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Extraction of amino acid by electric fractionation

An amino acid and extraction technology, applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., can solve the problems of increasing property differences, loss of amino acid activity and characteristics, and achieve mild conditions, simple equipment, and separation selectivity. strong effect

Inactive Publication Date: 2006-03-22
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Physical properties such as: pH, amphoteric properties, solubility, molecular size, polarity, adsorption of adsorbents, etc.; or use chemical properties such as the formation of various salts or reversible derivatives to increase the difference in properties from impurities, thereby The purpose of separation is achieved, but with the irreversible change of the target amino acid and the resulting racemization, the activity and characteristics of the amino acid may be lost

Method used

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  • Extraction of amino acid by electric fractionation
  • Extraction of amino acid by electric fractionation
  • Extraction of amino acid by electric fractionation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A certain amount of polyethylene glycol and dextran were weighed, and prepared into an aqueous solution with a mass percent composition of 5% polyethylene glycol and 5% dextran, respectively. Shake the two-phase aqueous solution and centrifuge to separate the phases to obtain a blank two-phase aqueous system. The pH value of the blank aqueous two-phase system was adjusted to 4.0 with phosphoric acid-disodium hydrogen phosphate buffer solution. The upper phase is called the PEG phase, and the lower phase is called the DEX phase. Phosphoric acid-disodium hydrogen phosphate buffer solution was used as the electrode chamber solution. The two phases of the blank two-phase aqueous system were placed in a graduated cylinder and placed in a water bath, and the temperature of the solution was controlled to room temperature. First start the constant-current pump for conveying the electrode liquid, and then start the constant-current pump for conveying the lower-phase fluid afte...

Embodiment 2

[0031] Prepare the blank two-phase aqueous system described in Example 1, and add β-cyclodextrin to it to saturation. Its pH was adjusted to 4.0 with phosphoric acid-disodium hydrogen phosphate buffer solution. Phosphoric acid-disodium hydrogen phosphate buffer solution was used as the electrode chamber solution. The two phases of the aqueous two-phase system saturated with β-cyclodextrin were respectively placed in a graduated cylinder and placed in a water bath, and the temperature of the solution was controlled to room temperature. First start the constant-current pump for conveying the electrode liquid, and then start the constant-current pump for conveying the lower-phase fluid after the electrode chamber is full, and start the constant-current pump for conveying the upper-phase fluid when the lower-phase fluid reaches half the height of the two-phase chamber . After the two-phase flow is stable, adjust the two-phase flow, control the flow of the upper phase to 0.2ml / mi...

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Abstract

An electric fractionating process for the effective separation of multi-component amino acids, mixture system features the combination of the dual-water-phase electrophoretic extraction and fractionation extraction. After upper and lower phases of dual water phases are separated, both are respectively pumped in two-phase electrophoresis equipment. The solution of amino acids to be separated is injected in it and the DC is applied for extracting separation. Its advantages are simple process and equipment and high selectivity.

Description

technical field [0001] The invention belongs to the separation technology of chemical and pharmaceutical products, in particular to an amino acid electric fractionation extraction method. Background technique [0002] Amino acid is the basic unit of protein, and protein is the main component of living organisms and the basis of life activities. The biological functions of many proteins in organisms are all closely related to the type, quantity, sequence and spatial conformation of the amino acids that make up the protein, so it is of great significance to study the separation of amino acids. [0003] Methods for separating amino acids include ion exchange, electrodialysis, solvent extraction, and electrophoretic separation. In principle, each method generally uses some physical or chemical properties of amino acids to separate them. Physical properties such as: pH, amphoteric properties, solubility, molecular size, polarity, adsorption of adsorbents, etc.; or use chemical ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C227/40
Inventor 骆广生刘蕾亓晅吕阳成
Owner TSINGHUA UNIV