Wheat anti-powdery mildew gene Pm21 linked codorminant PCR marker and its usage

A powdery mildew resistance gene, wheat technology, applied in genetic engineering, plant genetic improvement, application, etc., can solve the problem of being in a homozygous or heterozygous state and cannot be known.
CN1322133CInactive Publication Date: 2007-06-20NANJING AGRICULTURAL UNIVERSITY

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NANJING AGRICULTURAL UNIVERSITY
Publication Date
2007-06-20
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a wheat anti-powdery mildew gene Pm21 linked codorminant PCR marker and its use, wherein disease-resistant gene analogues Ta-LRR2 sequence design primers NAU / XiBao 16F and NAU / XiBao 16R are obtained through suppression subtractive hybridization between Yangmai No.5 and translocation lines, a series of material tests have shown that, the label NAU / XiBao16 is interlocked with translocation lines 6VS / 6AL powdery mildew resistant gene Pm21, and one PCR experiment can amplify two specific belts from common wheat 6AS and Haynaldia Vollosa 6VS chromosomes simultaneously, the label NAU / XiBao16 can be used for the molecular label auxiliary selection in wheat powdery mildew disease resistant breeding, and for the purity identification of common wheat seeds.
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Description

1. Technical field

[0001] The invention discloses a marker linked with wheat powdery mildew resistance gene Pm21, which can be used for molecular marker-assisted selection in wheat powdery mildew resistance breeding; the marker can also be used to identify the purity of common wheat seeds, especially to identify those created by cell engineering and chromosome engineering Materials involving mutations in chromosome 6AS of homolog group VI of common wheat. 2. Technical background

[0002] Wheat (T. aestivum L), as an important food crop worldwide, plays a pivotal role in agricultural production, but its production is affected by many biotic and abiotic stresses. Wheat powdery mildew (Ergsiphe greminis f.sp.tritici), caused by the obligate parasitic fungus Erysiphegraminis DC, is one of the diseases that are increasingly harmful to wheat production in China and many countries in the world. improvement, and there has been an increasing trend in recent years.

[0003] Common w...

Claims

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