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Application of SOD in insect virus insect killing

A technology of superoxide and dismutase, applied in application, biocide, animal repellent, etc., can solve the problems of short virus residual time and poor field effect.

Inactive Publication Date: 2005-04-20
WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0015] However, no matter whether wild type or recombinant insect virus, its field effect is not as significant as that of laboratory bioassay, the main reason is that the residual time of the virus in the field environment is too short

Method used

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  • Application of SOD in insect virus insect killing
  • Application of SOD in insect virus insect killing

Examples

Experimental program
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Effect test

Embodiment 1

[0028] For three consecutive days, 0.025 units / μL and 0.25 units / μL of superoxide dismutase SOD and 1×10 5 PIB / mL mixed with Helicoverpa armigera single-nucleocapsid polyhedrovirus (HaSNPV), drop 10 μL on fresh cotton leaves, or use 1×10 5 PIB / mL HaSNPV was added dropwise; 72 second-instar cotton bollworm larvae (meeting the statistical standard) were put on the leaves, allowed to feed, and cultured at 28°C. On the fourth day, they were transferred to fresh artificial feed (to ensure the nutrition required for pupation), cultured until pupation, and the mortality rate was recorded every day. A control group was set up; the data were analyzed by variance with statistical methods. The experiment was repeated three times. The results showed that SOD of 0.025 unit / μL and 0.25 unit / μL increased the infectivity of HaSNPV to cotton bollworm by 24.5% and 43.1% respectively (commercialized SOD came from Roche Company, extracted from calf serum, and was Cu / Zn-SOD). Therefore, Cu / Zn-S...

Embodiment 2

[0030] Helicoverpa armigera single nucleocapsid polyhedrovirus (HaSNPV) is an insect virus specific to cotton bollworm. Its genome contains the sod gene, with a size of 480bp, encoding Cu / Zn-SOD. Using polymerase chain reaction (Polymerase Chain Reaction, PCR) to amplify the nucleotide sequence of about 1k at both ends of the sod gene as homology arms, and the marker gene LacZ The sod gene and the HaSNPV endogenous promoter p10 were inserted into the co-cloned pBlueScript ii plus vector, and then the vector was co-transfected with the HaSNPV wild-type eukaryotic cell HzAM1, and after plaque selection and purification, a recombinant virus overexpressing SOD enzyme was obtained . The survival time of the virus in the field will be longer than that of the wild type virus, and the field effect of the insect virus insecticide made with the virus will be stronger than that of the wild type virus insecticide. Cotton bollworm virus HaSNPV carrying superoxide dismutase Cu / Zn-SOD gene ...

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Abstract

The invention discloses an application of superoxide dismutase in killing insect viruses. The superoxide dismutase can enhance the infectivity of cotton bollworm virus on cotton leaves. The substance can be used to enhance the insecticidal effect of the insect virus insecticide in the field. Experiments have shown that SOD can increase the infection activity of cotton bollworm virus to cotton bollworm larvae raised on cotton leaves. SOD can be directly used as a synergist added to insect virus insecticides, and molecular biology methods can also be used to construct recombinant viruses that overexpress SOD enzymes. The effect of this recombinant virus as an insecticide is better than that of general insect virus insecticides. .

Description

technical field [0001] The present invention relates to the use of an enzyme, that is, superoxide dismutase (Superoxide Dismutase, SOD), in particular to the use in killing insect viruses. Background technique [0002] 1. Properties of superoxide dismutase [0003] Superoxide Dismutase (Superoxide Dismutase) is referred to as SOD. In 1969, McCord and Fridovich found that blood copper protein, liver copper protein and brain copper protein all have O 2 - Dismutation activity, and this enzyme is named superoxide dismutase. According to the combination of metal ions, it is divided into three categories, namely Cu / Zn-SOD, Mn-SOD and Fe-SOD. [0004] SOD type molecular knot structure molecular weight metal content color Absorption peak(nm) molecular conformation Inhibitor Cu / Zn-SOD two or Tetramer 32000-65000 1Cu:1Zn blue-green ...

Claims

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Application Information

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IPC IPC(8): A01N63/00
Inventor 孙修炼周明哲胡志红
Owner WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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