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A fragrance-producing Pichia kudriazvii strain that degrades ammonia nitrogen and its application

A technology for degrading ammonia nitrogen and yeast, which is applied in the direction of microorganisms, fungi, microorganisms, etc., can solve the problems of ammonia nitrogen degradation that have not been reported, and achieve the effect of being suitable for large-scale industrial production, stable strains, and expanding environmental conditions

Active Publication Date: 2021-06-25
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Pichia pastoris strains that degrade ammonia nitrogen have not been reported

Method used

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  • A fragrance-producing Pichia kudriazvii strain that degrades ammonia nitrogen and its application
  • A fragrance-producing Pichia kudriazvii strain that degrades ammonia nitrogen and its application
  • A fragrance-producing Pichia kudriazvii strain that degrades ammonia nitrogen and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Isolation and identification of Pichia pastoris HJ2

[0045] (1) Sampling was carried out from mangrove soil sediments in Beihai City, Guangxi Province. After the samples were dissolved in sterile water, they were shaken and cultured on a shaker at 37°C for 24 hours, and then diluted to 10 with sterile distilled water. -1 、10 -2 、10 -3 、10 -4 、10 -5 , take 10 respectively -2 ~10 -5 100 μL of the diluted bacterial solution was spread on YPD solid medium respectively, cultured at 37°C for 2 to 3 days, and single colonies with milky white, slightly convex, moist, irregular edges, round or oval, and rough surface were picked and plated Streak 2-3 times to obtain a single colony.

[0046] (2) Activation of Pichia pastoris HJ2

[0047] The strains taken out from -80°C were inoculated and streaked in YPD solid medium, and cultured at 37°C for 48 hours;

[0048] (3) The whole genome of Pichia pastoris HJ2 was sequenced.

[0049] Pichia pastoris HJ2 was sent t...

Embodiment 2

[0078] Example 2 Headspace Gas Chromatography Detects Volatile Fragrance Substances

[0079] (1) Determination of fermentation flavor

[0080] Take YPD medium and glucose medium (aroma production fermentation medium), take 2% of the volume percentage as the inoculum size, 37°C, 200 rpm, culture for 12 hours, 1 day, 3 days, 5 days, 7 days, Smell the flavor of the liquid fermentation liquid, the liquid fermentation liquid has special aromas such as sweet aroma and fruit acid.

[0081] (2) Detection of special aroma metabolites

[0082] The samples fermented by Pichia kudriazweiwei HJ2 for 12 hours, 1 day, 3 days, 5 days and 7 days were treated with YPD medium and glucose medium respectively, and analyzed by headspace gas chromatography. GC conditions: DB-5MS elastic quartz capillary chromatographic column SH-Rxi-5Sil MS chromatographic column (30m×0.25mm×0.25μm) for separation; the temperature programming condition is the initial temperature of 40°C, and the temperature is rai...

Embodiment 5

[0091] Embodiment 5 salicylic acid colorimetric method measures ammonia nitrogen degradation efficiency

[0092] (1) Preparation of ammonia nitrogen medium

[0093] Medium: glucose 20g, ammonium sulfate 0.25g, sodium chloride 1.0g, dipotassium hydrogen phosphate 0.5g, heptahydrate and magnesium sulfate 0.25g, trace element solution 1mL, distilled water 1L. Take 100mL of the prepared solution and divide it into 250mL Erlenmeyer flasks, sterilize at 115°C for 15 minutes, and the ammonia nitrogen content in the uninoculated ammonia nitrogen medium is 50mg / L.

[0094] Trace element solution: 10.0g EDTA, 1.2g zinc sulfate, 1.5g calcium chloride, 1.0g manganese chloride tetrahydrate, 2.0g ferrous sulfate, 1.0g ammonium molybdate, 1.0g pentahydrate and copper sulfate, cobalt chloride 1.0g, distilled water 1L pH 7.2-7.4.

[0095] The initial ammonia nitrogen concentration of the medium is 50 mg / L, and the ammonium sulfate content is adjusted proportionally so that the initial ammonia ...

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Abstract

The invention belongs to the technical field of industrial microbes, and relates to an aroma-producing Pichia kudriazwi for degrading ammonia nitrogen and its application. An aroma-producing Pichia kudriavzevii that degrades ammonia nitrogen, which is classified as Pichia kudriavzevii HJ2, and was preserved in China Microorganism Culture Collection on June 1, 2020 Committee General Microbiology Center, deposit number is CGMCC No.19897. The Pichia kudriazvii provided by the present invention can use five-carbon sugar and six-carbon sugar organic matter alone to produce rich aromatic compounds, is resistant to salt and heavy metals, and can adapt to a wide range of pH, expanding the treatment of ammoniacal nitrogen Environmental conditions of wastewater. Using the strain of the invention to treat the water body containing high concentration of ammonia nitrogen, the production process is environmentally friendly, the operation is simple, and there is no secondary pollution, which is very suitable for large-scale industrial production.

Description

technical field [0001] The invention belongs to the technical field of industrial microbes, and relates to an aroma-producing Pichia kudriazwi for degrading ammonia nitrogen and its application. Background technique [0002] Nitrogen in ammonia nitrogen wastewater is represented by ammonium ions (NH 4 + ) and free ammonia (NH 3 ) exists in water in the form of domestic sewage and landfill leachate, or industrial production such as steel, oil refining, petrochemical, glass manufacturing, fertilizer, leather tanning, feed production, etc. will produce a large amount of ammonia nitrogen wastewater, which has a wide range of sources. Wastewater The high oxygen consumption of ammonia nitrogen in the medium will lead to eutrophication of the water body, forming "algae bloom" and "red tide" to destroy the water body environment, and have a toxic effect on aquatic organisms. Humans will endanger their lives if they eat polluted aquatic products by mistake. [0003] In recent year...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C02F3/34C12R1/84C02F101/16
CPCC02F3/347C02F2101/16C12N1/165C12R2001/84
Inventor 蒋承建于苒何升阎冰莫雪艳惠秦妍蔡杏华卜孺孙会杰陈思
Owner GUANGXI UNIV
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