Anti-glycated collagen tetrapeptide (PGXR) containing specific sequence and preparation method thereof
A collagen peptide and strain technology, applied in the biological field, can solve the problems of industrial production impact, lack of better technology disclosure, etc., and achieve the effects of good anti-glycation ability and good market prospects.
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Embodiment 1
[0056] Example 1 Preparation of anti-glycation vascular collagen peptides
[0057] In this example, alkaline protease (Bacillus licheniformis protease), bromelain, and flavor enzyme were all purchased from Nanning Dongheng Huadao Biotechnology Co., Ltd.
[0058]In the present embodiment, Lactobacillus plantarum was purchased from Ningbo Testo Biotechnology Co., Ltd., serial number: TS279068; Lactobacillus paracasei was purchased from Chonghui (Beijing) Biotechnology Co., Ltd., product number CHCC345679; Staphylococcus saccharides was purchased from Shanghai Yiyan Biotechnology Co., Ltd., item number EY09738J.
[0059] Weigh 100 g of tilapia skin and cut it into small pieces, add 2000 mL of 5% sodium bicarbonate solution to soak for 4 hours, wash 3 times, add an appropriate amount of demineralized water, and homogenize it in a grinder to a paste; adjust the pH to 2.5, Heated to 90 ℃, thermal insulation for 6h. After filtration through the mesh, adjust the pH to 8.0, the tempe...
Embodiment 2
[0062] Example 2 Evaluation of anti-glycation ability of collagen peptides
[0063] In this example, the anti-glycation collagen peptide obtained in Example 1 was tested according to the evaluation method of anti-glycation ability, and the commercially available collagen peptide was used as a control. The commercially available collagen peptide was provided by Beijing Shengminuo Biotechnology Co., Ltd.
[0064] The specific evaluation method of anti-glycation ability is: using BSA-fructose simulation reaction system, mix 1mL fructose solution (1.5mol / L) with 1mL collagen peptide component solution, incubate at 37°C for 2h, add 1mL 30mg / mL BSA solution, the above The reactants were dissolved in 50mmol / L pH7.4 phosphate buffer (containing 0.1% sodium azide), and the aminoguanidine solution of the same mass concentration was used to replace each component solution of collagen peptide as a positive control group, and phosphate buffer was used. Instead of each component solution of...
Embodiment 3
[0069] The experiment was carried out with reference to the method of Example 1. The difference was that the combined enzymes included 0.4g alkaline protease, 0.3g bromelain, and 0.3g flavor enzyme, and the test result of the final product's anti-glycation ability was 83%.
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