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Acute sepsis non-human primate model and construction method thereof

A technology of primate and construction method, which is applied in the field of acute sepsis non-human primate model and its construction, can solve the problems of unfavorable animal protection and welfare, and can not simulate human sepsis well, to achieve The effect of stable model results and simple methods

Pending Publication Date: 2022-07-15
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In summary, the genetic background differences between rodents and humans cannot well simulate the occurrence and development of human sepsis, and in the application of the model, the number of animals consumed by rodents in conventional blood collection methods for pharmacokinetic studies is relatively small. Large, detrimental to animal welfare

Method used

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  • Acute sepsis non-human primate model and construction method thereof
  • Acute sepsis non-human primate model and construction method thereof
  • Acute sepsis non-human primate model and construction method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0041] In this experiment, 18 adult cynomolgus monkeys aged 8-16 were selected and divided into four groups: A, B, C, D, and were fed with normal feed in a single cage and adapted to feeding for 7 days. During the adaptive feeding of cynomolgus monkeys, the standard strain of Escherichia coli ATCC25922 was aerobically cultured on nutrient agar medium at 37°C for 18 hours, and the bacterial solution was diluted to 5×10^5 cells / mL. The cynomolgus monkeys were fasted at night and weighed from 7:30-8:00 am the next morning. The cynomolgus monkeys were weighed and restrained, the inner side of the left arm was exposed upward, the hair was removed with an animal shaver, and the vein was filled by wiping with an alcohol cotton ball. The cynomolgus monkeys divided into four groups A, B, C, D were injected with Escherichia coli intravenously at 0.5mL / kg, 1.0mL / kg, 1.5mL / kg, 3.0mL / kg, parallel injection, and bolus injection of bacteria. Use dry cotton balls to stop the bleeding. The do...

Embodiment 2

[0045] A method for constructing a non-human primate model of acute sepsis, comprising the following steps:

[0046] In this experiment, 6 adult cynomolgus monkeys aged 12-18 were selected and fed with normal feed in a single cage and adapted to feeding for 7 days. During the adaptive feeding of cynomolgus monkeys, the standard strain of Escherichia coli ATCC25922 was aerobically cultured on nutrient agar medium at 37°C for 18 hours, and the bacterial solution was diluted to 6.3×10^3 / mL. The cynomolgus monkeys were fasted at night and weighed from 7:30-8:00 am the next morning. The cynomolgus monkeys were weighed and restrained, the inner side of the left arm was exposed upward, the hair was removed with an animal shaver, and the vein was filled by wiping with an alcohol cotton ball. Escherichia coli was injected intravenously at 1 mL / kg, and the needle was inserted in parallel. After bolus injection of bacterial solution, the bleeding was stopped by pressing with a dry cotto...

Embodiment 3

[0050] A method for constructing a non-human primate model of acute sepsis, comprising the following steps:

[0051] In this experiment, 6 adult cynomolgus monkeys aged 15-20 years were selected and fed with normal feed in a single cage and adapted to feeding for 7 days. During the adaptive feeding of cynomolgus monkeys, the standard strain of Escherichia coli ATCC25922 was aerobically cultured on nutrient agar medium at 37°C for 18 hours, and the bacterial solution was diluted to 6.3×10^5 cells / mL. The cynomolgus monkeys were fasted at night and weighed from 7:30-8:00 am the next morning. The cynomolgus monkeys were weighed and restrained, the inner side of the left arm was exposed upward, the hair was removed with an animal shaver, and the vein was filled by wiping with an alcohol cotton ball. Escherichia coli was injected intravenously at 1 mL / kg, and the needle was inserted in parallel. After bolus injection of the bacterial solution, the bleeding was stopped by pressing ...

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Abstract

The invention discloses an acute sepsis non-human primate model and a construction method thereof, and belongs to the field of infection experiment model construction. The construction method comprises the following steps: by taking a non-human primate animal, namely cynomolgus monkey, as a model animal, carrying out intravenous injection of escherichia coli, so that the inflammation immune indexes such as blood C-reactive protein, procalcitonin and leukocyte quantity of the cynomolgus monkey within 120 hours are remarkably improved; the increase of glutamic-pyruvic transaminase and glutamic oxalacetic transaminase, the reduction of albumin level and the like reflect the impaired liver function, and the prolonging of activated partial thrombin time, the reduction of prothrombin activity and the like reflect the blood coagulation dysfunction, so that acute sepsis is caused. Compared with a model constructed by taking a rodent as a model animal, the acute sepsis non-human primate model constructed by the invention can better simulate related symptoms and physiological processes of human sepsis, and a preclinical effect evaluation tool is provided for developing research and development of nutrients and medicines in related fields.

Description

technical field [0001] The invention belongs to the field of infection experimental model construction, in particular to a non-human primate model of acute sepsis and a construction method thereof. Background technique [0002] Sepsis is a process in which pathogenic microorganisms such as bacteria invade the body and induce systemic inflammatory responses or immune disorders in the host. Sepsis can lead to the activation of the body's coagulation system and the imbalance of immune response, mainly manifested as decreased plasma protein concentration, decreased peripheral platelet count, increased neutrophil counts, etc. The most significant change is serum C-reactive protein (C-reactive protein). , CRP) and procalcitonin (procalcitonin, PCT) levels increased. In addition to the significant changes in blood indicators, severe sepsis can cause changes in the function of multiple organs in the body, which is dangerous and expensive to treat. [0003] In recent years, despite...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02
CPCA01K67/02Y02A50/30
Inventor 任娇艳刘炜徐真真
Owner SOUTH CHINA UNIV OF TECH
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