Chloromycetin enzyme immunoassay kit for animal food and its immunoassay method

A detection kit, chloramphenicol technology, applied in the field of enzyme immunoassay to achieve the effect of simple sample pretreatment

Inactive Publication Date: 2003-01-08
JIANGNAN UNIV
View PDF0 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Several types of chloramphenicol enzyme immunoassay kits have been introduced to the market in foreign co

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Chloromycetin enzyme immunoassay kit for animal food and its immunoassay method
  • Chloromycetin enzyme immunoassay kit for animal food and its immunoassay method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The monoclonal CAP antiserum prepared according to the aforementioned method was coated on the microtiter plate and placed in a refrigerator at 4°C overnight.

[0047] 1. According to the above method, carry out sample processing.

[0048] 2. Reagent preparation.

[0049] 1) Standard CAP reagent: add 20ml of B reagent (diluent) to CAP and mix well.

[0050] 2) Enzyme-labeled antigen reagent: Dissolve C reagent plus 10ml D reagent (enzyme-labeled antigen diluent), and mix well.

[0051] 3) Preparation of washing solution E: add 200 ml of distilled water to the reagent E containing 0.1% Tween-20 (Tween-20) phosphate (PBS) to prepare washing solution E, which is used for washing the microtiter plate.

[0052]4) Substrate mixture preparation: according to the required amount each time, use F reagent (o-phenylenediamine) and G reagent (sodium acetate-citric acid buffer) in the ratio of G:F=7-9:3-1 Dilute reagent F, and then add 14 μl of hydrogen peroxide reagent H to each...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

This invention relates to CAP enzyme immunity test reagent box and ELISA competition method to test CAP. To use CAP and BSA coupler as the CAP antigen immunity small mouse, the monocolnal CAP antiserum with CAP antibody is obtained which is then put on the enzyme specimen board after separation, purification and dilution to be warm cultivated, washed and added with sample diluent and enzyme standard antigen to be reacted and washed to be added with enzyme substrate to display color with easy pretreatment especially for testing remained CAP of meat food stuffs.

Description

technical field [0001] The invention discloses a chloramphenicol (chlora mphenicol, CAP) enzyme immunoassay kit and a detection method thereof in animal food, belonging to the technical field of enzyme immunoassay. Background technique [0002] Chloramphenicol is a broad-spectrum antibiotic that has been widely used in the treatment of various infectious diseases in animals. However, chloramphenicol has serious side effects. It can inhibit the hematopoietic function of human bone marrow and cause aplastic anemia and agranulocytosis disease and other diseases, so chloramphenicol residues in animal foods pose a potential hazard to human health. The United States and the European Community have strict restrictions on the use of chloramphenicol in animals and the amount of chloramphenicol residues in animal foods . my country has not yet formulated food hygiene standards for chloramphenicol residues. Due to the broad-spectrum antibacterial effect of chloramphenicol, good effec...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/53G01N33/535G01N33/543G01N33/577
Inventor 胥传来丁宏标李相前邵蔚蓝徐保国詹斌
Owner JIANGNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap