External preparation for skin and beautifying agents
A technology for external skin preparations and whitening agents, which can be used in skin care preparations, skin diseases, cosmetic preparations, etc., and can solve problems such as instability, insufficient whitening effect, and easy oxidation.
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[0209] The present invention will be described with reference to the following examples, but the invention is not limited at all by these examples.
[0210] For the pentacyclic triterpene compounds used in the examples, erythrodiol (available from Funakoshi Company), ursyl alcohol (available from Funakoshi Company), betulinic acid (available from Funakoshi Company), and betulin (available from Funakoshi Company) ) are purchased reagents. Herein, those of HPLC grade were used without any post-treatment, while the remaining reagents were dissolved in ethanol and added to its boiling point until the resulting solution reached saturation, followed by recrystallization by cooling, filtering and drying the recrystallized products, and they were used thereafter. As will be described as an example in the Examples, the maslinic acid used here is extracted and purified from olive plants. The purity of the product was confirmed to be 95%. [Preparation Example 1]
preparation example 1
[0211] Dried fruit (including seeds) (500 g) of natural olive (Olea europaea L.) was crushed and 3 L of hexane was added to the crushed product, and the dried fruit was extracted for 3 hours. Repeat above-mentioned operation 4 times to obtain defatted fruit, remove the seed in the fruit, the fruit thus processed is pulverized and the fruit is extracted again with 5 volumes of hexane for 3 hours to obtain 229g of defatted residue, in which the oil components are all removed. To this defatted residue was added 10 volumes of aqueous ethanol having an ethanol content of 60% by weight, followed by extraction at room temperature for 3 hours with vigorous stirring. The entire extraction system was filtered, and the resulting filtrate was concentrated to dryness to obtain 112.7 g of an extract.
[0212] To 100 g of this extract was added 2 L of water, followed by vigorous stirring at room temperature for 1 hour. The entire mixture was centrifuged, the resulting supernatant was remove...
preparation Embodiment 2
[0216] Olive plants (Olea europea L.) grown in Italy were subjected to oil extraction to obtain 1 kg of oil extraction residue, to which was added 10 volumes of aqueous ethanol with an ethanol content of 65% by weight, and the extraction was carried out at room temperature while Stir vigorously. The entire extraction system was filtered, and the filtrate was concentrated to dryness to obtain 20.2 g of the extracted product.
[0217] 1 L of n-butanol and 1 L of water were added to the extract, and stirred for 10 minutes, whereby the mixture was partitioned into the n-butanol phase and the water phase. The n-butanol of the n-butanol phase was removed, followed by vacuum drying to obtain 13.3 g of a concentrate.
[0218] Further, the concentrate was fractionated through a silica gel column with a column loaded with about 40 volumes (500 g) of silica gel. First use 3:1 hexane-ethyl acetate mixed solvent as eluent to elute various unwanted components with 10 times the volume (500...
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