Bronchoalveolar lavage fluid obtained for in-vitro lung and obtaining method of bronchoalveolar lavage fluid

Abstract

The invention relates to the technical field of bronchoalveolar lavage, and aims to provide in-vitro lung bronchoalveolar lavage fluid and an acquisition method thereof. The bronchoalveolar lavage fluid obtained for the in-vitro lung contains rich biological information, and the obtaining method is harmless to a patient and free of danger and meets ethical requirements.

Description

technical field [0001] The invention relates to the technical field of bronchoalveolar lavage fluid, and more particularly, to a bronchoalveolar lavage fluid for isolated lungs and a method for obtaining the same. Background technique [0002] The traditional method of obtaining bronchoalveolar lavage fluid (BALF) is through the application of fiberoptic bronchoscopy. The main steps are: inject normal saline at room temperature into the lobe segment bronchus under direct vision through a bronchoscope, stay for a few seconds, and then withdraw the perfusate after the perfusate flows to the alveoli. Alveoli can theoretically collect sub-bronchial lung segments, sub-pulmonary segments and alveolar surface lining fluid, which is BALF. Laboratory detection of BALF can provide important reference value for clinical diagnosis, differential diagnosis, treatment effect evaluation and prognosis. In addition, BALF contains a large number of human cells, a variety of microorganisms, c...

AI Technical Summary

Problems solved by technology

Ordinary spit and deep sputum basically do not contain the biological information carried by the lining fluid on the surface of the bronchioles and alveoli, so neither can replace BALF

Moreover, patients with lung diseases such as interstitial lung disease and pulmonary hypertension generally have no sputum, and cannot obtain lung biological information through sputum

[0003] However, the current method of obtaining BALF is recovery by bronchoscopic lavage, which has the following disadvantages: 1) This operation is invasive and has certain risks, which may cause allergy to anesthetic drugs, fever, cough, glottic edema, Bronchospasm, tachycardia, elevated blood pressure, tracheal mucosal hemorrhage, pneumothorax and other related complications; for patients with end-stage lung disease, these complications may lead to further aggravation of respiratory failure, and may even cause death; 2) as an effective invasive examination, all patients with end-stage lung disease have respiratory failure, extremely poor oxygen reserves, and generally cannot tolerate bronchoscopy and endoscopic lavage; 3) This examination method has certain contraindications, such as severe cough, severe respiratory failure, Patients with moderate to severe pulmonary hypertension, coagulation disorders, severe thrombocytopenia, and severe cardiac insufficiency cannot accept this test

[0004] In addition, the acquisition of BLAF by bronchoscopy has the following disadvantages: 1) the amount of lavage fluid is small, and each lavage fluid is only 10ml; 2) the recovery rate is low, about 5ml of BALF is obtained, and the recovery rate is only 40-60%; 3) The lavage site is not accurate enough to selectively lavage the target site through microscopic hematuria; 4) The ratio of deep alveolar surface fluid is low, and the biological information carried cannot fully reflect the deep alveolar tissue; 5) The frequency of lavage must be determined according to the patient’s Situation adjustments, unstable acquisitions, etc.

Therefore, the traditional BALF and its acquisition method cannot meet the needs of clinical and scientific research, and a new bronchoalveolar lavage fluid and its acquisition method are urgently needed to be invented

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