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Method of preparing microsphere of ethoxyl copolymer PLGA in interferon poly acid

A technology of polylactic acid and interferon, applied in medical preparations with non-active ingredients, medical preparations containing active ingredients, drug combinations, etc., can solve the problems of short biological half-life, poor stability, hindering use, etc., and achieve smooth surface , Good fluidity, prolonging the effect of action time

Inactive Publication Date: 2007-04-04
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the short biological half-life and poor stability of peptide and protein drugs, they are easily metabolized by enzymes in the body and difficult to absorb in the gastrointestinal tract, so long-term and frequent injections are required, which hinders their convenient, extensive and effective clinical application. use

Method used

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  • Method of preparing microsphere of ethoxyl copolymer PLGA in interferon poly acid
  • Method of preparing microsphere of ethoxyl copolymer PLGA in interferon poly acid

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Embodiment 1 Preparation of interferon poly(lactic-co-glycolic acid) (PLGA) microspheres

[0026] (1) Preparation of polylactic acid-glycolic acid copolymer (PLGA) solution

[0027] 400 mg of poly(lactic-co-glycolic acid) (PLGA) was dissolved in 2 ml of dichloromethane to form an organic phase (20% g / ml).

[0028] (2) Preparation of interferon PBS solution

[0029] Dissolve 40 mg of lyophilized recombinant human interferon-α for injection in 300 μl of 1% (g / ml) gelatin solution to form an internal aqueous phase;

[0030] (3) Preparation of colostrum

[0031] Inject the inner aqueous phase into the organic phase, stir and emulsify at 9000rpm for 1 minute to form colostrum (W / O);

[0032] (4) Preparation of double emulsion

[0033] Add colostrum to 300ml containing 1% (g / ml) PVA and 1% (g / ml) NaCl solution (outer water phase) under the situation of stirring at 2000rpm, emulsify for 10 minutes, emulsify into double emulsion (W / O / W);

[0034] (5) Formation of microsp...

Embodiment 2

[0039] Embodiment 2 Preparation of interferon poly(lactic-co-glycolic acid) (PLGA) microspheres

[0040] (1) Preparation of polylactic acid-glycolic acid copolymer (PLGA) solution

[0041] 600 mg of poly(lactic-co-glycolic acid) (PLGA) was dissolved in 2 ml of dichloromethane to form an organic phase (30% g / ml).

[0042] (2) Preparation of interferon PBS solution

[0043] Dissolve 60 mg of lyophilized recombinant human interferon-α for injection in 300 μl of 30% PEG400 solution to form an inner aqueous phase;

[0044] (3) Preparation of colostrum

[0045] Inject the inner aqueous phase into the organic phase, stir and emulsify for 3 minutes at 1000rpm to form colostrum (W / O);

[0046] (4) Preparation of double emulsion

[0047] Add colostrum to 200ml containing 3% (g / ml) PVA and 0.5% (g / ml) NaCl solution (outer water phase) under the situation of stirring at 1600rpm, emulsify for 20 minutes, emulsify into double emulsion (W / O / W);

[0048] (5) Formation of microspheres ...

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Abstract

A microspherical interferon polylactate-glycollate copolymer (PLGA) is prepared from the polylactate-glycollate copolymer (PLGA) as microspherical carrier and the recombinant human interferon-alpha as the coated object by remulsifying-solvent evaporating method.

Description

technical field [0001] The invention relates to a method for preparing biodegradable controlled-release microspheres of bioactive drugs, and belongs to the field of cross research between biomedical polymer materials and controlled-release preparations of bioactive drugs. Background technique [0002] In recent years, with the rapid development of biotechnology, more and more peptide and protein drugs have been developed, and have shown many unique curative effects in clinical applications, such as insulin, hepatitis B vaccine, interferon, etc. However, due to the short biological half-life and poor stability of peptide and protein drugs, they are easily metabolized by enzymes in the body and difficult to absorb in the gastrointestinal tract, so long-term and frequent injections are required, which hinders their convenient, extensive and effective clinical application. use. [0003] Recombinant human interferon-α is a product prepared by biotechnology. It is a milky white p...

Claims

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Application Information

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IPC IPC(8): A61K38/21A61K9/14A61K47/34A61P37/02A61P35/00A61P31/12
Inventor 徐安龙杨帆高建萍赵耀明王磊董美玲
Owner SUN YAT SEN UNIV
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