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New qualitative and/or quantitative determination method

A quantitative detection method and technology of detection content, applied in the new qualitative and/or quantitative detection field, can solve the problems of density recognition distortion, position recognition distortion, position error, etc., to improve specificity and sensitivity, improve certainty, increase optional effects

Inactive Publication Date: 2005-08-03
CHENGDU KUACHANG SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the simple repeated use of the probe circles does not produce enough information for pattern recognition, and the random deformation of the probe circles in the actual printing process, the existing chips only have positional and density properties, but no graphic properties.
Any position-related interference, such as position errors, non-specific marks, etc., can cause distortion of position recognition and thus density recognition
In other words, even if the existing biochip method uses an expensive positioning system (high-precision printing equipment, high-precision scanner and position processing software, etc.), the detection results still have high uncertainty
[0005] In short, for the existing detection methods, due to the use of position recognition and / or density recognition, they have been more and more widely used, but because they only use density recognition and / or position recognition, the accuracy of the detection results Uncertainty still needs to be further reduced

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Production example of a novel rapid diagnostic reagent strip

[0035] In this example, the solid phase carrier of the reagent strip is a plastic substrate with a width of 8 mm. One end of the substrate is the sample adding area, the other end is the water absorption area, and the middle section is the water absorption fiber membrane observation area. The probes are HAV antigen, HCV fusion antigen, HDV antigen, HEV antigen and HGV antigen, these antigens are fixed on the observation area (6×6mm), each antigen is fixed as an image, and the antigen coating pattern They are: HAV antigen probe in the form of an upward parabola, HCV antigen probe in the form of a downward parabola, HDV fusion antigen probe in the form of a horizontal line, HEV antigen probe in the form of a leftward parabola, HGV probe in the form of an opening The rightward parabolic pattern and protein A as a reference were distributed in a vertical straight pattern (Figure 1). The printing of t...

Embodiment 2

[0036] Example 2: A production example of a fluorescently labeled chip kit

[0037] In the kit in this example, the solid phase carrier of the probe plate is an activated microscope slide (25×75mm). After this glass slide is bonded to a molded plastic plate, the probe card is fabricated to contain 7 parallel selective reactors (Fig. 2), each with a size of 18×4×1mm (length×width). × high). The probes are: hepatitis B e antigen, hepatitis C NS3, NS4, NS5, CORE fusion antigen, genetically engineered fusion antigen containing gp120, gp41, p24 of HIV1 and gp36 fragment of HIV2, syphilis antigen, HTLV antigen, and as a control The protein A of the substance. Each reactor is printed with 6 probe characters, each character is 2 x 2 mm in size. The characters A, B, C, D, E and F are stamped with the Hepatitis B e Antigen, Hepatitis C Fusion Antigen, HIV1+2 Fusion Antigen, Syphilis Antigen, HTLV Antigen and Protein A, respectively, with a stamp. The activity in the reactor where th...

Embodiment 3

[0038] Example 3: Production example of a gold-silver dyeing chip kit

[0039] In the kit in this example, the solid phase carrier of the probe plate is an activated microscope slide (25×75mm). The probes are: hepatitis C NS3, NS4, core antigen, and protein A as a control; the graphic symbols of the probes are: equilateral triangle, circle, pentagon and rectangle, as shown in the figure below. The external dimension of each symbol is 0.5×0.5mm, and it is printed with a seal respectively. Activity on slides without symbols was inactivated with blocking solution. The labeling systems in the kit are colloidal gold-labeled goat anti-human IgG and silver nitrate amplified for the labeled gold.

[0040] Colloidal gold-silver staining chip kit test results

[0041] Sample Test Results

[0042] NS3 NS4 CORE

[0043] Negative control 79(-) 85(-) 115(-)

[0044] Positive control 1271(++) 913(+) 1721(+++)

[0045] Test sample 1 1354(++) 79(-) 135(-)

[0046] Tes...

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PUM

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Abstract

The invention discloses a new qualitative and / or quantative detection method for the target compound in the specimen possessing the biological activity. The technique of the graphic recognition is utilized in the method to be as the detection item or the one of the detection items. Comparing with the prior art, which recognizes the densities and the positions, the invented method raises the determinancy of the detected results further so as to increase the specificity and the sensibility of the detection.

Description

Technical field: [0001] The present invention relates to a method for detecting a sample, especially a method for qualitatively and / or quantitatively detecting a target compound in a sample containing biological activity. Detection method. Background technique: [0002] At present, methods for qualitative and / or quantitative detection of target substances in samples, especially biologically active samples, are based on two identification principles of density identification and / or position identification. Density identification means that the detection probe is fixed in the reactor on the probe plate in a quantifiable manner, and then during the detection process, the density of the captured target or the density of the label that interacts with the target is determined. , an informative analysis performed under comparable conditions by comparison with known densities of captured known targets or known markers. In actual detection, the density of the target object or the d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68G01N33/50G01N33/53G01N33/543G01N33/567G01N33/68
Inventor 邹方霖陈春生胡劲梅王建霞
Owner CHENGDU KUACHANG SCI & TECH CO LTD