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Gracilaria lemaneiformis polypeptide with antihypertensive activity, preparation method and application

An asparagus, active technology, applied in the field of peptides, to achieve the effect of strong interaction and high activity

Active Publication Date: 2022-07-22
FISHERIES RES INST OF FUJIAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Currently, there are technologies for preparing polypeptides with antihypertensive activity from foods such as hazelnuts, yak milk, shellfish, and wolfberry, but there is no technology for preparing polypeptides with antihypertensive activities using asparagus as a raw material

Method used

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  • Gracilaria lemaneiformis polypeptide with antihypertensive activity, preparation method and application
  • Gracilaria lemaneiformis polypeptide with antihypertensive activity, preparation method and application
  • Gracilaria lemaneiformis polypeptide with antihypertensive activity, preparation method and application

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Embodiment Construction

[0024] The present invention will now be further described with reference to the accompanying drawings and specific embodiments.

[0025] The amino acid sequence of the Astragalus polypeptide with antihypertensive activity provided in this example is: RLVPVPY, its molecular weight is 842.5014Da, and its IC inhibits ACE. 5O The value was 6.45 μmol / L.

[0026] Study on the structure-activity relationship of the Polypeptides of Astragalus with antihypertensive activity:

[0027] Methods: Use AutoDock to do molecular docking between the ACE-C-terminal domain and A. mustard polypeptide with antihypertensive activity, and set the flexibility of the ligand of A. mustardii polypeptide with antihypertensive activity. Other parameters are defaulted, and Vina program is used for molecular docking. To analyze the structure-activity relationship between the polypeptides with antihypertensive activity and ACE.

[0028] Result: as image 3 As shown, RLVPVPY forms hydrogen bonding interact...

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Abstract

The invention discloses gracilaria lemaneiformis polypeptide with antihypertensive activity, a preparation method and application, and belongs to the technical field of polypeptides, and the amino acid sequence of the gracilaria lemaneiformis polypeptide with antihypertensive activity is RLVPVPY. The preparation method comprises the following steps: S1, carrying out enzymolysis and filtration treatment on gracilaria lemaneiformis to obtain clear polypeptide enzymatic hydrolysate, S2, carrying out ultrafiltration treatment, respectively collecting polypeptide components with different molecular weights, and freeze-drying to obtain gracilaria lemaneiformis polypeptide freeze-dried powder, S3, carrying out mass spectrum determination and analysis to obtain a plurality of polypeptide sequences, S4, screening out polypeptides of which the docking scores are ranked in the top, and S5, screening out polypeptides of which the docking scores are ranked in the top. And S5, selecting the polypeptide with the ACE inhibition ratio of more than 80%, so as to obtain the asparagus polypeptide with the antihypertensive activity. Gracilaria lemaneiformis is used as a raw material, the amino acid sequence of the gracilaria lemaneiformis polypeptide with the antihypertensive activity is obtained, and the prepared gracilaria lemaneiformis polypeptide with the antihypertensive activity has good ACE inhibitory activity.

Description

technical field [0001] The invention belongs to the technical field of polypeptides, and in particular relates to a mustard polypeptide with antihypertensive activity, a preparation method and an application. Background technique [0002] Astragalus belongs to the red algae family, Gracilaria, and the genus Jiangli, also known as seaweed or seaweed, is the third largest marine algae in the world. Its high yield (the second in China), low cost, wide range of nurturing, and high economic value have attracted much attention. Its body is rich in polysaccharide, protein, crude fiber and other nutrient resources. It is a natural food for diet and medicine. It is often used to develop and utilize various biologically active substances such as polysaccharides, polypeptides, phycoerythrin, dietary fiber, etc. Therefore, astragalus has potential development value in the fields of marine food, food additives, functional food, marine medicine and so on. [0003] Angiotensin-converting...

Claims

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Application Information

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IPC IPC(8): C07K7/06C12P21/06C07K1/34C07K1/14A61K38/08A61P9/12
CPCC07K7/06C12P21/06A61P9/12A61K38/00Y02A50/30
Inventor 刘智禹苏永昌刘淑集陈贝乔琨许旻陈晓婷
Owner FISHERIES RES INST OF FUJIAN
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