Application of PERK in prevention or treatment of female reproductive dysfunction or ovarian lesion

A reproductive function and disorder technology, applied in the direction of disease, microbial measurement/test, medical preparations containing active ingredients, etc., can solve problems such as increased apoptosis, damage to reproductive cells, and decreased vitality

Pending Publication Date: 2022-08-09
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, experiments have confirmed that low temperature has a rapid interference effect on spermatogenesis and sperm motility. After cooling, the oxygen activity in the testis of rats is significantly reduced, and the hypoxia of testicular tissue has an impact on the synthesis and activity of proteins and enzymes, thereby affecting sperm production. Formation and maturation, enhanced peroxidation of germ cell membrane lipids,...

Method used

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  • Application of PERK in prevention or treatment of female reproductive dysfunction or ovarian lesion
  • Application of PERK in prevention or treatment of female reproductive dysfunction or ovarian lesion
  • Application of PERK in prevention or treatment of female reproductive dysfunction or ovarian lesion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1 Effects of cold exposure on reproductive cycle and ovarian follicle development

[0062] 8-week-old unmated female mice were used to establish intermittent cold exposure experimental animal models (10:00-14:00, continuously exposed to 4°C for 4 hours per day, for 3 consecutive days), and the model mice and control group mice were used for the rest of the time. They were also raised under normal temperature (22°C), and they were allowed to eat and drink freely.

[0063] After continuous intermittent cold exposure of the mice in the experimental group for 3 days, the vaginal cytology smear method was used to detect the animal cycle changes of the mice in the control group and the experimental group on the fourth day. period, preestrus, estrus and postestrus; continuous monitoring for 3 weeks.

[0064] The results showed that the average estrous cycle of mice in the control group was 5.13±0.24 days, and the estrous cycle was prolonged to 9.66±0.80 days after col...

Embodiment 2

[0067] Example 2 Changes in the expression level of CX43 in ovarian tissue after cold exposure

[0068] Considering that the gap junction protein CX43 has a key role in regulating the development and maturation of follicles, the present invention detects the changes in the expression level of CX43 in ovarian tissue after exposure to cold.

[0069] The results showed that compared with the control mice, the expression level of CX43 in the ovarian tissue of the cold-exposed mice was significantly up-regulated ( image 3 A-3B, P image 3 C, P<0.05). The above results indicate that cold exposure can significantly affect the expression level of the gap protein CX43 in ovarian tissue.

[0070] Since the gap junction protein CX43 is expressed in high abundance in granulosa cells, the present invention separates and cultured primary granulosa cells, and cultured them in normal temperature (37°C) and low temperature (35°C) conditions for 12 hours, and found that cold exposure The expr...

Embodiment 3

[0076] Example 3 Effects of cold exposure on the expression and activation level of PERK in ovarian tissue

[0077] The present invention detects the expression and activation level of PERK in mouse model ovarian tissue.

[0078] It was found that cold exposure can induce significant activation of PERK in ovarian tissue ( Figure 4 D-4E, P<0.05), suggesting that PERK may affect the inducible expression of CX43 by regulating the downstream molecule NRF2.

[0079] Subsequently, in the present invention, the primary granulosa cells were cultured at normal temperature (37°C) and low temperature (35°C) for 12 hours to detect the activation of PERK / NRF2 pathway.

[0080] The results showed that the primary granulosa cells were significantly induced and activated by PERK and NRF2 after exposure to 35°C ( Figure 5 A).

[0081] The above results indicate that low temperature exposure induces significant activation of the PERK / NRF2 pathway in primary granulosa cells, which is consis...

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Abstract

The invention discloses application of PERK in prevention or treatment of female reproductive dysfunction or ovarian lesion. It is found that inhibition of PERK expression or activation level can significantly antagonize the reproductive cycle disorder effect and follicular dysplasia effect induced by cold exposure. The invention proves that PERK can be used as a new target for preventing or treating female reproductive dysfunction or ovarian lesion for the first time.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to the application of PERK in preventing or treating female reproductive dysfunction or ovarian disease. Background technique [0002] Changes in ambient temperature can affect the physiological functions of normal organisms. Cold, as an important environmental factor, can cause damage to multiple systems of the organism and pose a serious threat to people's health. Exposure to cold environment is widely recognized as a global challenge to human health, and it is of great significance to explore measures to reduce the impact of cold stress on the body. Given that the human body is not as adaptable to cold as it is to high temperature (V Klenerová, Jana Juroviová, Kaminsky O, et al. Combined restraint and cold stress in rats: effects on memoryprocessing in passive avoidance task and on plasma levels of ACTH and corticosterone[J ].Behavioural Brain Research,2003,142(1-2):143-149.), cold ...

Claims

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Application Information

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IPC IPC(8): A61K45/00A61K31/713A61P15/00C12Q1/6883G01N33/68
CPCA61K45/00A61K31/713A61P15/00C12Q1/6883G01N33/6893C12Q2600/158G01N2333/47G01N2800/367
Inventor 宋伦刘琨丁萌楠卢亚蓉黄欣邢陈张艺凡王伟张冲冲
Owner ACADEMY OF MILITARY MEDICAL SCI
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