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Prepn process and application of amylolytic bacillus CH-2 strain

A technology of amylolytic spores and CH-2, which is applied in the field of microorganisms, can solve the problems of polluting the environment, lack of cloning resistance genes, and no ideal effect, etc., and achieves the effects of simple preparation method, inhibition of sclerotia formation, and convenient operation

Inactive Publication Date: 2005-05-18
WUHAN VITAL PHARMA
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Problems solved by technology

However, the use of chemical control not only pollutes the environment, but also the long-term use of fungicides can lead to the emergence of drug-resistant strains. At present, drug-resistant virulent strains have been isolated in many rape planting areas
Since no effective resistance gene against sclerotinia has been cloned at present, the prevention and control by genetic engineering has no ideal effect

Method used

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  • Prepn process and application of amylolytic bacillus CH-2 strain
  • Prepn process and application of amylolytic bacillus CH-2 strain
  • Prepn process and application of amylolytic bacillus CH-2 strain

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Embodiment Construction

[0016] The invention provides a Bacillus amyloliquefaciens CH-2 strain CCTCC M 203055. Also provided is an application of Bacillus amyloliquefaciens CH-2 strain CCTCC M 203055 in the preparation of biological products for preventing and controlling sclerotinia of economic crops caused by Sclerotinia sclerotiorum, and the economic crops are rapeseed, soybean, or sunflower.

[0017] The present invention will be further described below in conjunction with embodiment.

[0018] In the research on the isolation and screening of rapeseed biocontrol bacteria, several strains (for example, 17 strains) of Bacillus strains were isolated from soil samples from different sources, and the effects of these strains on inhibiting Sclerotinia sclerotiorum were tested, of which 4 strains were effective against rapeseed Sclerotinia showed varying degrees of inhibition. These strains include 2 strains of Bacillus subtilis (F10, L5), 1 strain of Bacillus licheniformis (B. licheniformis AJH-1), an...

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Abstract

The invention relates to a preparation method and application of Bacillus amyloliquetaciens CCTCC M203055 strain CH-2. The bacterial strain is used as a biological product for preventing and controlling sclerotinia or sclerotinia in rapeseed, soybean, sunflower and other economic crops. The preparation method of the bacterial strain comprises the steps of activation and cultivation of pathogenic bacteria, isolation, cultivation and screening of bacillus. The Bacillus amyloliquefaciens CH-2 strain obtained by the invention has a strong ability to inhibit the growth of Sclerotinia sclerotiorum and the ability to inhibit the formation of sclerotia, which is beneficial to the large-scale popularization and planting of double-low rapeseed, and also has the advantages of simple preparation method and convenient operation etc., and is suitable for industrialized production, and has great potential value in development and utilization.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to the preparation method and application of the bacillus amyloliquefaciens CH-2 strain. The Bacillus amyloliquetaciens CH-2 (Bacillus amyloliquetaciens CH-2) used in this patent has been preserved by the China Center for Type Culture Collection, the preservation date is June 16, 2003, and the preservation number is CCTCC NO: M 203055. Background technique [0002] Sclerotinia sclerotiorum (Lib.) de Bary] is an important plant pathogenic fungus, which can parasitize more than 450 kinds of plants, including rapeseed, soybean, sunflower and other important oil crops. The sclerotinia caused by it is a kind of A devastating disease with worldwide distribution. S. sclerotiorum survives winter and summer in the soil in the form of sclerotia. When the temperature and humidity conditions are suitable, the sclerotia germinates to produce ascus discs, and the distributed ascospores infect plant...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/00C12N1/20
Inventor 陈士云杨宝玉宋冬林王瑶高梅影戴顺英
Owner WUHAN VITAL PHARMA
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