Design method for compound amplification of STR primer

A technology of compound amplification and primer design, which is applied in the field of primer design for compound amplification of STR, can solve the problems of cumbersome experimental process, poor reproducibility of results, and reduced PCR amplification efficiency, and achieves simplified experimental process and reproducibility. high sex effect

Inactive Publication Date: 2003-03-12
SICHUAN UNIV
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Problems solved by technology

In this compound amplification method, since the amounts of amplified products corresponding to each pair of primers are not consistent, the relative concentration between each pair of primers must be adjusted, and reactions and competitions between each primer must also occur, thereby greatly reducing The efficiency of PCR amplification
At the same time, other reaction components in the reaction system need to be adjusted, the whole experimental process is cumbersome, and the reproducibility of the results is very poor

Method used

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Examples

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Embodiment Construction

[0027] In this example, the designed primers were used for simultaneous multiplex amplification of the target genes of four loci D1S1612, D17S2196, D20S161 and D6S477 in the same PCR reaction system.

[0028] The designed short primers are: YPA: (5'--ATTTAGGTGACACTATAGAATAC-3')

[0029] YPB: (5′--TAATACGACTCACTATAGGGAGAC-3′)

[0030] The long primers YPA-P1 and YPB-P2 designed for each locus are:

[0031] D1S1612 locus:

[0032] YPA-P1: 5′--ATTTAGGTGACACTATAGAATAC

[0033] TCCCATGCCAAAATTCTTAG-3′

[0034] YPB-P2: 5′--TAATACGACTCACTATAGGGAGAC

[0035] GAAAGAAAGAGAAAGAAGGAAAGG-3′

[0036] D17S2196 locus:

[0037] YPA-P1: 5′--ATTTAGGTGACACTATAGAATAC

[0038] CCAACATCTAGAATTAATCAGAATC-3′

[0039] YPB-P2: 5′--TAATACGACTCACTATAGGGAGAC

[0040] ATATTTCAATATTGTAACCAGTCCC-3′

[0041] D20S161 locus:

[0042] YPA-P1: 5′--ATTTAGGTGACACTATAGAATAC

[0043] C...

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Abstract

A process for designing the primer for the complex amplification of STR includes respectively adding a non-human genom sequence to the terminal 5' of the oligonucleotide primer P1 and P2 able to specifically bind with human genom sequence to obtain long primers YPA-P1 and YPB-P2, using them as the primer pair for the first stage of polymerase chain reaction, and directly using said non-human genom sequence as the primer pair for the second stage of PCR.

Description

technical field [0001] The invention relates to a primer design method for one-time amplification of multiple DNA target fragments in vitro. Background technique [0002] The deoxyribonucleotide (DNA) in the nucleus of the human body is the carrier of genetic information, and it is a double helix structure formed by two long chains of nucleic acid. There are deoxynucleotide units (dNTP) on each long chain of nucleotides, and A, T, G, and C are their bases. The arrangement of the above-mentioned bases controls the expression of genetic information (such as height, weight, intelligence, etc.). The two long strands of nucleic acid are combined by hydrogen bonds, and the principle of base pairing is complementary, that is, A-T, G-C; the complementary double strands have directions, one is from the 5'→3' end, and the other complementary strand is from the 3' →5' end. When somatic cells divide, the DNA in the organism is replicated, and the double helix structure is unwound und...

Claims

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Application Information

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IPC IPC(8): C07H21/04C12P19/34
Inventor 侯一平李英碧应斌武冀强董建国吴谨张霁
Owner SICHUAN UNIV
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