Method for coating nucleic acid with metal by means of non-field produced metal nanoparticles
A metal nanoparticle and metallization technology is applied in the field of metallization of metal nanoparticles for nucleic acid, which can solve problems such as dryness and hybridization ability.
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Embodiment 1
[0054] THP-Au particles were synthesized using extensive gold solution deformation as described by Duff et al. (1993). Stock solutions of THP-Au were diluted with approximately 9 volumes of ethanol and stored at 4°C. This solution was about 1 year old when it was used in this experiment. DNA (from calf thymus) was immobilized in O 2 plasma-treated silicon. Then put enough THP-Au solution to cover the substrate and let it evaporate to dryness (about 10min). Thereafter, the substrate is rinsed with water and dried. figure 1 It is an AFM image, showing particles with a size of about 2 to 5 nm on the DNA, and almost none on the silicon material. Then, the base material was coated with freshly prepared Gold Enhance The solution was treated for 5 minutes, rinsed with water, and dried. AFM image ( figure 2 ) shows a network of enlarged particles. SEM image ( image 3 ) shows an elongated strand of metallized DNA.
Embodiment 2
[0056] The THP-Au particle solution from Example 1 was diluted 6-fold with a solution (pH 7.0) containing 70 mg / l DNA (from calf thymus) and 0.01 M sodium phosphate. The resulting solution was heated in a 50°C water bath for 1 hr, then held at 4°C for 24 hr. The resulting solution was added to a mica substrate and washed with MgCl 2 to process. After 1 min, the resulting substrate was rinsed with water and dried. AFM images showing DNA molecules templated with individual particles ( Figure 4 ).
Embodiment 3
[0058] Contains 0.065 [Au(THP) 4 ] The aqueous solution of Cl adopts the NaAuCl of 1:6 molar ratio 4 Prepared by reaction with THP [Berning et al. (1997), Chemische Berichte / Recueil, Vol. 130, p. 907]. THP is prepared by reacting THPC with one equivalent of NaOH (Equation 1). The resulting solution was diluted with water to obtain a 1 mM solution of the Au(I) complex. Part of a month-old THP-Au preparation [synthesized using the bulk gold solution deformation described by Duff et al. (1993)] was passed through Sephadex equilibrated with the resulting 1 mM solution. G-50 column. The solution flowing from the column contains about 6μM THP-Au particles and 1mM [Au(THP) 4 ] Cl. The resulting solution, sufficient to cover the silicon substrate with immobilized DNA (prepared as described in Example 1), was applied to the substrate for 10 min. Thereafter, the substrate is rinsed with water and dried. The AFM images revealed mostly DNA molecules covered with THP-Au particles ...
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