Application of 6-phosphoric acid-trehalose and cultivation method for improving yield and disease resistance of common kidney beans
A cultivation method, trehalose technology, applied in the field of crop cultivation, can solve problems affecting crop growth, threatening food security, yield loss, etc., and achieve the effects of promoting plant growth, improving yield and resistance level, and increasing sugar content
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[0017] Common kidney bean seedlings were treated by spray method, and distilled water was used as solvent to prepare 1mM 6-phosphoric acid-trehalose solution (T6P).
Embodiment 2
[0019] 7-day-old kidney bean seedlings were prepared, sprayed with 1 mM T6P once a day, and continuously treated for 7 days for inoculation of pathogenic bacteria. The inoculation method was referred to Xue Renfeng et al. (2018).
[0020] The brief description is as follows: a mixture of corn flour and vermiculite is prepared at a ratio of 1:2 (V / V), and 400 mL of the mixture is taken into a 1000 mL conical flask, sterilized twice at 121°C under 0.2MP for 30 minutes. Add 50 mL of sterilized distilled water to the corn flour mixture, mix it evenly, and then add a dish of pathogenic bacteria, culture at 25-28°C for 7-10 days, and shake the triangular flask every day to make the pathogenic bacteria grow evenly. Mix the inoculum with the sterilized nutrient soil at a ratio of 1:10, take 0.01 g of the inoculated mixed soil and suspend it in 1 mL of sterilized water, and use a hemocytometer to calculate the content of pathogenic bacteria in the inoculated mixed soil, so that the fina...
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