Method for reducing TSWV (Total Standard Wave Virus) virus carrying amount in adult thrips
A technology for thrips and adults, which is applied in the field of reducing the amount of TSWV in the body of adult thrips, can solve problems such as endangering human health and soil environment pollution, achieves resistance to pathogens' damage to crops, and reduces the risk of tomato spotted wilt virus Effect
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Embodiment 1
[0028] The preparation of prodigiosin sustained-release preparation, the steps are as follows:
[0029] Add 5mL of sodium alginate solution (0.1g / mL) into a 50mL conical flask, under magnetic stirring, add 4mL of isopropanol and 2mL of glutaraldehyde in turn, stir well and then stand still for 30min; then add 0.5mL of Tween- 20. 1mL prodigiosin solution (1g / mL) and 0.5mL salicylic acid solution (1g / mL), stir for 1h and mix well; centrifuge in a low temperature centrifuge at 4°C, 11000×g for 10min, remove the supernatant, Washing with deionized water three times to obtain prodigiosin nanocapsules, namely prodigiosin sustained-release preparation, which is stored in the dark for future use.
[0030] (1) Analysis of physical properties
[0031] The particle size, Zeta potential and PDI data of prodigiosin sustained-release preparation were detected, and the results were as follows figure 1 and figure 2 As shown, the average particle size of the prodigiosin sustained-release p...
Embodiment 2
[0036] To test the poisoning ability of Western flower thrips, the steps are as follows:
[0037] The healthy and non-toxic Nicotiana benthamiana was cultivated in flower pots and used for the test when it grew to 5-6 true leaves. Put 1 g of tobacco leaves with TSWV into a pre-sterilized mortar, add 10 mL of 1×PBS buffer, and grind as an inoculum. Take the healthy N. benthamiana plants to be inoculated, sprinkle a small amount of 500-mesh emery powder on the top 2-3 true leaves, then use a cotton swab to dip an appropriate amount of inoculum on the leaf veins and gently rub the inoculation, and rinse gently with water after 30 minutes Leaves were placed in a dark environment for 24 hours and then transferred to a normal environment to continue culturing. About 7-10 days after inoculation, typical symptoms such as deformity, chlorosis, yellowing, and mottled appear, such as Figure 5 As shown, it is the virus-carrying plant, which is used for subsequent experiments.
[0038]...
Embodiment 3
[0044] To test the virus transmission ability of Western flower thrips, the steps are as follows:
[0045] Get the adult worms raised by two treatments in the embodiment 2 as the test worms, after 5 days of eclosion, adopt the live N. benthamiana to detect its poison transmission ability, and the concrete method is: the N. benthamiana seedlings are transplanted to a height of 35cm, and the diameter is a plastic plastic of 20cm. In the barrel (the thickness of the plant medium at the bottom of the barrel is about 10 cm), the plastic barrel is covered with a 200-mesh insect-proof gauze. When N. benthamiana cultivated to 4-5 true leaves, the test was divided into three treatments, treatment a: each barrel was fed with a female or male adult worm raised in the treatment in Example 2 above; treatment b: each barrel was fed with the above-mentioned In example 2, handle two reared female adults or one male adult; process c: do not access test worms, as control (CK); each treatment re...
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