Degradable microspheres simultaneously loaded with anti-cancer drug and active factor and provided with micro-nano structure, as well as preparation method and application of degradable microspheres

A micro-nano structure and active factor technology, applied in the field of biomedical materials, can solve the problems of not specifying the specificity of the morphology and structure of microspheres and nanoparticles, and not listing the results of cell experiments, so as to improve the quality of life and slow down the diffusion. , the effect of reducing side effects

Inactive Publication Date: 2020-03-17
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the patent also mentions enhancing cell adhesion, it does not list the actual cell experiment results
In addition, the patent does not specify the specificity of the morphology and structure of the microspheres and nanoparticles used

Method used

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  • Degradable microspheres simultaneously loaded with anti-cancer drug and active factor and provided with micro-nano structure, as well as preparation method and application of degradable microspheres
  • Degradable microspheres simultaneously loaded with anti-cancer drug and active factor and provided with micro-nano structure, as well as preparation method and application of degradable microspheres
  • Degradable microspheres simultaneously loaded with anti-cancer drug and active factor and provided with micro-nano structure, as well as preparation method and application of degradable microspheres

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Experimental program
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preparation example Construction

[0028] The present invention firstly provides a method for preparing degradable microspheres with micro-nano structure simultaneously loaded with anticancer drugs and active factors, comprising the following steps:

[0029] Step 1: using degradable polymers as raw materials to prepare polymer microspheres loaded with active factors; the degradable polymers include aliphatic polyester or polyester polyether copolymers;

[0030]Step 2: Using biomedical polymers as raw materials, prepare micro-nano particles loaded with anticancer drugs; the biomedical polymers include aliphatic polyesters, polyester polyether copolymers, polystyrene, polyacrylates, At least one of polymethacrylate, polyacrylonitrile or polyisobutylene;

[0031] Step 3: Composite polymer microspheres loaded with active factors and micro-nano particles loaded with anti-cancer drugs, so that micro-nano particles are loaded on the polymer microspheres, and a micro-nano structure loaded with anti-cancer drugs and act...

Embodiment 1

[0052] Example 1 Preparation of micro-nano structured porous PLGA microspheres loaded with paclitaxel and RGD

[0053] Prepare 2.5 mL of 10% (g / mL) PCL / CHCl 3 solution, then add 100 μg paclitaxel, above-mentioned solution and 5ml, 0.5% (g / mL) PVA aqueous solution are ultrasonic 1min under 200w to obtain emulsion, above-mentioned emulsion is added in the PVA aqueous solution of 100ml 0.5% (g / mL), under 700rpm Continue to stir for 5h until CHCl 3 After volatilization, the paclitaxel-embedded PCL micro-nanoparticles were obtained, washed and collected. The prepared PCL nanoparticles were further soaked in excess 0.1M NaOH solution for 30 min, washed and filtered repeatedly to remove excess NaOH on the surface of the nanoparticles.

[0054] Preparation of PLGA / CH 2 Cl 2 The solution is used as the oil phase (O), the solution is placed under the homogenizer and immersed in an ice-water bath to maintain the temperature, and the prepared inner water phase (W) is added to the oil ...

Embodiment 2

[0056] Example 2 Preparation of micro-nano structured PCL-PEG microspheres loaded with camptothecin and REDV

[0057] First prepare 2.5mL, 10% (g / mL) of PCL-PEG-NH 2 / CHCl 3 Solution, then add 100 μ g camptothecin, above-mentioned solution and 5ml, the PVA aqueous solution of 0.5% (g / mL) obtain emulsion under 200w supersonic 1min, above-mentioned emulsion is added in the PVA aqueous solution of 100ml 0.5% (g / mL), Continue stirring at 700rpm for 5h until CHCl 3 Get PCL-PEG-NH after volatilization 2 Micro-nanoparticles, washed and collected.

[0058] Preparation of PCL-PEG-COOH / CH 2 Cl 2 The solution is used as the oil phase (O), the solution is placed under the ultrasonic probe and immersed in an ice-water bath to maintain the temperature, and the prepared internal water phase (W) is injected into the oil phase. 1 ), and obtain emulsion (E 1 ), then E at room temperature 1 Add dropwise to the external aqueous phase (W 2 ), continue to stir for 2h after the dropwise add...

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Abstract

The invention provides degradable microspheres simultaneously loaded with an anti-cancer drug and an active factor and provided with a micro-nano structure, as well as a preparation method and application of the degradable microspheres and belongs to the technical field of biomedical materials. According to the method, polymer microspheres loaded with an active factor are prepared with a degradable polymer adopted as a raw material; micro-nano particles loaded with an anti-cancer drug are prepared with biomedical macromolecules adopted as a raw material; and the polymer microspheres loaded with the active factor and the micro-nano particles loaded with the anti-cancer drug are compounded, so that the degradable microspheres simultaneously loaded with the anti-cancer drug and the active factor and provided with the micro-nano structure can be obtained. According to the method of the invention, the micro-nano particles are used for modifying the degradable microspheres, so that the growth of cells and tissues on the microspheres is improved through the micro-nano topological morphology of the surfaces of the microspheres; through the differential and sequential controllable releaseof the drug and active factor by the microspheres and the micro-nano particles, the lasting synergistic treatment effect of the degradable microspheres can be fully exerted; and the degradable microspheres are of great significance to the treatment and repair of defective tissues and organs.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and specifically relates to a degradable microsphere with a micro-nano structure loaded with anticancer drugs and active factors, and a preparation method and application thereof. Background technique [0002] At present, malignant tumors (commonly known as cancer) have become the second leading cause of death after cardiovascular diseases in the world. Surgical treatment of cancer, that is, directly removing tumors through surgery is a commonly used method of cancer treatment in clinic, and it is one of the most important treatment methods for early and mid-stage cancer patients. However, this method has certain limitations, because it is sometimes difficult or even impossible to remove the tumor tissue completely due to the limitation of the invasive growth of cancer cells and the special location of tumor growth. In addition, most operations not only remove the tumor itself, but ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K45/06A61K47/34A61K47/69A61L27/18A61L27/50A61L27/54A61L27/58A61P35/00
CPCA61K9/5153A61K45/06A61K47/6935A61L27/18A61L27/50A61L27/54A61L27/58A61L2300/416A61L2300/604A61L2300/624A61P35/00
Inventor 石旭东庄秀丽甘志华陈学思王淑英孙海崔立国
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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