Wheat anti-powdery mildew gene Pm21 linked codorminant PCR marker and its usage
A powdery mildew resistance gene, wheat technology, applied in genetic engineering, plant genetic improvement, application, etc., can solve the problem of being in a homozygous or heterozygous state and cannot be known.
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[0038] 1. Screening for disease resistance gene analogues located on the 6VS chromosome
[0039] The powdery mildew resistant 6VS / 6AL translocation line was backcrossed with Yangmai 5 for 7 generations, and a translocation line with a background close to that of Yangmai 5 was obtained. The translocation line and the parent Yangmai 5 were cultivated in pots, and used The cover made of transparent film is isolated to prevent external bacteria from polluting. When the plants reached the second-leaf stage, the translocation line was inoculated with the powdery mildew mixed strain by shaking off, and induced for 6 hours and 12 hours respectively, while Yangmai 5 was not induced by the powdery mildew. Then extract the RNA from the leaves of the induced translocation line and the leaves of Yangmai 5 without induction, and construct the forward suppressive subtraction hybridization (SSH) library with the RNA of the translocation line as the Tester and the RNA of Yangmai 5 as the Drive...
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