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Caenorhabditis elegans particle food and preparation method thereof

A technology of C. elegans and food, applied in the field of C. elegans particulate food and its preparation, can solve the problems of low efficiency, metabolic changes, slow development, etc., and achieve the effect of simple equipment, normal growth and development, and intact organs

Pending Publication Date: 2022-07-05
SOUTH CHINA UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It can be seen that the nutritional components of the existing sterile culture solution cannot be fully retained in the body through the filter-feeding effect of Caenorhabditis elegans, so the efficiency of this nutrient supply method is relatively low
In fact, compared with C. elegans fed live bacteria, especially on solid media, C. elegans grown in existing sterile media did exhibit slower development, altered metabolism, etc.

Method used

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  • Caenorhabditis elegans particle food and preparation method thereof
  • Caenorhabditis elegans particle food and preparation method thereof
  • Caenorhabditis elegans particle food and preparation method thereof

Examples

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Embodiment 1

[0049] Example 1 Preparation of Caenorhabditis elegans particulate food NemaFood

[0050] The Caenorhabditis elegans particulate food NemaFood provided in this example can be processed by ultrasonic methods (such as high-energy ultrasonics, etc.), homogenization methods (such as high-pressure homogenization, etc.), cross-linking methods (such as physical cross-linking, chemical cross-linking, etc.) It is obtained by micronization of bacterial culture solution or inactivated bacteria. The preparation process of C. elegans granular food NemaFood is exemplified below by micronizing the sterile culture broth AXM by chemical cross-linking.

[0051] A preparation method of Caenorhabditis elegans granular food NemaFood, comprising the following steps: measuring 9 mL of sterile culture liquid AXM in a 50 mL conical flask, adding 3 mL of a 4 mg / mL sodium alginate solution, and manually stirring until uniform; Under the condition of ultrasonic at 400W, drop 4 mL of CaCl into the conica...

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Abstract

The invention belongs to the technical field of biology, discloses a caenorhabditis elegans microparticle food and a preparation method thereof, and particularly discloses a preparation method of the microparticle food, which comprises the following steps: mixing a caenorhabditis elegans sterile culture solution or inactivated bacteria with a macromolecular substance, and carrying out micronization treatment to obtain the microparticle food. According to the preparation method of the caenorhabditis elegans particle food, the caenorhabditis elegans sterile culture solution or inactivated bacteria are micronized, the formed submicron particles are good in embedding and dispersing effect, the preparation method is easy to implement, reaction conditions are mild, and needed equipment is simple.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a Caenorhabditis elegans particulate food and a preparation method thereof. Background technique [0002] Caenorhabditis elegans (Caenorhabditis elegans, hereinafter referred to as "C. elegans") is a tiny multicellular animal. As one of the most important model organisms in the field of modern biology, C. elegans has the advantages of short life cycle, small size, easy reproduction, and easy operation. It is widely used in cell biology, molecular biology, genetics, neuroscience and other disciplines. Research in various biomedical application fields such as food, pharmacy, and environment. In the daily operation of the laboratory, C. elegans is usually fed with a single live bacteria as food. The commonly used bacteria are Escherichia coli OP50, NA22 and other strains. Although these strains and feeding methods can better meet the general daily experiments, when the experimental wo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/00A23K20/163A23K40/00A23K10/16A23K20/147A23K20/20A01K67/033
CPCA23K50/00A23K20/163A23K40/00A23K10/16A23K20/147A23K20/20A01K67/033Y02A50/30
Inventor 黄泽波肖月王雨晴
Owner SOUTH CHINA UNIV OF TECH
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