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Application of mesenchymal stem cell exosome

A technology of bone marrow mesenchymal and stem cells, applied in the fields of biology and medicine, to achieve the effect of reducing liver ischemia-reperfusion injury

Pending Publication Date: 2022-07-15
GUILIN MEDICAL UNIVERSITY
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Problems solved by technology

Studies have shown that exosomes from mesenchymal stem cells can regulate apoptosis and reduce hepatic ischemia-reperfusion injury. The mechanism needs to be further studied

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  • Application of mesenchymal stem cell exosome
  • Application of mesenchymal stem cell exosome
  • Application of mesenchymal stem cell exosome

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Embodiment Construction

[0028] The present invention will be further described below with reference to the accompanying drawings and specific embodiments. It should be noted that, on the premise of no conflict, the embodiments or technical features described below can be combined arbitrarily to form new embodiments. . In the following examples, unless otherwise indicated, all reagents used are of analytical grade, and all reagents used can be obtained from commercial sources. The experimental methods for which specific conditions are not indicated in the text are usually in accordance with conventional conditions, such as those described in the book "Molecular Cloning Experiment Guide" published by Science Press, edited by J. Sambrook et al. in 2002, or as suggested by the manufacturer. conditions of. Unless otherwise defined, all professional and scientific terms used herein have the same meanings as those familiar to those skilled in the art. In addition, any methods and materials similar or equi...

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Abstract

The invention provides an application of a bone marrow mesenchymal stem cell exosome. After the BMSCs-Exo is injected into the HIRI Kunming mouse, the liver function impairment can be relieved. Wherein the miR-25-3p is obviously reduced in an HIRI liver, the BMSCs and the BMSCs-Exo both contain the miR-25-3p, after the miR-25-3p mimics and the miR-25-3p mimic are respectively transferred into an HIRI mouse and an anoxic / reoxygenation AML12 hepatocyte, the expression quantity of the miR-25-3p is increased, the expression of apoptosis and inflammation related factors is reduced through qRT-PCR (quantitative reverse transcription-polymerase chain reaction) and Western blotting detection, and the apoptosis and inflammation related factors are inhibited. Moreover, by measuring the contents of aspartate transaminase (AST) and alanine transaminase (ALT) in serum and hematoxylin-eosin (HE) staining, the liver injury is reduced. In addition, the expression of genes and related proteins of the MAPK / ERK signal channel and the PI3K / AKT signal channel detected by qRT-PCR (quantitative real-time polymerase chain reaction) and Western blotting is reduced. Therefore, the miR-25-3p inhibits hepatocyte apoptosis induced by HIRI injury by inhibiting MAPK / ERK and PI3K / AKT signal channels, so that the HIRI is improved, and a new thought is provided for treatment of the HIRI.

Description

technical field [0001] The present invention relates to the fields of biology and medicine, in particular to the application of bone marrow mesenchymal stem cell exosomes. Background technique [0002] Hepatic ischemia-reperfusion injury (HIRI) refers to a pathological phenomenon in which the blood supply to the liver is blocked for a period of time during liver surgery, and the blood flow is restored. . HIRI is a common clinical problem in the management of liver trauma, hepatic lobectomy, and liver transplantation. It can lead to organ failure, increase the incidence of rejection, and have a great impact on the prognosis and survival of patients. So far, there is still a lack of clinically effective interventions. During the ischemia-reperfusion process, cells are in a state of oxidative stress, endoplasmic reticulum stress and calcium overload, mitochondrial swelling and outer membrane rupture, cytochrome C release, thereby triggering apoptosis signaling pathways, leadi...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61P1/16
CPCA61K35/28A61P1/16
Inventor 邢雪琨李红男刘润生张佳阳亢小敏林柯庄王瑞华陈晓琳牙海妮江俞臻
Owner GUILIN MEDICAL UNIVERSITY
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