Combination gene probe for sulphate reduction pronucleus bioinstrumentation and detecting process thereof
A gene probe and prokaryotic technology, applied in the field of microbial detection, can solve the problems of incomplete detection and time-consuming extinct dilution method, and achieve the effect of low cost and accurate regulation
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Embodiment 1
[0019] Embodiment 1. The SRPs in the effluent water samples of Gudao Oil Production Plant of Shengli Oilfield are detected:
[0020] 1) Prepare a combined gene probe solution, 8 kinds of probes I-VIII of the present invention are made in relative proportions of 15%, 8%, 16%, 11%, 15%, 10%, 6% and 19% Combined gene probe solution;
[0021] 2) Take a water sample from the oil field drainage, and fix the microbial cells including SRPs in the water sample with a concentration of 2% isopolyaldehyde solution;
[0022] 3) Take 3 μl of the above-mentioned fixed microbial cell water sample and drop it into a glass slide, dry the fixed microbial sample on the glass slide at room temperature, and then use 50%, 80% and 100% alcohol in turn to the glass slide Dehydration of microbial samples on the chip;
[0023] 4) Add 3 μl of the combined gene probe solution to the water sample on the glass slide, add a hybridization solution containing 2.0 M NaCl and 10% formamide, and hybridize at a ...
Embodiment 2
[0027] Example 2: Detection of SRPs in the outflow sample of Shengli Oil Production Plant in Shengli Oilfield
[0028] 1) Prepare a combined gene probe solution, and make a combined gene probe solution with relative ratios of 15%, 30%, 20% and 35% of the four probes I, IV, V and VII of the present invention;
[0029] 2) Take a water sample from the oil field drainage, and fix the microbial cells including SRPs in the water sample with a concentration of 8% isopolyaldehyde solution;
[0030] 3) Take 10 μl of the above-mentioned fixed microbial cell water sample and drop it into a glass slide, dry the fixed microbial sample on the glass slide at room temperature, and then use 50%, 80% and 100% alcohol in turn to the glass slide Dehydration of microbial samples on the chip;
[0031] 4) Add 10 μl of the combined gene probe solution to the water sample on the glass slide, add a hybridization solution containing 5.5M NaCl and 40% formamide, and hybridize at a reaction temperature o...
Embodiment 3
[0035] Example 3: Detection of SRPs in the outflow sample of Dongxin Oil Production Plant of Shengli Oilfield
[0036] 1) Prepare the combined gene probe solution, and make the combined gene probe solution with the relative ratio of 65% and 35% of the two probes IV and VI of the present invention;
[0037] 2) Take a water sample from the oil field drainage, and fix the microbial cells including SRPs in the water sample with a concentration of 10% isopolyaldehyde solution;
[0038] 3) Take 1 μl of the above-mentioned fixed microbial cell water sample and drop it into a glass slide, dry the fixed microbial sample on the glass slide at room temperature, and then use 50%, 80% and 100% alcohol in turn to the glass slide Dehydration of microbial samples on the chip;
[0039]4) Add 1 μl of the combined gene probe solution to the water sample on the glass slide, add a hybridization solution containing 3.5M NaCl and 30% formamide, and hybridize at a reaction temperature of 62° C. for ...
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