MiR-181a-5p nanocomposite for treating oral cancer as well as preparation method and application of miR-181a-5p nanocomposite

Abstract

The invention provides a miR-181a-5p nano compound for treating oral cancer as well as a preparation method and application of the miR-181a-5p nano compound. The polyethyleneimine modified silver nanoparticles are selected, a culture medium without double antibodies is generally needed during cell transfection, and nano-silver has a certain bacteriostatic effect and can effectively reduce the risk that cells are polluted during transfection, so that the operation is simpler, more convenient, safer and more effective when the nano-carrier is used; and the experiment efficiency can be improved. Besides, the polyethyleneimine modified nano-silver compound has certain pH responsiveness, the targeting of nano-drugs to tumor sites and the release efficiency of gene sequences can be remarkably improved, and research on in-vivo and in-vitro anti-tumor mechanisms is more convenient. The nano-carrier is used for delivering miR-181a-5p with an oral cancer inhibition function, and the expression quantity of miR-181a-5p in cells can be improved while the serum stability and RNA enzyme stability of mimics of miR-181a-5p can be remarkably improved, so that malignant behaviors such as proliferation, migration and invasion of oral cancer cells are effectively inhibited.

Description

technical field [0001] The invention belongs to the field of medicine; in particular, it relates to a miR-181a-5p nanocomposite for treating oral cancer, a preparation method and an application. Background technique [0002] Oral cancer is a global malignancy; 400,000 cancer-related deaths each year are caused by oral cancer. At the same time, the incidence of oral squamous cell carcinoma is also increasing year by year, and it tends to be younger year by year. In recent years, although clinical treatment and diagnostic techniques have made great progress, the 5-year survival rate and quality of life of patients have not been significantly improved. At the same time, the clinical treatment of oral cancer will still seriously affect the patient's language expression, chewing and swallowing, and may also cause functional diseases such as dental health and social interaction deficits, and even seriously affect the overall image of the patient's face. The neglect of oral hygie...

AI Technical Summary

Problems solved by technology

[0009] Preliminary results have fully demonstrated that miR-181a-5p may play the role of a tumor suppressor gene in oral cancer. Although a variety of techniques have been used to verify the relationship between miRNA and various tumors, there is still a lack of miR-181a-5p in oral cancer. Involved in the regulation of signaling pathways in oral squamous cell carcinoma, the specific regulatory mechanism, and whether miR-181a-5p can play a dual role in drug therapy and gene therapy by combining miR-181a-5p with other new anticancer drugs

At the same time, there are still many problems in the actual use of miRNA in clinical treatment. The traditional liposome transfection method has the disadvantages of high toxicity, low efficiency, no specificity and targeting effect, and difficult transfection in vivo. Seriously hindering the clinical application of miRNA-targeted therapy

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