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Sea cucumber viscera antihypertensive active peptide and preparation method thereof

A technology of sea cucumber viscera and active peptides, which is applied in the preparation methods of peptides, chemical instruments and methods, peptides, etc., can solve the problems of no antihypertensive activity in the body, and the active peptides are not resistant to digestion, and achieves no toxic side effects and high safety. , the effect of stable antihypertensive effect

Active Publication Date: 2016-05-18
肽牛(北京)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0017] The invention mainly solves the problem that the existing active peptides are not resistant to digestion and basically have no antihypertensive activity in vivo

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1, weigh 100g of Icelandic sea cucumber inner wall and sea cucumber sausage mixture, rinse to remove sediment impurities, remove after soaking, control clean water, add 200mL distilled water, chop, then put it into a tissue blender for homogenization ; Sterilize and thermally denature the homogenized slurry at a temperature of 80°C for 10 minutes; cool the viscera of the beaten sea cucumber, put it into a large beaker, stir and add neutral protease (5000U / g raw material), and Enzymolysis at constant temperature at 45°C for 9 hours; after the enzymolysis is completed, take out the hydrolyzate and boil water to inactivate the enzyme for 10 minutes, then filter it to obtain the supernatant after cooling; the supernatant is passed through ultrafiltration membranes with different molecular weights, and the filtrate is collected , the ultrafiltration membrane to be used has a molecular weight cut-off of 5kDa; then for debittering, first add powdered activated carbon wi...

Embodiment 2

[0041] Example 2, weigh 100g of Icelandic sea cucumber's sea cucumber sausage, rinse to remove sediment impurities, remove after soaking, control clean water, add 200mL of distilled water, chop, and then put it into a tissue grinder for homogenization; after homogenization, Sterilization and heat denaturation treatment of the slurry, the treatment temperature is 60°C, and the time is 10 minutes; after the beaten sea cucumber intestines are cooled, put them into a large beaker, stir and add neutral protease (5000U / g raw material), and keep the temperature at 45°C Enzymolysis for 9 hours; after the enzymolysis is completed, take out the hydrolyzate and boil the water to inactivate the enzyme for 10 minutes, then filter it to obtain the supernatant after cooling; The filter membrane has a molecular weight cut-off of 1KDa; then it is debittered and masked with spices; it is made into a freeze-dried product according to a conventional process.

Embodiment 3

[0042] Embodiment 3, take by weighing the sea cucumber inner wall of 100g Icelandic sea cucumber, rinse to remove silt impurities, pull out after soaking, control the clean water, add 200mL distilled water, chop, then put it into a tissue grinder for homogenization; after homogenization Sterilization and heat denaturation treatment of the slurry, the treatment temperature is 100°C, and the time is 10 minutes; after cooling the beaten sea cucumber intestines, put them into a large beaker, stir and add neutral protease (5000U / g raw material), and keep the temperature at 45°C Enzymolysis for 9 hours; after the enzymolysis is completed, take out the hydrolyzate and boil the water to inactivate the enzyme for 10 minutes, then filter it to obtain the supernatant after cooling; The filter membrane has a molecular weight cut-off of 10KDa; then debitterize, add 1.5% β-cyclodextrin to the filtrate, and embed at 60°C for 60 minutes, or add 0.5% powdered activated carbon to the filtrate, a...

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PUM

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Abstract

The invention discloses a sea cucumber offal antihypertensive active peptide and a preparation method thereof which are characterized in that the active peptide is prepared by using the following method: washing and soaking sea cucumber offal, taking out the sea cucumber offal, draining off water, adding distilled water, cutting up the sea cucumber offal, and putting the sea cucumber offal into a tissue crusher to homogenize; sequentially carrying out sterilization and thermal denaturation treatment on slurry; after the slurry is cooled, putting the slurry into a large beaker, adding neutral protease while stirring, and carrying out constant-temperature enzymolysis; after the enzymolysis is completed, taking out hydrolysate, carrying out enzyme deactivation on the hydrolysate in boiling water for 10min, cooling, and then filtering so as to obtain liquid supernatant; enabling the liquid supernatant to pass through an ultrafiltration membrane, and collecting a filtered solution; carrying out debitterizing treatment on the filtered solution; preparing a product in an oral liquid form, a freeze-dried product form or a powder form after spray drying. The product is high in safety, has no toxic or side effect, has a stable antihypertensive effect, has the characteristics of versatility and easy absorption and has an anti-digestion effect, and the product does not produce three-waste pollution in the process of processing, therefore, the product is environmental-friendly.

Description

Technical field: [0001] The present invention relates to the technical field of sea cucumber viscera processing, specifically sea cucumber viscera antihypertensive active peptide and its preparation method. technical background: [0002] 1. The hazards and current situation of hypertension: Hypertension is a common cardiovascular disease with a high incidence and is often accompanied by functional or organic changes in the heart, blood vessels, lungs, and kidneys. , kidney and eye vessels and other complications and lead to stroke, promote atherosclerosis, an important risk factor for coronary heart disease. In the early stage of high blood pressure, there are generally no symptoms, until clinical symptoms such as heart attack and cerebrovascular rupture occur, so high blood pressure is also called the "silent killer"; statistics show that the incidence of high blood pressure in developed countries reaches more than 20% , the average incidence rate of countries in the world...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/34
Inventor 刘凯徐莲高莹孙姿姿魏玉良
Owner 肽牛(北京)生物科技有限公司
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