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Immobilized beta-cyclodextrin and method for removing cholesterol and triglyceride

A technology of cyclodextrin and chitosan, which is applied in the field of immobilization of β-cyclodextrin, can solve problems such as obstacles and reduce application value, and achieve the effects of easy separation, low water swelling rate and hard texture

Inactive Publication Date: 2003-04-23
吴文腾
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] In 1998, Sreenivasan used chitosan powder to immobilize β-cyclodextrin, but due to the high temperature and long-term violent reaction process (60 ° C, 6 hours), it would lead to chitosan Rapid swelling (swelling) becomes a colloidal substance, which causes a very large obstacle to the adsorption of cholesterol, thus reducing its commercial application value

Method used

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  • Immobilized beta-cyclodextrin and method for removing cholesterol and triglyceride
  • Immobilized beta-cyclodextrin and method for removing cholesterol and triglyceride

Examples

Experimental program
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Effect test

Embodiment 1

[0047] Preparation of chitosan carrier

[0048] Chitosan powder (deacetylation degree 86%, molecular weight 800k Dalton) was dissolved in 1wt% acetic acid solution at a ratio of 3:100 (w / v) (g / ml), and after stirring evenly, it was injected The method (the needle number is 27G) is slowly dropped into 1N sodium hydroxide aqueous solution containing 26wt% ethanol to form white colloidal particles. Wash the colloidal particles with deionized water until neutral, and dry at room temperature for more than 36 hours to prepare a golden yellow chitosan carrier with a size of about 600-750 μm. The ethanol used has the effect of changing the specific gravity of the liquid, so that the formed colloids can quickly sink to the bottom of the solution, avoiding the situation where the colloids float on the liquid surface of the solution and stick to each other.

[0049] The needle numbered 23-27G can be used to form the golden yellow chitosan carrier with a size of about 500-1200 μm.

Embodiment 2

[0051] Preparation method of β-cyclodextrin solution

[0052] Dissolve β-cyclodextrin into N, N-dimethylformamide (N, N-dimethylformamide) solvent to form β-cyclodextrin solution, the total concentration (w / v) (g / ml) of this solution is determined by It is prepared to be twice the amount (gram weight) of the chitosan carrier reacted with it.

Embodiment 3

[0054] Method for immobilizing β-cyclodextrin on chitosan carrier

[0055] Before immobilization, the chitosan carrier prepared in Example 1 was washed once with toluene solvent, and then placed in a toluene solvent whose liquid level was sufficient to cover the chitosan carrier. Add liquid 1,6-diisocyanatohexane (1,6-diisocyanatohexane) to the toluene solvent containing the carrier, so that the ratio of its total concentration (v / v) to the chitosan carrier is 5:1 (% / w), and add 10 drops of 2-ethylhexanoic acid stannous (stannous 2-ethylhexanote) catalyst, pour out the solution after reacting at room temperature for 40 minutes, and dry the chitosan carrier with nitrogen.

[0056] The β-cyclodextrin solution prepared in Example 2 is poured on the above-mentioned carrier that has completed the grafting reaction of 1,6-diisocyanate group hexane, and 10 drops of stannous 2-ethylhexanoate catalyst are added. After reacting at room temperature for 90 minutes, pour out the solution...

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Abstract

The invented immobilization technology of beta-cyclodextrin (including chemical grafting process and physical blending process) not only can fix beta-cyclodextrin to the chitosan carrier to adsorb the cholesterine and triglyceride being in the food or blood plasma, but also can utilzie desorption technology to desorb the triglyceride and cholesterine from beta-cyclodextrin on the chitosan carrier. The desorbed triglyceride and cholesterine can be recovered, and can be used for develop by-product, for example, can be used as feed, and the chitosan carrier also can be reused by make adsorption.

Description

technical field [0001] The present invention relates to a method for immobilizing beta-cyclodextrin (beta-cyclodextrin), and a method for removing cholesterol and triglyceride in a biological substance by immobilizing beta-cyclodextrin. Background technique [0002] The relationship between dietary cholesterol (cholesterol) and total serum cholesterol has been widely discussed, and the medical community has also confirmed that excessive cholesterol and triglycerides in the blood will easily cause diseases such as arteriosclerosis. Therefore, in addition to using drugs to lower the amount of cholesterol in the body, lowering the cholesterol content in food has also become one of the ways to prevent arteriosclerosis, which is the so-called "food therapy". However, since taking medicine is a burden to the body for most people, its side effect is another kind of harm to the body, so reducing the cholesterol content in food or removing cholesterol in vitro (i...

Claims

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Application Information

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IPC IPC(8): B01D15/00B01J20/22
Inventor 吴文腾钟次文邱少华
Owner 吴文腾
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