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Specific bacterium YPC-TA1 and method for treating waste water
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A YPC-TA1, special effect technology, applied in the field of special microbial bacteria and the use of this strain to treat terephthalic acid chemical wastewater, can solve the problems of high content of miscellaneous bacteria, low content of effective bacteria, complicated process and so on
Inactive Publication Date: 2006-09-13
SINOPEC YANGZI PETROCHEM
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Problems solved by technology
Generally speaking, some have complex processes, many structures, large infrastructure investment, and average effluent quality; some have large sludge output, large land occupation, and high operating costs
One of the main reasons is that the content of effective bacteria in the treatment device is low and the content of miscellaneous bacteria is too high, that is, there is a lack of effective bacteria that can efficiently degrade pollutants in sewage, especially for the efficient degradation bacteria in PTA wastewater.
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Embodiment 1
[0117] Embodiment 1: Strain collection and screening
[0118] Take about 1 g of soil somewhere on the terephthalic acid stockyard, place it in an Erlenmeyer flask containing 50 ml of enriched culture solution I, and cultivate it on a shaker at room temperature for 24 hours, and the pH is 7.
[0119] In the second step, the bacterial suspension obtained in the first step is diluted to 10 -5 ~10 -6 , spread on the selection medium, and cultivate for 48 hours to acclimatize.
[0121] Measure its COD and record it as the initial COD.
[0122] The fourth step is to pick out 18 colonies grown on the screening medium prepared in the second step, transfer them into 18 flasks each containing 50ml of enrichment culture solution II, and culture them on a shaker at room temperature for 24 hours.
[0123] The fifth s...
Embodiment 2
[0130] Example 2: The strain collection and screening steps and the method for treating chemical wastewater containing aromatic carboxylic acid are the same as in Example 1, except that benzoic acid is used instead of terephthalic acid. Culture temperature: 25°C; pH 5.5.
Embodiment 3
[0131] Embodiment 3, the strain collecting and screening steps and the method for processing the chemical wastewater containing aromatic carboxylic acid are the same as in Example 1, the difference is that:
[0132] Culture temperature: 35°C; pH 9;
[0133] Prepare simulated wastewater, Na 2 HPO 4 0.05g, (NH 4 ) 2 SO 4 0.lg, the concentration of terephthalic acid is 7000mg / L, with Na 2 CO 3 Adjust the pH to 9 and add distilled water to 100ml. Sterilize at high temperature, insert activated high-efficiency degrading bacteria according to the inoculation amount of 0.5g / L, shake flask culture at room temperature, after 96 hours of degradation, the terephthalic acid is 134mg / L.
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Abstract
The specific bacteria strain for degrading aromatic carboxylic acid, especially phthalic acid, in waste water is YPC-TA1, Delftia tsuruhatensis with preservation number of CGMCC No. 1203. The bacteria strain is obtained from the stockyard containing aromatic carboxylic acid and cultured at 25-35 deg.c and pH 5.5-9 with aromatic carboxylic acid as carbon source. The process of utilizing YPC-TA1 to degrading aromatic carboxylic acid in waste water includes the steps of: adding nutritious salt Na2HPO4 and (NH4)2SO4, regulating pH to 5.5-9, adding water in one time, high temperature sterilization, inoculating activated efficient degrading bacteria in 0.5 g / L, aeration at room temperature to culture for 20-25 hr.
Description
technical field [0001] The invention relates to a special-effect microbial bacterium and a method for treating terephthalic acidchemical industrywastewater by using the bacterium. Background technique [0002] Terephthalic acid (hereinafter referred to as PTA) is one of the important products of petrochemical enterprises, and the corresponding wastewaterdischarge is quite large, and the COD in the wastewater is as high as several thousand or even tens of thousands. Since most of the pollutants in PTA wastewater are aromatic compounds, PTA is also a kind of toxic pollutant, and the treatment of wastewater is difficult, which belongs to wastewater that is difficult to biodegrade. Therefore, it is of great significance to study and develop efficient and cheap large-scale industrial degradation technology and equipment for PTA wastewater treatment. [0003] Various existing technologies for treating PTA wastewater have certain defects and deficiencies. Generally speaking, s...
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