Medicinal composition its preparation method and its use in preparing medicine for treating SARS and its complication
A composition and drug technology, applied to a pharmaceutical composition, its preparation method and its use, can solve the problems of low ganglioside content and low activity
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Embodiment 1
[0115] step 1):
[0116] Take 10kg of fresh rabbit muscle, add 20L of water to homogenate, refrigerate at 4°C for 12 hours, filter with 80-mesh nylon cloth, add 0.1% mycoprotease to the filtrate, adjust the pH value to 7.4, and incubate at 45°C for 2.5 hours. The hydrolyzate was centrifuged at 3000 rpm for 20 minutes. The centrifuged supernatant was filtered through a 0.3um filter membrane, and the filtrate was concentrated under reduced pressure to 800ml, which was used as a rabbit muscle extract for future use, wherein the polypeptide content was 8mg / ml.
[0117] Step (2):
[0118] Get 10kg of fresh bovine brain and homogenate with 40L acetone, filter the homogenate with filter paper to obtain 2500g of filter residue, extract the filter residue three times with 10L of chloroform-methanol (1:1) mixture, filter respectively and combine the filtrates, use a rotary evaporator, Evaporate to dryness under reduced pressure at 37°C to obtain 1.25 g of dry matter. The dry matter w...
Embodiment 2
[0129] step 1):
[0130] Take 10kg of fresh deer muscle, add 16L of water to homogenate, refrigerate at 6°C for 14 hours, filter with 80 mesh nylon cloth, add 0.05% mycoprotease to the filtrate, adjust the pH value to 7.2, and keep it warm at 39°C for 4 hours. The hydrolyzate was centrifuged at 3000 rpm for 20 minutes. The centrifuged supernatant was filtered through a 0.3um membrane filter, and the filtrate was concentrated under reduced pressure to 800ml, wherein the polypeptide content was 2.725mg / ml, which was used as deer muscle extract for future use.
[0131] Step (2):
[0132] Take 10kg of fresh bovine brain and homogenate with 35L acetone, filter the homogenate with filter paper to obtain 2500g of filter residue, extract the filter residue three times with 7.5L of chloroform-methanol (5:1) mixture, filter respectively and combine the filtrates, use a rotary evaporator, Evaporate to dryness under reduced pressure at 37°C to obtain 1.25 g of dry matter. The dry matte...
Embodiment 3
[0142] step 1):
[0143] Take 10 kg of fresh rabbit muscle, add 25 L of water to homogenate, refrigerate at 8°C for 16 hours, filter with 80-mesh nylon cloth, add 0.5% mycoprotease to the filtrate, adjust the pH value to 7.6, and incubate at 42°C for 2 hours. The hydrolyzate was centrifuged at 3000 rpm for 20 minutes. The centrifuged supernatant was filtered through a 0.3um membrane filter, and the filtrate was concentrated under reduced pressure to 800ml, in which the polypeptide content was 5mg / ml, which was used as a rabbit muscle extract for future use.
[0144] Step (2):
[0145] Get 10kg of fresh horse brain and homogenate with 30L acetone, filter the homogenate with filter paper to obtain 2500g of filter residue, extract the filter residue three times with 12.5L of chloroform-methanol (20:1) mixture, filter respectively and combine the filtrates, use a rotary evaporator, Evaporate to dryness under reduced pressure at 37°C to obtain 1.25 g of dry matter. The dry matte...
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