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Usage of glanthidine, total alkali extract and preparation method and usage thereof

A technology of extract and lycorine, applied in the direction of medical preparations containing active ingredients, drug combinations, pharmaceutical formulas, etc., can solve the problem of lack of SARS virus-specific formulas and drugs

Inactive Publication Date: 2006-12-20
辉源生物科技(上海)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Although many traditional Chinese medicine preparations and formulas that are said to have anti-SARS effects have been announced, there is still a lack of formulas and drugs that have a targeted effect on the SARS virus

Method used

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  • Usage of glanthidine, total alkali extract and preparation method and usage thereof
  • Usage of glanthidine, total alkali extract and preparation method and usage thereof
  • Usage of glanthidine, total alkali extract and preparation method and usage thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Extraction and detection of embodiment 1 Lycoris extract

[0054] 1. Materials and Instruments

[0055] Materials: Purchased in Anguo, Anhui in November 2001, identified by Professor Yan Yuning of Beijing University of Traditional Chinese Medicine and numbered for storage.

[0056] instrument:

[0057] Soxhlet extractor (common Chinese type); Pulverizer (Beijing Guangming Instrument Factory FZ-102); Rotary evaporator (Shanghai Shensheng Instrument Company); Four-hole water bath (Beijing Instrument Factory); absolute ethanol (analytical grade, Chemical Reagent Factory); Chloroform (analytical grade, Beijing Chemical Reagent Factory); electronic balance (accurate 0.1g, Beijing Medical Balance Factory DT-500); experimental filter paper.

[0058] 2. Steps:

[0059] Get 143g Lycoris medicinal material, crush it with a pulverizer, and put it into a paper tube.

[0060] Put 800ml of chloroform into the flat-bottomed flask in the Soxhlet extractor, put the prepared lycoris ...

Embodiment 2

[0066] Example 2 Lycoris extract anti-SARS activity test

[0067] Materials and methods

[0068] Test drug: Lycoris extract prepared in Example 1.

[0069] Cells: Vero cells (Vero).

[0070] Virus: SARS virus Beijing No. 1 (BJ-01).

[0071] Reagents: DMEM medium (product of GIBCO), streptomycin, L-glutamine (Invitrogen, USA), fetal bovine serum (Hyclone, USA), Hepes, MTS (Promega, USA), PMS, penicillin (Sigma company), sodium bicarbonate (purchased from Jingke Chemical Reagent Company and imported).

[0072] Cell culture medium and reagent preparation: DMEM culture medium: containing 10% newborn fetal bovine serum, 3% L-glutamine, 1% penicillin, streptomycin, 7.5% sodium bicarbonate, 10mM Hepes;

[0073] MTS / PMS staining solution: prepared according to the operating instructions of Promega Company.

[0074] MTS: formazan compound [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethylcarboxylate)-2-(4-sulfophenyl)-2H-tetrazole (gold Ong)

[0075] PMS: Phenazine Dimethyl Sulfat...

Embodiment 3

[0096] The extraction and detection of embodiment 3 Lycoris total alkali extract

[0097] Experimental steps:

[0098] Get a certain amount of Lycoris extract obtained in Example 1 and dissolve it in a 0.1mol / L HCl solution, and shake it to fully dissolve it.

[0099] The acid-containing solution is filtered by vacuum pump to remove the residue.

[0100] Filtrate with NaHCO 3 Adjust the acidic solution to make PH=9-10. And use CHCL 3 Extract three times. CHCL 3 The solution.

[0101] Recovery of CHCL 3 And spin-dried to obtain the total alkali powder, which was used for the determination of the effective part in the next step without purification.

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Abstract

The present invention discloses the use of lycoramine in preparing medicine for resisting SARS virus, total lycoramine extract containing lycoramine and its preparation process, use in preparing medicine for resisting SARS virus and the composition therewith.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicine, in particular to an extract of lycoris and its application. The present invention also relates to compositions containing Lycoris extract. Background technique [0002] Lycoris radiatae (Bulbus Lycoridis Radiatae), also known as old crow garlic, garlic grass, and cockroach flower, is derived from the bulb of Lycoris radiata (L’Herit.) Herb., a plant of the Amaryllidaceae family. It is born in damp mountains or under jungles, and is also cultivated. Produced in East China, Central South and Southwest China. The chemical composition contains lycorine, galanthamine, lycora-mine, etc. Warm in nature, spicy and sweet in the mouth. Cure mainly detumescence, detoxification, emetic. It is used for boils and swollen toxins, food poisoning, phlegm and saliva congestion, jaundice, edema and ascites. At present, the research on its components is mainly focused on saponins and weak acid and w...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/407A61K31/4375A61P31/14A61P11/00C07D491/04
Inventor 王慧郭海燕栗世铀王丽娟张以琳马良会谈学海
Owner 辉源生物科技(上海)有限公司