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Process for separating and purifying ebormycine from fermented myxobacterium liquid

A technology of epothilone and myxobacteria, which is applied in the field of separation and purification of macrolide compounds, can solve the problems of high process cost, failure to achieve industrial production, and no major progress patents, etc., to improve feasibility and reliability Scalability, good economic benefits and application prospects, efficient and economical separation and purification effects

Inactive Publication Date: 2002-09-18
SHANDONG UNIV
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  • Summary
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Problems solved by technology

Our research shows that this method has certain defects and deficiencies, especially the inability to meet the various requirements of industrial production
Mainly manifested in: the steps are too complicated, so that the efficiency of the operation is low and the process cost is very high; the yield of the product is low, no more than 30%, and the process loss is as high as 70%; during resin adsorption, use Amberlite (Amberlite) XAD -16 resin, the cost is very high, and there is no resin substitute of the same model in China at present; To make epothilone A and B reach the drug purity (greater than 90%), it must be separated through high-efficiency liquid phase, which is in industrial production is difficult to achieve
After searching the literature and patents, there is no major progress and related patents in this direction at home and abroad

Method used

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  • Process for separating and purifying ebormycine from fermented myxobacterium liquid
  • Process for separating and purifying ebormycine from fermented myxobacterium liquid
  • Process for separating and purifying ebormycine from fermented myxobacterium liquid

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Embodiment 2

[0043] In the method of the present invention, the adsorption rate of the mixed resin for epothilones is 100%, and when the crystallization step is completed, the extraction rate of epothilones is 90%, and the purity is 97%. The extraction rate of bleomycin A and B is 89%, and the purity is 99.99%. Embodiment 2: The present invention utilizes technical means such as mixed resin adsorption, solid-liquid fractional extraction, molecular sieve chromatography, crystallization and high-efficiency liquid phase separation, separates and extracts epothilone from a kind of myxobacterium S. cellulosus fermentation broth, Concrete step sequence is as follows: (1) the preparation of mixed resin: CD180, CAD-40, XDA, S-8, NKA-II, AB-8 six kinds of resins are by weight 0.8: 0.8: 1.8: 0.8: 2.8: 1.8 Mix, suspend and dissolve in an equal volume of distilled water to prepare a mixed resin solution. (2) Mixed resin adsorption: when carrying out S. cellulosus fermentation, add 1% (volume / volume) ...

Embodiment 3

[0047] In the method of the present invention, the adsorption rate of the mixed resin for epothilones is 95%, and when the crystallization step is completed, the extraction rate of epothilones is 90%, and the purity is 95%. The extraction rate of bleomycin A and B is 82%, and the purity is 99.91%. Embodiment 3: The present invention utilizes technical means such as mixed resin adsorption, solid-liquid stepwise extraction, molecular sieve chromatography, crystallization and high-efficiency liquid phase separation, separates and extracts epothilone from a kind of myxobacterium S. cellulosus fermentation broth, Concrete step sequence is as follows: (1) the preparation of mixed resin: CD180, CAD-40, XDA, S-8, NKA-II, AB-8 six kinds of resins are by weight 1.2: 1.2: 2.3: 1.2: 3.2: 2.3 Mix, suspend and dissolve in an equal volume of distilled water to prepare a mixed resin solution. (2) Mixed resin adsorption: when carrying out S. cellulosus fermentation, add 1% (volume / volume) mix...

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Abstract

A process for extracting Epophilone from fermented liquid of myxobacterium includes mixed resin adsorption, solid and liquid extractions, molecular sieve chromatography, crystallizing and effiicent liquid-phase separation. Its advantages are high purity and extraction rate (more than 80%). The said Epophilone is an antineoplastic compound.

Description

(1) Technical field [0001] The invention relates to a method for separating and purifying macrolide compounds from myxobacteria fermentation liquid, in particular to a method for separating and purifying epothilone from myxobacteria fermentation liquid. (2) Background technology [0002] At present, the most successful clinical anti-tumor chemotherapy drug is paclitaxel, a natural compound that promotes microtubule polymerization.  ) and its analogs Taxotere (docetaxel, Taxotere  ), has been used in the treatment of solid cancers such as ovarian cancer, thymus cancer, colon cancer, lung cancer and liver cancer. The success of the inducible anti-tumor drug paclitaxel and its shortcomings in chemotherapy (low water solubility and cell drug resistance during chemotherapy, etc.) have prompted researchers to further screen for drugs with better chemical properties, biological properties and pharmacology. properties of microtubule stabilizers. However, after a long time and a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D305/14C07D417/06
Inventor 胡玮李越中刘新利韩冠君
Owner SHANDONG UNIV
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