Method of preparing microsphere of ethoxyl copolymer PLGA in interferon poly acid
A technology of polylactic acid and interferon, applied in medical preparations with non-active ingredients, medical preparations containing active ingredients, drug combinations, etc., can solve the problems of short biological half-life, poor stability, hindering use, etc., and achieve smooth surface , Good fluidity, prolonging the effect of action time
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[0025] Example 1 Preparation of interferon polylactic acid-glycolic acid copolymer (PLGA) microspheres
[0026] (1) Preparation of polylactic acid-glycolic acid copolymer (PLGA) solution
[0027] 400 mg of polylactic acid-glycolic acid copolymer (PLGA) was dissolved in 2 ml of dichloromethane to form an organic phase (20% g / ml).
[0028] (2) Preparation of interferon PBS solution
[0029] Dissolve 40 mg of lyophilized recombinant human interferon-α for injection in 300 μl of 1% (g / ml) gelatin solution to form an internal aqueous phase;
[0030] (3) Preparation of colostrum
[0031] Inject the internal water phase into the organic phase, stir and emulsify for 1 minute at 9000 rpm to form colostrum (W / O);
[0032] (4) Preparation of double emulsion
[0033] Add the colostrum to 300ml solution (outer water phase) containing 1% (g / ml) PVA and 1% (g / ml) NaCl under stirring at 2000 rpm, emulsify for 10 minutes, and emulsify into double emulsion (W / O / W);
[0034] (5) Formation of micros...
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[0039] Example 2 Preparation of interferon polylactic acid-glycolic acid copolymer (PLGA) microspheres
[0040] (1) Preparation of polylactic acid-glycolic acid copolymer (PLGA) solution
[0041] 600 mg of polylactic acid-glycolic acid copolymer (PLGA) was dissolved in 2 ml of dichloromethane to form an organic phase (30% g / ml).
[0042] (2) Preparation of interferon PBS solution
[0043] Dissolve 60 mg of freeze-dried recombinant human interferon-α for injection in 300 μl of 30% PEG400 solution to form an internal aqueous phase;
[0044] (3) Preparation of colostrum
[0045] Inject the internal water phase into the organic phase, stir and emulsify for 3 minutes at 1000 rpm to form colostrum (W / O);
[0046] (4) Preparation of double emulsion
[0047] Add the colostrum to 200ml containing 3% (g / ml) PVA and 0.5% (g / ml) NaCl solution (outer water phase) under stirring at 1600rpm, emulsify for 20 minutes, and emulsify into double emulsion (W / O / W);
[0048] (5) Formation of microspher...
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