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New qualitative and/or quantitative determination method

A quantitative detection method and technology of detection content, applied in the new qualitative and/or quantitative detection field, can solve the problems of density recognition distortion, position recognition distortion, position error, etc., to improve specificity and sensitivity, increase selectivity, The effect of improving certainty

Inactive Publication Date: 2003-04-30
CHENGDU KUACHANG SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the simple repeated use of the probe circles does not produce enough information for pattern recognition, and the random deformation of the probe circles in the actual printing process, the existing chips only have positional and density properties, but no graphic properties.
Any position-related interference, such as position errors, non-specific marks, etc., can cause distortion of position recognition and thus density recognition
In other words, even if the existing biochip method uses an expensive positioning system (high-precision printing equipment, high-precision scanner and position processing software, etc.), the detection results still have high uncertainty
[0005] In short, for the existing detection methods, due to the use of position recognition and / or density recognition, they have been more and more widely used, but because they only use density recognition and / or position recognition, the accuracy of the detection results Uncertainty still needs to be further reduced

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: A production example of a novel rapid diagnostic reagent strip

[0035] In this example, the solid phase carrier of the reagent strip is a plastic substrate with a width of 8mm. One end of the substrate is the sample loading area, the other end is the water absorption area, and the middle section is the water absorption fiber membrane observation area. The probes are HAV antigen, HCV fusion antigen, HDV antigen, HEV antigen and HGV antigen. These antigens are fixed on the observation area (6×6mm), and each antigen is fixed as an image, and the antigen is coated with a pattern They are: HAV antigen probe in the form of an open upward parabola, HCV antigen probe in the form of an open downward parabola, HDV fusion antigen probe in the form of a horizontal straight line, HEV antigen probe in the form of an open left parabola, HGV probe in the form of an open Parabolic pattern to the right and protein A as a reference are distributed in a vertical straight pat...

Embodiment 2

[0036] Embodiment 2: Production example of a fluorescent labeling chip kit

[0037] In the kit in this example, the solid phase carrier of the probe card is an activated microscope slide (25×75 mm). After this glass slide is bonded with a plastic plate that has been formed, the probe card is made into 7 parallel selective reactors (Fig. x high). The probes are respectively: hepatitis B e antigen, hepatitis C antigen, genetically engineered antigen containing HIV1, syphilis antigen, HTLV antigen, and protein A as a control. There are 6 probe characters printed in each reactor, and the size of each character is 2X2mm. Characters A, B, C, D, E and F are printed with seals using hepatitis B e antigen, hepatitis C fusion antigen, HIV1+2 fusion antigen, syphilis antigen, HTLV antigen and protein A respectively. The activity of the unmarked place in the reactor was inactivated with blocking solution. The labeling system in the kit is rhodamine-labeled goat anti-human IgG.

Embodiment 3

[0038] Embodiment 3: the production example of a kind of gold and silver staining chip kit

[0039] In the kit in this example, the solid phase carrier of the probe card is an activated microscope slide (25×75mm). The probes are: hepatitis C NS3, NS4, core antigen, and protein A as a control; the graphic symbols of the probes are: equilateral triangle, circle, pentagon and rectangle. The outer dimension of each symbol is 0.5×0.5mm, and it is printed with a seal respectively. The activity on the slide without symbols was inactivated with blocking solution. The labeling system in the kit is goat anti-human IgG labeled with colloidal gold and silver nitrate amplified for labeled gold.

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PUM

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Abstract

The invention discloses a new qualitative and / or quantative detection method for the target compound in the specimen possessing the biological activity. The technique of the graphic recognition is utilized in the method to be as the detection item or the one of the detection items. Comparing with the prior art, which recognizes the densities and the positions, the invented method raises the determinancy of the detected results further so as to increase the specificity and the sensibility of the detection.

Description

Technical field: [0001] The invention relates to a detection method for samples, especially a qualitative and / or quantitative detection method for target compounds in samples containing biological activity, which is a basic detection content or one of the basic detection content detection method. Background technique: [0002] At present, methods for qualitative and / or quantitative detection of target substances in samples, especially samples containing biological activity, are based on two recognition principles: density recognition and / or position recognition. Density recognition means that the detection probe is immobilized in the reactor on the probe plate in a quantifiable manner, and then during the detection process, the density of the captured target or the density of the marker that interacts with the target , compared with known densities of captured known targets or known markers under comparable conditions for information analysis. In actual detection, the dens...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/50G01N33/53G01N33/543G01N33/567G01N33/68
Inventor 邹方霖陈春生胡劲梅王建霞
Owner CHENGDU KUACHANG SCI & TECH CO LTD