High-sensitivity Raman enhanced substrate for rapid detection and preparation method thereof

Abstract

The invention provides a high-sensitivity Raman enhanced substrate for rapid detection. The high-sensitivity Raman enhanced substrate comprises a metal substrate, and a micropore array, a super-hydrophobic area and a super-hydrophilic area are prepared on the surface of the metal substrate; the micropore array is composed of a plurality of micropores, the peripheral annular area of the micropores is a super-hydrophilic area, and the other areas are super-hydrophobic areas. According to the method, the surface of the metal material is ablated by high-power ultrafast pulse laser to form a periodic micro-nano structure, the super-hydrophobic, super-hydrophilic and microporous mixed metal surface is prepared in cooperation with a surface modifier of a low-free-energy substance, the process is simple, implementation is easy, and good flexibility and designability are achieved. The Raman substrate provided by the invention has unique advantages in the aspects of attraction anchoring, enrichment effect and low sample dosage, and can directly and quickly locate the deposition position of a target to-be-detected molecule during detection. The invention also provides a preparation method of the high-sensitivity Raman enhanced substrate for rapid detection.

Description

technical field [0001] The invention relates to a Raman-enhanced substrate, in particular to a high-sensitivity Raman-enhanced substrate for rapid detection and a preparation method thereof. Background technique [0002] Ultratrace molecular detection has shown its potential in environmental monitoring, precancer diagnosis, food safety, etc., and will be more promising in early disease diagnosis. Among various analytical techniques, Surface Enhanced Raman Scattering (SERS) is becoming one of the cutting-edge techniques for tracking molecules at very low concentrations due to its fast, high sensitivity and non-destructive advantages. Existing surface Raman-enhanced substrates are roughly divided into: hydrophilic substrates, superhydrophobic substrates, and mixed superhydrophobic / superhydrophilic substrates. However, the existing superhydrophobic surface-enhanced Raman substrates have low adhesion, and the sliding of droplets leads to random molecular deposition positions, m...

AI Technical Summary

Problems solved by technology

However, the existing super-hydrophobic surface-enhanced Raman substrate has low adhesion, and the sliding of the droplet leads to the randomness of the molecular deposition position, making it difficult to directly and quickly locate the target molecular deposition position.

In addition, due to the single-period structure of the low-adhesion superhydrophobic surface, it is difficult to quickly capture trace amounts of colorless deposits even with microscopic Raman equipment.

In addition, on hybrid superhydrophobic / superhydrophilic substrates, the central region of the superhydrophilic pattern can still detect the residue of target molecules.

At the same time, the size of the superhydrophilic pattern cannot be reduced indefinitely, resulting in a decrease in detection performance.

In addition, most traditional methods based on chemical synthesis or complex lithography routes, such as focused ion beam and electron beam lithography, suffer from non-uniformity or low throughput

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