Fine rod bundle spore SX-1 separated and cultured from cordyceps and its culture process and use

A technology of isolation culture and culture method, applied to the field of new strains isolated and cultured from natural Cordyceps sinensis and its artificial culture, can solve the problems of bat moth larvae reproduction difficulties, low yield of natural Cordyceps sinensis, etc.

Inactive Publication Date: 2005-07-27
任连生
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the output of natural Cordyceps sinensis is extremely low, and it is far from being able to meet people's actual needs.
At present, there is no precedent for large-scale artificial cultivation of C

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0079] Example 1:

[0080] This strain (A. tenuis SX-1) was cultured on PDA medium at 25°C for 10 days. The colonies are round, flat, confined, with rings and wrinkles, initially white, then grayish white, 1.5~2.2cm in diameter. , The backside is gray-brown to purple-brown; the hypha is colorless, branched, with a septum, 1~2.5μm wide; spore bunches are white, branched, 0.5~3.5cm high, 0.2-1.5mm diameter: curved neck flask of spore-forming cells The shape is cylindrical, colorless, curved, 4.8~8.5×1~2.2μm; conidia are elliptical to ovoid, monospore, colorless, smooth, 2.5~4.3×2~3μm.

Example Embodiment

[0081] Example 2:

[0082] Mother seed separation culture

[0083] Prepare PSA medium:

[0084] 200g potato (peeled), 18g agar, 20g sucrose, 1000ml distilled water, pH 6-6.5;

[0085] Take potatoes, wash, peel and cut into pieces, add 1200ml of water, simmer for 20 minutes, filter the juice, make up the amount of water to 1000ml, add agar, sucrose, and after fully dissolving, adjust the pH to 6-6.5 and steam sterilize at 121°C For 30 minutes, take it out and pour it into a sterile plate or test tube while it is hot. After cooling and solidification, place it at 25-28°C and incubate it for 1-2 days. Check that it is free of contamination before use.

[0086] Select Cordyceps sinensis in the Aba area of ​​Sichuan Province as the source, rub the surface of the Cordyceps with 75% alcohol 3 times, 0.8min each time, rinse 4 times with sterile water, and place it in a sterile plate with a lid; absorb with sterile filter paper Remove the surface water, use sterile tweezers to tear apart f...

Example Embodiment

[0087] Example 3:

[0088] Prepare a dry article of Corynespora tenuis SX-1.

[0089] The mother seed is placed in a culture medium to prepare first-grade seeds. The formula of the culture medium is 36 grams of peptone, 72 grams of yeast extract, 144 grams of sucrose, 3 grams of magnesium sulfate, 6 grams of potassium dihydrogen phosphate, 6000 ml of distilled water, and pH adjustment. The value is 6.5. After the medium is prepared, it is steam sterilized at 130°C for 20 minutes, and inoculated when it is cooled to 26±0.5°C.

[0090] Inoculation: Under aseptic conditions, put the above-mentioned culture medium into 6 flasks each with a capacity of 3000 ml, and insert 50 ml of first-class seed into each bottle. Place the Erlenmeyer flask in a rotary shaker at a temperature of 26±0.5°C and a rotation speed of 180 rpm for 48 hours.

[0091]Harvest: The appearance of the culture medium is thick, the pH of the test medium is 6, and there is no bacteria in the microscopy. The supernatan...

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PUM

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Abstract

The present invention is new fungus strain separated from natural Cordyceps and its artificial culture process and use. The fungus strain has the preservation number of CGMCC No.0706 and has the morphologic features of white to grey circular colony of 1.5-2.2 cm diameter; colorless branched mycelial filament with diaphragm of 1-2.5 micron width; white and branched sporophore of 0.5-3.5 cm height and 0.2-1.5 mm diameter. The product of the present invention after being dried has the function of inhibiting tumor and may be used in preparing antineoplastic medicine.

Description

technical field [0001] The invention relates to Cordyceps fungus and its artificial cultivation method and application, in particular to a new bacterial strain isolated and cultivated from natural Cordyceps sinensis and its artificial cultivation method and application. Background technique [0002] Cordyceps sinensis (Cordyceps for short) is a composite of the subseat of the Ergotaceae plant Cordyceps sincesis (Berk.) Sace. and the larvae of its host Hepialus armoricanus Oberthur. It mainly contains amino acids , polysaccharides, cordycepin, cordycepic acid, ergosterol, adenosine, superoxide dismutase and trace elements. Among them, the level of adenosine content has been used as the main basis for drug quality control (see "Pharmacopoeia of the People's Republic of China (2000 Edition)" Part 1 P499: Jinshuibao capsule, quality control standard for fermented Cordyceps powder). Modern pharmacological studies have shown that Cordyceps has sedative, anticonvulsant, cooling, e...

Claims

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Application Information

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IPC IPC(8): A61P35/00C12N1/14
Inventor 刘玉成苏琳邹元生王岳聂勇
Owner 任连生
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