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Process for extracting oleanolic acid-3-O-beta-D pyrane glucuronide from general aralia chinensis saponin and its usage

A technology of glucopyranose and oleanolic acid, which is applied in the directions of steroids, cardiovascular system diseases, organic chemistry, etc., can solve the problem of lack of oleanolic acid-3-0-β-D glucuronide Extraction methods, etc.

Inactive Publication Date: 2006-05-31
吉林省中医中药研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no extraction method for oleanolic acid-3-0-β-D glucopyranoside

Method used

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Examples

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Embodiment

[0012] The present invention provides the following examples:

[0013] The method for extracting oleanolic acid-3-0-β-D glucopyranoside from the total saponins of Aralia bark is to weigh 10 g of total bark glycosides of Aralia bark and dissolve it in 1500ml of water, and then add 0.4mol / L sulfuric acid Mix 1500ml of the aqueous solution, heat to reflux at 100°C for 3 hours, let stand at room temperature for 12 hours, and collect the precipitate by filtration. Add 1000ml of 1% sodium hydroxide aqueous solution to dissolve the precipitate, pass through the macroporous adsorption resin column, wash with deionized water until neutral, then wash with 30% ethanol until colorless, discard the eluent, and then use 60%-80% ethanol elution. The eluate was collected, and ethanol was recovered under reduced pressure to obtain 1100 mg of white powder with a yield of 11%. Melting point (mp) 236-238°C, infrared (IP) (KBr) Cm -1 : 3390 (-OH), 2940, 1682 (-COOH), the molecular weight (M+Na)...

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Abstract

A method and application of extracting oleanolic acid-3-O-β-D glucopyranoside from total saponins of arborescens. Use the produced total saponins as raw material, dissolve the finished total saponins in water or ethanol, then add acid aqueous solution to mix with it evenly, or mix with total saponins ethanol solution, the concentration after mixing is 0.1mol / L -0.8mol / L, the heating temperature is 70-150°C under normal pressure, and the heating time is 1-5 hours. After standing at room temperature for 12 hours, filter the precipitate and dissolve it with 1% sodium hydroxide aqueous solution, and pass it through the macroporous adsorption resin. The column was first eluted with deionized water to neutrality, then washed with 10%-30% ethanol until colorless, discarded the eluent, followed by elution with 60%-80% ethanol, and collected the eluate. The solvent is recovered to obtain the finished product. The oleanolic acid-3-O-β-D glucopyranoside of the present invention is used for the prevention and treatment of heart and cerebrovascular diseases.

Description

Technical field: [0001] The invention belongs to the technical field of purification and application of active ingredients of plant medicines. Background technique: [0002] Aralia etata, Aralia armafa, Aralia spinifolia, Aralia decaisneana, Aralia schmidtii, Aralia contintalis, etc. Araliaceae Araliaceae plant. The leaves, stems, stem bark, root and root bark of these plants contain a variety of aralia saponins. Years of research have shown that aralia saponins are mainly aralia saponins A.C.D.E.F.G.H.I, oleanolic acid-3-0-β -D glucopyranoside is 80 saponins such as deglucose ginseng saponin I Na and compassin. The research of the inventors proves that the total saponins of Aralia have obvious adaptogenic effects, exhibit small-dose excitation and large-dose inhibition to the central nervous system, and have various physiological activities such as anti-myocardial ischemia, anti-arrhythmia, and anti-oxidation. The extraction method of total saponins and its application i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J9/00C07J63/00A61P9/10
Inventor 孙晓波崔东滨徐惠波魏菲温富春王淑琴周继胡丁涛
Owner 吉林省中医中药研究院