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Collagen fibre extracting method and manufacture of tissue filling material therefrom

A collagen fiber and extraction method technology, applied in the field of collagen fiber extraction, can solve the problems of complex extraction process, limited clinical application, high viscosity of products, etc.

Inactive Publication Date: 2006-01-11
王由
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The extract of the existing collagen extraction method is soluble collagen. Due to the strong limitation of the source of animal materials, the extraction process is complicated, a cross-linking agent needs to be added, the product has high viscosity, the extraction rate is not high, and the collagen film cannot be made, so the clinical application is very Restricted

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Wash and peel the newly slaughtered cattle, take 500 grams of tendon, and soak it with 2N hydrochloric acid for 48 hours at room temperature; take out the tissue to be used, wash it with distilled water, neutralize the acid with 0.5N alkali, and then soak it in The temperature is 20° C., 0.1% pepsin solution for 12 hours; the tissue to be used is taken out and washed with water to obtain a cell-free collagen framework. Cut the acellular collagen framework into 0.05cm---0.5cm tissue blocks; repeat the above acid leaching and enzymatic hydrolysis steps once; grind the tissue blocks into diameters of 10μm---50μm and lengths of 300μm under the monitoring of a polarizing microscope ---500μm collagen fibrils. A total of 2000 g of product was obtained.

[0024] Take a certain volume of collagen filament product and dry it to a constant temperature between 50°C and 90°C. After weighing, the content is 400mg / ml.

[0025] The following component identification experim...

Embodiment 2

[0036] Example 2 Wash and remove the hair from the freshly slaughtered pig, take 500 grams of fat-free full-thickness skin on the back, and soak it with 10N acetic acid for 68 hours at room temperature; take out the tissue to be used, wash it with distilled water, neutralize it with 0.5N alkali, and then Soak it in a 0.2% pepsin solution at a temperature of 4°C for 24 hours; take out the tissue to be used and wash it with water to obtain a cell-free collagen framework. Cut the acellular collagen framework into 0.05cm---0.5cm tissue blocks; repeat the above acid leaching and enzymatic hydrolysis steps once; 300μm---500μm collagen fiber filaments, a total of 2000 grams of products were obtained.

Embodiment 3

[0037] Embodiment 3 According to the method described in Embodiment 1, wherein pickling adopts 3N hydrochloric acid to soak for 72 hours, and enzymolysis adopts pepsin solution with a concentration of 1.5% to soak for 18 hours.

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Abstract

A process for extracting collagen fibres includes such steps as immersing animal tissue in high-penetrability acid, washing to remove acid, enzymolyzing and grinding. A process for preparing tissue filler includes such steps as diluting said collagen fibres, packing, sealing and sterilizing to obtain the filler for soft tissue, or die pressing said collagen fibres, drying and sterilizing to obtain the filler for hard dissue. Its advantages are high output rate up to more than 80% and quality, and long storage period.

Description

technical field [0001] The invention relates to a method for extracting collagen fibers, a method for making soft tissue fillers with collagen fibers, and a method for making hard tissue fillers with collagen fibers. Background technique [0002] Currently, tissue fillers are divided into soft tissue fillers and hard tissue fillers. Animal-derived injectable collagen as a soft tissue filler has been clinically used for many years, but it is generally difficult to prepare from collagen extracts as a hard tissue filler. [0003] Collagen fibers are relatively stiff and have high tension. A collagen molecule has a diameter of 1.5 μm and a length of 280 μm, consisting of three polypeptide chains in α configuration; each chain contains 1052 amino acid residues, of which glycine accounts for one third, proline and hydroxyproline each account for One quarter, every second amino acid has a glycine, and glycine and proline, hydroxyproline or other amino acids form a three-amino aci...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08H1/00A61L33/12
Inventor 王由李永岚王维民
Owner 王由