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Process for preparation optical pure (R)-2- octanol by microorganism and its special microorganism

A microbial method and optical technology, applied in the field of biological separation of racemic compounds

Inactive Publication Date: 2006-11-22
JIANGNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0013] Chinese patent CN1366550A has reported a kind of (R)-2-octanol dehydrogenase, which can preferentially oxidize (R)-2-octanol in the two optical isomers of 2-octanol, but it cannot Racemic 2-octanol is converted as a substrate to obtain (R)-2-octanol, and in the application of chiral alcohol preparation, only ketone is used as a substrate to reduce the corresponding chiral alcohol, rather than racemate as Substrates undergo a process of chiral resolution to yield optically pure products

Method used

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  • Process for preparation optical pure (R)-2- octanol by microorganism and its special microorganism

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Cultivation of bacterial strains: the composition of the medium is that the components contained in each 100ml of culture solution are in g: glucose 2-6, yeast extract 0.25-1, (NH 4 ) 2 HPO 4 0.5~2.0, KH 2 PO 4 0.1~0.5, MgSO 4 ·7H 2 O 0.02~0.06, NaCl 0.0025~0.01, ZnSO 4 ·7H 2 O 0.001~0.005, FeSO 4 ·7H 2 O 0.001~0.005, CuSO 4 ·5H 2 O 0.0001~0.0005, MnSO 4 4H 2 O 0.00025~0.001.

[0060] The culture conditions are as follows: the initial pH is 6.0-8.0, the filling volume is 30%, the culture temperature is 25° C., the rotation speed of the shaking flask is 100-300 rpm, and the culture time is 72 hours.

Embodiment 2

[0062] Cultivation of the bacterial strain: the composition of the culture medium is the same as in Example 1. The culture conditions are as follows: the initial pH is 6.0-8.0, the filling volume is 5%, the culture temperature is 35° C., the rotation speed of the shaking flask is 100-300 rpm, and the culture time is 24 hours.

Embodiment 3

[0064] Preparation of whole cells: Take a loop of C.cylindracea CCTCC M204041 from the slope and inoculate it in a 250ml shaker flask with 30% culture solution at 25°C and 100-300rpm for 72 hours; The body was centrifuged and washed twice with saline, and the whole cells of the strain were collected for transformation reaction.

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Abstract

The invention discloses a method for preparing optically pure (R)-2-octanol by a microbial method and a special microorganism thereof, belonging to the technical field of separating racemic compounds by a biological method. The present invention obtains the strain Candida cylindracea SYB-3 CCTCC M204041 through screening. The bacterial strain is cultivated and prepared from whole cells, and racemic 2-octanol is used as a substrate to carry out the catalytic conversion reaction of microbial cells, and the product (R)-2-octanol is obtained by asymmetric conversion of racemic 2-octanol. The optical purity reaches above 90% e.e. The present invention determines the reaction system and reaction conditions for the asymmetric transformation of racemic 2-octanol in the whole cell of the strain, and the optical purity of the product is improved; the present invention also helps to understand the biological diversity of racemic compound resolution , which is of great significance for the development of special enzyme sources for resolution and the research of resolution methods in the future.

Description

technical field [0001] The invention discloses a method for preparing optically pure (R)-2-octanol by a microbial method and a special microorganism thereof, belonging to the technical field of separating racemic compounds by a biological method. Background technique [0002] The chemical structure of 2-octanol is: [0003] [0004] Racemic 2-octanol consists of two enantiomers (R)-2-octanol and (S)-2-octanol. [0005] Optically pure 2-octanol, including (R)-2-octanol and (S)-2-octanol, is not only an anabolic steroid, vitamin E, and insect pheromones (bioinsecticides) but also many optically active drugs and It is an important chiral intermediate of pesticides, and is an indispensable and important chiral raw material for the preparation of high-performance liquid crystals and their devices. Its optical activity and high dielectric constant are closely related to many properties of liquid crystals; as an important intermediate, it is also Used in organic synthesis of f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/04C12R1/72
Inventor 徐岩聂尧穆晓清
Owner JIANGNAN UNIV
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