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Process for preparing citric acd by fermentation and bacterial strain for it

A technology of citric acid and fermentation temperature, which is applied in the production of citric acid by fermentation. The field of strains used in this method can solve the problems of low fermentation level and difficulties, and achieve the effects of saving production costs and shortening the production cycle

Inactive Publication Date: 2003-01-29
上海新立工业微生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since molasses contains various organic substances and inorganic substances with very complex components, it is still very difficult to use molasses as a raw material to directly ferment and produce citric acid in the prior art.
In response to this problem, some people use pretreated molasses as fermentation raw material, but this method also needs to carry out additional pretreatment steps on molasses, and the fermentation level is not high, and various indicators such as acid production and conversion rate cannot be compared with Compared with the process of hydrolyzing sugar as raw material and my country's process of using starch as raw material
There are no reports on the direct fermentation of citric acid from untreated molasses

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0017] a) Composition of medium

[0018] Slant culture medium: 6% sugar (for molasses containing 50% reducing sugar (purchased from Sichuan Qianwei Sugar Factory), 12% molasses); 2.5% agar.

[0019] Plate medium: 6% sugar (12% molasses); 2.5% agar.

[0020] Acid medium: 6% sugar (12% molasses); 2% citric acid; 3.0% agar.

[0021] Fermentation medium: 15% sugar (30% molasses); 0.3% ammonium sulfate.

[0022] b) Mutagenesis and screening of Aspergillus niger SNGB313

[0023] First, the wild-type Aspergillus niger (Aspergillus . niger) (named 13-1) was isolated from the orchard soil in Fenghua, Zhejiang. Subsequently, UV treatment is carried out. The specific method is as follows: take one of the slopes, wash and scrape with sterile water, place the washed spores in a glass bead-triangle flask and shake for 5 minutes, and dilute the supernatant to a certain concentration. Take 2 ml of the spore suspension in a petri dish, and shake it for 15-20 minutes under a 40W ultraviole...

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Abstract

A process for preparing citric acid by fermentation features that the molasses used as raw material is fermented by Aspergillus niger SNGB313.

Description

technical field [0001] The invention relates to the field of biological fermentation, in particular to the field of fermentative production of organic acids. More specifically, the present invention relates to a method for fermenting and producing citric acid with molasses as a raw material, and the bacterial strain used in the method. Background technique [0002] Citric acid is usually produced by fermentation using starch as a carbon source. Since my country has a well-developed sugar industry, the source of molasses as a by-product of the sugar industry is quite rich, so some people try to use molasses as a carbon source to ferment and produce citric acid. However, since molasses contains various organic substances and inorganic substances with very complex components, it is still very difficult to use molasses as a raw material to directly ferment and produce citric acid in the prior art. In response to this problem, some people use pretreated molasses as fermentation...

Claims

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Application Information

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IPC IPC(8): C12P7/48
Inventor 朱宝娣胡军钱志良
Owner 上海新立工业微生物科技有限公司