of liquiritin per 1 mL, that is, the reference solution;

[0127] Using octadecylsilane-bonded silica gel as filler, acetonitrile as mobile phase A, and 0.1% phosphoric acid solution as mobile phase B, gradient elution was carried out according to the following procedure: 0-1min, A:B is 10%:90 %; 1-28min, A:B is 10%:9...

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Abstract

The invention belongs to the field of medicine analysis, and particularly provides a paeonia lactiflora and liquorice decoction fingerprint spectrum and a construction method thereof and a quality detection method of paeonia lactiflora and liquorice decoction. In the construction method of the paeonia lactiflora and liquorice decoction fingerprint spectrum, acetonitrile is used as a mobile phase A, 0.05%-0.3% of phosphoric acid solution is used as a mobile phase B, and under the elution condition, the elution time is 30-60 min; the method has the advantages that the defect that the quality of relevant dosage forms of the Chinese herbaceous peony and liquorice decoction cannot be comprehensively and clearly detected due to interference caused by complex chemical components is overcome, 15 common characteristic peaks are obtained, effective separation of the 15 common characteristic peaks is realized, the peak shape is good and interference is avoided, so that relatively comprehensive and clear spectrum information is realized, and the detection accuracy is improved. Effective quality detection is carried out on the paeonia lactiflora and liquorice decoction, control over the quality of the paeonia lactiflora and liquorice decoction is further achieved, and the quality detection method has the advantages of being high in stability, high in precision, good in repeatability and the like.

Description

technical field [0001] The invention belongs to the field of drug analysis, and in particular relates to a Shaoyao Gancao Decoction fingerprint, a construction method thereof, and a detection method of a Shaoyao Gancao Decoction product. Background technique [0002] Shaoyao Gancao Decoction is from "Treatise on Febrile Diseases" (Han Zhang Zhongjing). Indication of body fluid damage, deficiency of yin blood, and various syndromes caused by the loss of tendons and meridians. The drug Jane Leehom can be used to treat a variety of diseases by adding and subtracting according to the symptoms. It has a wide range of clinical applications. When applying it, it is usually added and subtracted appropriately. effect. [0003] The composition of traditional Chinese medicine is complex, and the effective part is often not a single component. Using a single component as a quality control indicator has become more and more unsuitable for the quality control of traditional Chinese medi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/86G01N30/88G01N30/90
CPCG01N30/8686G01N30/88G01N30/90G01N2030/8809
Inventor 不公告发明人
Owner TONG REN TANG TECH CO LTD
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