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Chloromycetin enzyme immunoassay kit for animal food and its immunoassay method

A detection kit, chloramphenicol technology, applied in the field of enzyme immunoassay to achieve the effect of simple sample pretreatment

Inactive Publication Date: 2003-01-08
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Several types of chloramphenicol enzyme immunoassay kits have been introduced to the market in foreign countries and have been reported in literature, but there is no report of such kits on the market in China.

Method used

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  • Chloromycetin enzyme immunoassay kit for animal food and its immunoassay method
  • Chloromycetin enzyme immunoassay kit for animal food and its immunoassay method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The monoclonal CAP antiserum prepared according to the aforementioned method was coated on the microtiter plate and placed in a refrigerator at 4°C overnight.

[0047] 1. According to the above method, carry out sample processing.

[0048] 2. Reagent preparation.

[0049] 1) Standard CAP reagent: add 20ml of B reagent (diluent) to CAP and mix well.

[0050] 2) Enzyme-labeled antigen reagent: Dissolve C reagent plus 10ml D reagent (enzyme-labeled antigen diluent), and mix well.

[0051] 3) Preparation of washing solution E: add 200 ml of distilled water to the reagent E containing 0.1% Tween-20 (Tween-20) phosphate (PBS) to prepare washing solution E, which is used for washing the microtiter plate.

[0052]4) Substrate mixture preparation: according to the required amount each time, use F reagent (o-phenylenediamine) and G reagent (sodium acetate-citric acid buffer) in the ratio of G:F=7-9:3-1 Dilute reagent F, and then add 14 μl of hydrogen peroxide reagent H to each...

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Abstract

This invention relates to CAP enzyme immunity test reagent box and ELISA competition method to test CAP. To use CAP and BSA coupler as the CAP antigen immunity small mouse, the monocolnal CAP antiserum with CAP antibody is obtained which is then put on the enzyme specimen board after separation, purification and dilution to be warm cultivated, washed and added with sample diluent and enzyme standard antigen to be reacted and washed to be added with enzyme substrate to display color with easy pretreatment especially for testing remained CAP of meat food stuffs.

Description

technical field [0001] The invention discloses a chloramphenicol (chlora mphenicol, CAP) enzyme immunoassay kit and a detection method thereof in animal food, belonging to the technical field of enzyme immunoassay. Background technique [0002] Chloramphenicol is a broad-spectrum antibiotic that has been widely used in the treatment of various infectious diseases in animals. However, chloramphenicol has serious side effects. It can inhibit the hematopoietic function of human bone marrow and cause aplastic anemia and agranulocytosis disease and other diseases, so chloramphenicol residues in animal foods pose a potential hazard to human health. The United States and the European Community have strict restrictions on the use of chloramphenicol in animals and the amount of chloramphenicol residues in animal foods . my country has not yet formulated food hygiene standards for chloramphenicol residues. Due to the broad-spectrum antibacterial effect of chloramphenicol, good effec...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N33/535G01N33/543G01N33/577
Inventor 胥传来丁宏标李相前邵蔚蓝徐保国詹斌
Owner JIANGNAN UNIV
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