Carbofuran pesticide residual degraded bacteria and its produced bacterial agent
A pesticide residue degradation and carbofuran technology, which is applied in the field of high biotechnology, can solve the problems of low production and use costs, achieve low production costs, appropriate survival time, and protect the ecological environment
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[0024] 1 Isolation and identification of strains
[0025] Take the activated sludge particles domesticated in the laboratory, dissolve them in sterile water, and perform gradient dilution. The diluted solution is coated on 100mg / L carbofuran inorganic salt ion solid medium, and cultured at 30°C for several days. Pick a single colony from it (see figure 1 ), identified as Sphingomonas agrestis. The main biological characteristic is G - , The bacteria are long rod-shaped, slightly curly, no spores, aerobic growth, catalase positive; can oxidize pentose, hexose and disaccharide to produce acid, but do not decompose polyhydric alcohol and inulin. It can grow with carbofuran as the only carbon source and nitrogen source, and completely mineralize it into CO 2 And water and simple inorganic compounds. Under laboratory conditions, the degradation rate of carbofuran can reach more than 95%. The bacterium can be produced with general fermentation equipment in the fermentation ind...
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