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Collagen fibre extracting method and manufacture of tissue filling material therefrom

A collagen fiber and extraction method technology, applied in the field of collagen fiber extraction, can solve the problems of limited clinical application, high product viscosity, low extraction rate, etc.

Inactive Publication Date: 2003-04-02
王由
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The extract of the existing collagen extraction method is soluble collagen. Due to the strong limitation of the source of animal materials, the extraction process is complicated, a cross-linking agent needs to be added, the product has high viscosity, the extraction rate is not high, and the collagen film cannot be made, so the clinical application is very Restricted

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 The newly slaughtered cattle were washed and peeled, and 500 grams of tendons were taken, soaked in 2N hydrochloric acid for 48 hours at room temperature; The temperature was 20° C., and the cells were placed in 0.1% pepsin solution for 12 hours; the tissue to be used was taken out and washed with water to obtain an acellular collagen framework. Cut the acellular collagen framework into tissue blocks with a size of 0.05cm---0.5cm; repeat the above acid leaching and enzymolysis steps once; grind the tissue blocks to a diameter of 10μm---50μm and a length of 300μm under the monitoring of a polarizing microscope ---500μm collagen fiber filaments. A total of 2000 g of product was obtained.

[0024] Take a certain volume of collagen filament product and dry it at a constant temperature between 50°C and 90°C, weigh it, and calculate the content to be 400 mg / ml.

[0025] The following component identification experiments were performed using the collagen fibers obt...

Embodiment 2

[0030] Example 2 Newly slaughtered pigs were washed and dehaired, and 500 grams of full-thickness skin without fat on the back was taken, soaked in 10N acetic acid for 68 hours at room temperature; the tissues to be used were taken out, washed with distilled water, neutralized with 0.5N alkali, and then It was soaked in 0.2% pepsin solution at a temperature of 4°C for 24 hours; the tissue to be used was taken out and washed with water to obtain an acellular collagen framework. Cut the acellular collagen framework into tissue blocks with a size of 0.05cm---0.5cm; repeat the above acid leaching and enzymolysis steps once; 300μm---500μm collagen fibers, a total of 2000 grams of products were obtained.

Embodiment 3

[0031] Example 3 According to the method described in Example 1, wherein the acid leaching was immersed in 3N hydrochloric acid for 72 hours, and the enzymatic hydrolysis was immersed in 1.5% pepsin solution for 18 hours.

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Abstract

A process for extracting collagen fibres includes such steps as immersing animal tissue in high-penetrability acid, washing to remove acid, enzymolyzing and grinding. A process for preparing tissue filler includes such steps as diluting said collagen fibres, packing, sealing and sterilizing to obtain the filler for soft tissue, or die pressing said collagen fibres, drying and sterilizing to obtain the filler for hard dissue. Its advantages are high output rate up to more than 80% and quality, and long storage period.

Description

technical field [0001] The present invention relates to a method for extracting collagen fibers, a method for manufacturing soft tissue fillers with collagen fibers, and a method for manufacturing hard tissue fillers with collagen fibers. Background technique [0002] Currently, tissue fillers are divided into soft tissue fillers and hard tissue fillers. Animal-derived injectable collagen has been used clinically for many years as a soft tissue filler, while collagen membranes as a hard tissue filler are generally difficult to prepare from collagen extracts. [0003] Collagen fibers are harder and have higher tension. A collagen molecule is 1.5 μm in diameter and 280 μm in length, and consists of three α-configuration polypeptide chains; each chain contains 1052 amino acid residues, of which glycine accounts for one-third, proline and hydroxyproline each account for In one quarter, every second amino acid has a glycine, and glycine forms a three-amino acid combination with...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L33/12C08H1/00
Inventor 王由李永岚王维民
Owner 王由