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Fine rod bundle spore SX-1 separated and cultured from cordyceps and its culture process and use

A technology of isolation culture and culture method, applied to the field of new strains isolated and cultured from natural Cordyceps sinensis and its artificial culture, can solve the problems of bat moth larvae reproduction difficulties, low yield of natural Cordyceps sinensis, etc.

Inactive Publication Date: 2005-07-27
任连生
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the output of natural Cordyceps sinensis is extremely low, and it is far from being able to meet people's actual needs.
At present, there is no precedent for large-scale artificial cultivation of Cordyceps sinensis. The main reason is that the bat moth larvae are difficult to reproduce, and there are still many insurmountable problems in the inoculation technology and ecological simulation technology.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] This strain (I. clavulatus SX-1) was cultured on PDA medium at 25°C for 10 days. The colonies were round, flat, limited, with rings and wrinkles, initially white, then off-white, with a diameter of 1.5-2.2 cm. , gray-brown to purple-brown back; mycelia are colorless, branched, septate, 1-2.5 μm wide; spore bundles are white, branched, 0.5-3.5 cm high, 0.2-1.5 mm in diameter: spore-forming cells Cylindrical, colorless, curved, 4.8~8.5×1~2.2μm; conidia oval to oval, monospore, colorless, smooth, 2.5~4.3×2~3μm.

Embodiment 2

[0082] Separation of mother species

[0083] Prepare PSA medium:

[0084] Potato (peeled) 200g, agar 18g, sucrose 20g, distilled water 1000ml, pH value 6-6.5;

[0085] Take potatoes, wash, peel and cut into pieces, add 1200ml of water, boil for 20 minutes, filter to get the juice, make up the water to 1000ml, add agar, sucrose, after fully dissolved, adjust the pH value to 6-6.5, steam sterilization at 121°C After 30 minutes, take it out and pour it into a sterile plate or test tube while it is hot. After cooling and solidifying, place it at 25-28°C and culture it for 1-2 days in a blank. After checking for no pollution, it can be used.

[0086] Cordyceps sinensis in Aba area of ​​Sichuan was selected as the provenance, and the surface of Cordyceps was rubbed with 75% alcohol three times, each time for 0.8 min, washed with sterile water for four times, placed in a sterile plate with a cover; blotted with sterile filter paper Remove the surface moisture, use sterile tweezers ...

Embodiment 3

[0088] Prepare dried items of I. clavula SX-1.

[0089] Put the mother seed in the culture medium to prepare first-class seeds. The formula of the culture medium is 36 grams of peptone, 72 grams of yeast extract, 144 grams of sucrose, 3 grams of magnesium sulfate, 6 grams of potassium dihydrogen phosphate, 6000 ml of distilled water, and adjust the pH Value 6.5. After the medium is prepared, steam sterilize at 130°C for 20 minutes, and inoculate when cooled to 26±0.5°C.

[0090] Inoculation: Under aseptic conditions, put the above-mentioned medium into 6 Erlenmeyer flasks with a capacity of 3000 ml, and insert 50 ml of the first-grade species into each bottle. Place the Erlenmeyer flask in a rotary shaker for 48 hours at a temperature of 26±0.5°C and a rotation speed of 180 rpm.

[0091]Harvest: The appearance of the culture solution is thick, the pH value of the culture medium is 6, and there is no miscellaneous bacteria in the microscopic examination. Centrifuge at 2000 rp...

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PUM

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Abstract

The present invention is new fungus strain separated from natural Cordyceps and its artificial culture process and use. The fungus strain has the preservation number of CGMCC No.0706 and has the morphologic features of white to grey circular colony of 1.5-2.2 cm diameter; colorless branched mycelial filament with diaphragm of 1-2.5 micron width; white and branched sporophore of 0.5-3.5 cm height and 0.2-1.5 mm diameter. The product of the present invention after being dried has the function of inhibiting tumor and may be used in preparing antineoplastic medicine.

Description

technical field [0001] The invention relates to Cordyceps fungus and its artificial cultivation method and application, in particular to a new bacterial strain isolated and cultivated from natural Cordyceps sinensis and its artificial cultivation method and application. Background technique [0002] Cordyceps sinensis (Cordyceps for short) is a composite of the subseat of the Ergotaceae plant Cordyceps sincesis (Berk.) Sace. and the larvae of its host Hepialus armoricanus Oberthur. It mainly contains amino acids , polysaccharides, cordycepin, cordycepic acid, ergosterol, adenosine, superoxide dismutase and trace elements. Among them, the level of adenosine content has been used as the main basis for drug quality control (see "Pharmacopoeia of the People's Republic of China (2000 Edition)" Part 1 P499: Jinshuibao capsule, quality control standard for fermented Cordyceps powder). Modern pharmacological studies have shown that Cordyceps has sedative, anticonvulsant, cooling, e...

Claims

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Application Information

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IPC IPC(8): A61P35/00C12N1/14
Inventor 刘玉成苏琳邹元生王岳聂勇
Owner 任连生
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